纺织学报 ›› 2021, Vol. 42 ›› Issue (04): 114-120.doi: 10.13475/j.fzxb.20200703707

• 染整与化学品 • 上一篇    下一篇

乌拉草提取液中木犀草素含量的测定及其抗菌性能

王春红1,2(), 杨璐1, 胡敏1, 王晓云1, 王利剑1   

  1. 1.天津工业大学 纺织科学与工程学院, 天津 300387
    2.天津工业大学 先进纺织复合材料重点实验室, 天津 300387
  • 收稿日期:2020-07-15 修回日期:2020-12-31 出版日期:2021-04-15 发布日期:2021-04-20
  • 作者简介:王春红(1980—),女,教授,博士。主要研究方向为天然资源利用与绿色化应用。E-mail: wangchunhong@tiangong.edu.cn
  • 基金资助:
    国家自然科学基金项目(11802205)

Determination of luteolin content in carex meyeriana extract and its antibacterial properties

WANG Chunhong1,2(), YANG Lu1, HU Min1, WANG Xiaoyun1, WANG Lijian1   

  1. 1. School of Textile Science and Engineering, Tiangong University, Tianjin 300387, China
    2. Key Laboratory of Advanced Textile Composite Materials, Tiangong University, Tianjin 300387, China
  • Received:2020-07-15 Revised:2020-12-31 Online:2021-04-15 Published:2021-04-20

摘要:

为充分开发乌拉草的应用价值,探究其抗菌性能,采用水醇提取法制备了乌拉草提取液,研究了不同醇水比提取液的基本性能。利用紫外分光光度法和高效液相色谱法测试乌拉草提取液中抗菌物质木犀草素的含量,并对2种测试结果进行比较,采用体外抗菌法测试其抑菌率。结果表明:随着提取溶剂中水的比例的增加,提取液的电导率逐渐增大,pH值逐渐减小且均呈现酸性;紫外分光光度法及高效液相色谱法得出的木犀草素含量变化趋势一致,随着提取溶剂中水的比例的增大,木犀草素的含量均逐渐减少;不同醇水比的4种样品对大肠杆菌及金黄色葡萄球菌的抑菌率均大于70%,表明乌拉草提取液具有良好的抗菌性。

关键词: 乌拉草提取液, 紫外分光光度法, 高效液相色谱法, 木犀草素, 体外抗菌活性, 抗菌性能

Abstract:

In order to develop the application of carex meyeriana, and explore its antibacterial performance, the carex meyeriana extract was extracted by alcohol water mixture, and the basic performance of the extract with different alcohol water ratios were tested. The content of luteolin, an antibacterial substance, was tested by ultraviolet (UV) spectrophotometer and high performance liquid chromatography (HPLC) method, and the results of the two tests are compared. Its antibacterial rate was tested by in vitro antibacterial method. The results show that with the increase of the proportion of water in the extraction solvent, the conductivity of the extraction solution gradually increases and the pH value gradually decreases exhibiting acidity. The change trends of luteolin content detected by using UV spectrophotometer and HPLC method are the same. The content of luteolin decreases with the increase of the proportion of water in the extraction solvent, and the antibacterial rates of Escherichia coli and Staphylococcus aureus in the four samples with different alcohol water ratios were all higher than 70%, which indicates that the extract has good antibacterial properties.

Key words: carex meyeriana extract, ultraviolet spectrophotometry method, high performance liquid chromatography method, luteolin, in vitro antibacterial activity, antibacterial property

中图分类号: 

  • TS101.4

表1

培养基配制方法"

培养基名称 牛肉膏
质量/g
蛋白胨
质量/g
溶剂
名称 体积/mL
A 0.6 1.0 提取液a 200
B 0.6 1.0 提取液b 200
C 0.6 1.0 提取液c 200
D 0.6 1.0 提取液d 200
E 0.9 1.5 蒸馏水 300

表2

不同提取液的pH值和电导率测试结果"

提取液编号 pH值 电导率/(μS·cm-1)
a 6.39 192
b 6.26 322
c 5.94 471
d 5.69 546

图1

提取液的表观颜色图"

图2

木犀草素标准溶液紫外分光测试曲线"

表3

采用紫外分光光度法测得的不同溶剂提取液中木犀草素的质量浓度"

提取液编号 吸光度 木犀草素质量浓度/(μg·mL-1)
a 1.375 29.22
b 0.123 1.36
c 0.121 1.31
d 0.110 1.07

图3

木犀草素标准溶液高效液相色谱图"

图4

木犀草素标准溶液高效液相色谱标准曲线"

图5

不同提取液样品高效液相色谱图"

表4

高效液相色谱法得到的不同提取液中木犀草素的质量浓度"

提取液编号 峰面积/au·min 木犀草素的质量浓度/(μg·mL-1)
a 3 803 864 37.97
b 182 512 1.48
c 172 481 1.38
d 145 449 1.11

图6

不同醇水比乌拉草提取液对大肠杆菌的抗菌效果图"

表5

乌拉草提取液对大肠杆菌的抑菌率"

平板序号 细菌计数平均值/(109 CFU·mL-1) 抑菌率/%
实验组A 4.24 90.6
实验组B 8.73 80.5
实验组C 9.40 79.2
实验组D 11.10 75.5
对照组E 45.30

图7

不同醇水比乌拉草提取液对金黄色葡萄球菌抗菌效果图"

表6

乌拉草提取液对金黄色葡萄球菌的抑菌率"

平板序号 细菌计数平均值/(109 CFU·mL-1) 抑菌率/%
实验组A 8.93 88.6
实验组B 14.70 81.2
实验组C 19.60 75.0
实验组D 19.90 74.6
对照组E 78.30
[1] 周淑荣, 董昕瑜, 包秀芳, 等. 中国东北地区乌拉草资源及开发利用[J]. 特种经济动植物, 2013,16(6):37-40.
ZHOU Shurong, DONG Xinyu, BAO Xiufang, et al. There sources and exploitation of rabdosia uralensis in northeast China[J]. Special Economic Animals and Plants, 2013,16(6):37-40.
[2] 胡敏, 王春红, 荆妙蕾, 等. 乌拉草纤维的研究进展[J]. 棉纺织技术, 2020,48(3):80-84.
HU Min, WANG Chunhong, JING Miaolei, et al. Research progress of carex meyeriana kunth fiber[J]. Cotton Textile Technology, 2020,48(3):80-84.
[3] WANG H, NIE L, XU Y, et al. Traffic-emitted metal status and uptake by carex meyeriana kunth and thelypteris palustris var pubescens fernald growing in roadside turfy swamp in the Changbai mountain area, China[J]. Environmental Science and Pollution Research, 2018,25(19):18498-18509.
pmid: 29696547
[4] 董立琴, 骆倩, 蒋洁, 等. 几种新型天然纤维素纤维研究进展[J]. 纺织科技进展, 2017,39(2):6-11.
DONG Liqin, LUO Qian, JIANG Jie, et al. Research progress of several new natural cellulose fibers[J]. Progress in Textile Science & Technology, 2017,39(2):6-11.
[5] 王利军, 王春红, 龙碧璇, 等. 乌拉草/棉/维纶复合纱线的开发与性能[J]. 纺织学报, 2017,38(12):49-53.
WANG Lijun, WANG Chunhong, LONG Bixuan, et al. Development and performance of carex meyeriana/cotton/vinylon composite yarn[J]. Journal of Textile Research, 2017,38(12):49-53.
[6] 王利军, 王春红, 王妮, 等. 乌拉草纤维的提取及其结构和性能研究[J]. 上海纺织科技, 2017,45(10):22-25,64.
WANG Lijun, WANG Chunhong, WANG Ni, et al. Extraction of meyer sedge fiber and its structure and properties[J]. Shanghai Textile Science & Technology, 2017,45(10):22-25,64.
[7] YANG X, LIU H, HAN F Y, et al. Fabrication of cellulose nanocrystal from carex meyeriana kunth and its application in the adsorption of methylene blue[J]. Carbohydrate Polymers, 2017,175(1):464-472.
doi: 10.1016/j.carbpol.2017.08.007
[8] 董伟楠. 多级孔结构碳材料在超级电容器中的电化学性能研究[D]. 锦州:渤海大学, 2020: 19-32.
DONG Weinan. Research of the electrochemical performance of hierarchical porous carbon materials in supercapacitors[J]. Jinzhou:Bohai University, 2020: 19-32.
[9] 余克娇. 乌拉草化学成分和药理作用的初步研究[D]. 沈阳:沈阳药科大学, 2005: 10-14.
YU Kejiao. Preliminary inquiry of the chemical constituents and pharmacological effects in the curaua[D]. Shenyang:Shenyang Pharmaceutical University, 2005: 10-14.
[10] 蔡蕙荞, 杨子珍, 解玉, 等. 黄酮类化合物木犀草素对哮喘影响的研究近况[J]. 世界最新医学信息文摘, 2019,19(50):89-90.
CAI Huiqiao, YANG Zizhen, XIE Yu, et al. Recent research on the effect of luteolin on asthma[J]. World Latest Medicine Information, 2019,19(50):89-90.
[11] 王伟, 何平, 江小明. 木犀草素及其黄酮苷的抗炎、抗氧化作用[J]. 食品科学, 2020,41(17):208-215.
WANG Wei, HE Ping, JIANG Xiaoming. Anti-inflammatory and antioxidant effects of luteolin and its flavone glycosides[J]. Food Science, 2020,41(17):208-215.
[12] 于倩, 巫冠中. 木犀草素抗炎机制的研究进展[J]. 药学研究, 2019,38(2):108-111.
YU Qian, WU Guanzhong. Research progress of anti-inflammatory properties of luteolin[J]. Journal of Pharmaceutical Research, 2019,38(2):108-111.
[13] 夏云岭, 张振凌, 张洪坤, 等. HPLC法同时测定半枝莲饮片中4种黄酮类成分的含量及主成分分析[J]. 中国药房, 2019,30(20):2839-2844.
XIA Yunling, ZHANG Zhenling, ZHANG Hongkun, et al. Content determination and principle components analysis of 4 kinds of flavones in scutellaria barbata decoction pieces by HPLC[J]. China Pharmacy, 2019,30(20):2839-2844.
[14] 马梦雪, 吴士筠, 李刚, 等. HPLC法同时测定不同品种红花中4种黄酮类[J]. 中成药, 2019,41(11):2694-2700.
MA Mengxue, WU Shiyun, LI Gang, et al. Simultaneous determination of four flavonoids in carthamus tinotorius of different varieties by HPLC[J]. Chinese Traditional Patent Medicine, 2019,41(11):2694-2700.
[15] 林卓慧. 木犀草素抗菌活性研究[J]. 检验医学与临床, 2009,6(12):1022.
LIN Zhuohui. Study on the antibacterial activity of luteolin[J]. Laboratory Medicine and Clinic, 2009,6(12):1022.
[16] 王春红, 白肃跃, 马海军, 等. 乌拉草纤维的超声波辅助碱氧一浴法提取工艺优化[J]. 农业工程学报, 2013,29(9):267-274.
WANG Chunhong, BAI Suyue, MA Haijun, et al. Optimization on ultrasound-alkali-H2O2 one-bath extraction process of curaua fiber[J]. Transactions of the Chinese Society of Agricultural Engineering, 2013,29(9):267-274.
[17] 陈莉, 李潇. 姜黄染色织物的pH值敏感变色性能[J]. 纺织学报, 2017,38(4):80-84.
CHEN Li, LI Xiao. Discoloration property for pH-sensitivity of fabric dyed by turmeric[J]. Journal of Textile Research, 2017,38(4):80-84.
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