纺织学报 ›› 2020, Vol. 41 ›› Issue (05): 15-19.doi: 10.13475/j.fzxb.20190502306

• 纤维材料 • 上一篇    下一篇

聚羟基脂肪酸酯/海藻酸钠纳米纤维的制备及其性能

孙范忱1, 郭静1,2, 于跃1,2, 张森1,2   

  1. 1.大连工业大学 纺织与材料工程学院, 辽宁 大连 116034
    2.辽宁省功能纤维及复合材料工程技术中心, 辽宁 大连 116034
  • 收稿日期:2019-05-13 修回日期:2020-02-08 出版日期:2020-05-15 发布日期:2020-06-02
  • 作者简介:孙范忱(1993—),男,硕士。主要研究方向为高分子材料改性与加工。
  • 基金资助:
    国家自然科学基金项目(51773024,51373027);辽宁省科技创新团队项目(LT2017017)

Preparation and properties of polyhydroxy fatty acid ester/sodium alginate composite electrospun nanofibers

SUN Fanchen1, GUO Jing1,2, YU Yue1,2, ZHANG Sen1,2   

  1. 1. School of Textile and Material Engineering, Dalian Polytechnic University, Dalian, Liaoning 116034, China
    2. Liaoning Provincial Engineering Research Center of Functional Fiber and Its Composite Materials, Dalian, Liaoning 116034, China
  • Received:2019-05-13 Revised:2020-02-08 Online:2020-05-15 Published:2020-06-02

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摘要：

针对聚羟基脂肪酸酯(P(3HB-co-4HB))和海藻酸钠(SA)常规情况下难共溶的问题,以P(3HB-co-4HB)为原料,SA为改性剂,三氯甲烷/水为溶剂,烷基糖苷(APG)为乳化剂,通过共混利用静电纺丝法制备了P(3HB-co-4HB)/SA纳米纤维膜。借助红外光谱仪、差示扫描量热仪、扫描电子显微镜表征P(3HB-co-4HB)/SA静电纺纳米纤维的分子间作用力、热性能和形貌;利用细胞毒性和细胞共培养测试表征了P(3HB-co-4HB)/SA纳米纤维的生物相容性。结果表明:P(3HB-co-4HB)/SA复合材料的玻璃化转变温度发生改变,当添加SA质量分数为6%时,静电纺纳米纤维具有均一的形貌,纳米纤维的平均直径为500 nm,孔隙率为74%,细胞毒性等级为0级,P(3HB-co-4HB)和SA具有良好的生物相容性。

关键词: 聚羟基脂肪酸酯, 海藻酸钠, 纳米纤维, 生物相容性, 静电纺丝

Abstract:

Aiming at the problem that polyhydroxy fatty acid ester (P(3HB-co-4HB)) and sodium alginate (SA) are difficult to co-solve under normal circumstances, a P(3HB-co-4HB)/SA nanofiber membrane were prepared using P(3HB-co-4HB) as raw material, SA as modifier, chloroform/water as solvent, alkyl glycoside as emulsifier. The intermolecular forces, thermal properties and morphology of P(3HB-co-4HB)/SA electrospinning nanofibers were characterized by infrared spectrometer, differential scanning calorimeter, and scanning electron microscope. The biocompatibility of P(3HB-co-4HB)/SA nanofibers was characterized by cytotoxicity test and cell co-culture. The results show that the formation of the P(3HB-co-4HB)/SA composite material caused changes in glass transition temperature. When the mass fraction of SA is 6%, the electrospinning nanofibers have a uniform morphology, and the average diameter of the nanofibers is 500 nm. It demonstrates a porosity of 74% and the grade 0 cytotoxicity, and the P(3HB-co-4HB))and SA has good biocompatibility.

Key words: polyhydroxy fatty acid ester, sodium alginate, nanofiber, biocompatibility, electrospinning

中图分类号: 

  • TB332

图1

实验机制和实验过程"

图2

SA、P(3HB-co-4HB) 和P(3HB-co-4HB)/SA的红外光谱图"

图3

氢键结构示意图"

图4

P(3HB-co-4HB)和P(3HB-co-4HB)/SA的DSC曲线"

图5

不同SA质量分数纤维膜的扫描电镜照片(×5 000)"

表1

不同SA质量分数纤维膜的直径和孔隙率"

SA质量分数/% 平均直径/μm 孔隙率/%
2
4
6
8
10
12		 0.47±0.02
0.49±0.02
0.50±0.01
0.52±0.03
0.37±0.11
0.42±0.20		 77.0±0.14
75.8±0.09
74.0±0.07
70.5±0.12
89.0±0.23
82.0±0.21

表2

不同SA质量分数纤维膜浸提液的吸光度值"

时间/h 空白样 不同SA质量分数时的吸光度
0% 2% 4% 6% 8% 10% 12%
12
24
36
48
72
96		 0.302±0.102
0.412±0.109
0.789±0.193
1.293±0.170
1.621±0.142
2.060±0.175		 0.300±0.102
0.403±0.111
0.769±0.122
1.300±0.155
1.600±0.124
2.011±0.155		 0.323±0.139
0.436±0.147
0.796±0.183
1.329±0.170
1.659±0.187
2.075±0.037		 0.339±0.050
0.439±0.138
1.028±0.114
1.408±0.164
1.768±0.061
2.374±0.166		 0.312±0.041
0.439±0.124
0.875±0.138
1.399±0.095
1.768±0.190
2.301±0.108		 0.321±0.036
0.476±0.097
0.888±0.199
1.395±0.181
1.649±0.125
2.210±0.187		 0.327±0.074
0.429±0.079
0.800±0.145
1.410±0.181
1.764±0.100
2.367±0.160		 0.347±0.056
0.450±0.151
0.875±0.136
1.391±0.168
1.835±0.112
2.369±0.109

图6

不同SA质量分数纤维膜的体外细胞培养扫描电镜照片(×500)"

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