纺织学报 ›› 2021, Vol. 42 ›› Issue (02): 41-46.doi: 10.13475/j.fzxb.20201008606

• 纤维材料 • 上一篇    下一篇

丝素蛋白/磷酸八钙复合材料生物界面的蛋白质吸附和细胞响应

杨亚1,2, 闫凤祎2, 王卉2, 张克勤2()   

  1. 1.常熟理工学院 纺织服装与设计学院, 江苏 常熟 215500
    2.苏州大学 纺织与服装工程学院, 江苏 苏州 215000
  • 收稿日期:2020-10-30 修回日期:2020-11-13 出版日期:2021-02-15 发布日期:2021-02-23
  • 通讯作者: 张克勤
  • 作者简介:杨亚(1990—),女,讲师,博士。主要研究方向为丝素蛋白生物医用材料、纳米材料和3D打印的研究。
  • 基金资助:
    江苏省基础研究计划面上研究项目(BK20161253)

Protein adsorption and cell response on bio-interfaces of silk fibroin/octacalcium phosphate composites

YANG Ya1,2, YAN Fengyi2, WANG Hui2, ZHANG Keqin2()   

  1. 1. School of Textile Garment and Design, Changshu Institute of Technology, Changshu, Jiangsu 215500, China
    2. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215000, China
  • Received:2020-10-30 Revised:2020-11-13 Online:2021-02-15 Published:2021-02-23
  • Contact: ZHANG Keqin

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摘要：

为进一步了解仿生材料结构与功能的关系,为体内骨整合设计一个有利的种植微环境,通过电化学沉积技术调控电解液中丝素蛋白(SF)的质量浓度,制备了具有纳微米多级结构的丝素蛋白/磷酸八钙(SF/OCP)复合涂层。研究了丝素蛋白质量浓度对SF/OCP复合涂层的表面形貌、力学性能、蛋白质吸附以及细胞增殖行为的影响。结果表明:随电解液中丝素蛋白质量浓度的增加,涂层表面孔洞直径由(19.96 ± 6.96) μm减小至(1.56 ± 0.22) μm, OCP晶体宽度减小至纳米级别((26.84 ± 8.2) nm);与纯OCP涂层相比,SF/OCP复合涂层(SF质量浓度为1.0 mg/mL)的弹性模量和硬度分别增加了约1.5和4.3倍;SF/OCP复合涂层选择性地增强了纤连蛋白(Fn)的吸附,经7 d的细胞培养后,SF/OCP复合涂层(SF质量浓度为1.0 mg/mL)表面的细胞活力是纯OCP涂层上的1.28倍。

关键词: 丝素蛋白, 磷酸八钙, 蛋白质吸附, 细胞培养, 仿生材料

Abstract:

To further understand the structure-function relationships for biomimetic materials for the purpose of designing a favorable implant microenvironment for osseointegration in vivo, the nano/micro hierarchical structure of silk fibroin (SF)/octacalcium phosphate (OCP) composite coating was constructed by controlling simply the SF concentration of the electrolyte during the electrochemical deposition process. The influence of SF concentration on surface morphology, mechanical property, protein adsorption and cell proliferation were studied. The results show that the pore diameter of the coating surface decreases from (19.96±6.96) μm to (1.56±0.22) μm and the crystal width decreases to the nano-scale with SF concentration increasing in electrolyte. Comparing the pure OCP coating, the elastic modulus and hardness of the SF/OCP composite coating (1.0 mg/mL SF) increase approximately 1.5 times and 4.3 times, respectively. The SF/OCP composite coating selectively enhances fibronectin (Fn) adsorption, and the cell viability on the SF/OCP composite coating (1.0 mg/mL SF) is 1.28 times that on the pure OCP coating after culturing for 7 days.

Key words: silk fibroin, octacalcium phosphate, protein adsorption, cell culture, biomimetic material

中图分类号: 

  • TS101.4

图1

纯OCP涂层和SF/OCP复合涂层电镜照片"

图2

放大的纯OCP涂层和SF/OCP复合涂层电镜照片"

图3

孔洞直径和OCP晶带宽度随丝素蛋白质量浓度的变化"

图4

SF/OCP复合涂层电化学沉积机制"

表1

制备涂层的弹性模量和硬度"

样品名称 弹性模量 硬度
OCP涂层 8.56 ± 0.62 0.011 ± 0.001
SF/OCP涂层 12.84 ± 1.34 0.047 ± 0.011

图5

BSA在纯Ti基底、纯OCP涂层和SF/OCP 复合涂层上的吸附量 注:*表示与纯Ti基底相比,p<0.05;#表示与纯OCP涂层相比,p<0.05。"

图6

Fn在纯Ti基底、纯OCP涂层和SF/OCP复合涂层上的吸附量 注:*表示与纯Ti基底相比, p<0.05; #表示与纯OCP涂层相比, p<0.05。"

图7

HUMSCs在纯OCP涂层和SF/OCP复合涂层上培养1、3和7 d时的细胞活力 注:*表示涂层表面细胞活力的显著性差异,p<0.05。"

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