新型可降解编织结构神经再生导管的制备及其性能

doi:10.13475/j.fzxb.20210902007

纺织学报 ›› 2022, Vol. 43 ›› Issue (02): 125-131.doi: 10.13475/j.fzxb.20210902007

新型可降解编织结构神经再生导管的制备及其性能

姚若彤¹^,², 赵婧媛¹, 闫一欣¹, 段立蓉¹^,², 王恬³, 严佳¹^,², 张淑军¹, 李刚¹^,²()

1.苏州大学纺织与服装工程学院, 江苏苏州 215123
2.苏州大学现代丝绸国家工程实验室,江苏苏州 215123
3.北卡罗莱纳州立大学纺织学院, 罗利 27695

收稿日期:2021-09-07 修回日期:2021-11-21 出版日期:2022-02-15 发布日期:2022-03-15
通讯作者: 李刚
作者简介:姚若彤(1999—),女,硕士生。研究方向为生物医用纺织材料。
基金资助:
国家重点研发计划项目(2021YFE0111100);中国纺织工业联合会应用基础研究项目(J202002);江苏省第十五批“六大人才高峰”高层次人才项目(GDZB-035);南通市科技计划应用基础研究项目(JC2020082);国家先进功能纤维创新中心科研攻关项目(2021-fx010104)

Fabrication of novel biodegradable braided nerve grafts for nerve regeneration

YAO Ruotong¹^,², ZHAO Jingyuan¹, YAN Yixin¹, DUAN Lirong¹^,², WANG Tian³, YAN Jia¹^,², ZHANG Shujun¹, LI Gang¹^,²()

1. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China
2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China
3. Wilson College of Textiles, North Carolina State University, Raleigh 27695, USA

Received:2021-09-07 Revised:2021-11-21 Published:2022-02-15 Online:2022-03-15
Contact: LI Gang

摘要/Abstract

摘要：

为研制具有良好力学性能和生物相容性的人工神经导管,采用编织工艺、静电纺丝技术和冷冻干燥技术制备一种含有壳聚糖涂层-编织层-纤维海绵层的3层复合结构人工神经导管,研究轴纱、内外层结构和镁离子质量浓度对其性能的影响,并对导管的表面形貌、力学性能、离子缓释性能和生物相容性进行表征。结果表明:轴纱和编织纱共同参与编织时,神经导管形变50%的径向压缩性能为1.3 N,轴纱断裂时的轴向拉伸应力为30 N,具有良好的力学性能;导管内海绵层呈相互连通的多孔结构,孔径分布均匀(0.04~0.08 mm);导管内镁离子可缓慢释放28 d;当镁离子溶液质量浓度为0.02 g/mL时,导管对细胞增殖的促进作用最显著。这为神经导管的材料选择和结构优化提供新思路。

关键词: 人工神经导管, 周围神经修复, 可降解, 丝素蛋白, 镁离子

Abstract:

In order to develop artificial nerve guidance conduits (NGCs) with good mechanical properties and biocompatibility, this paper describes a three-layer composite artificial nerve graft made up with a chitosan coating layer, a braided layer and a fibrous sponge layer using traditional braiding, electrospinning and freeze-drying techniques. The morphology, mechanical properties, biocompatibility and sustained-release properties were investigated, studying the effects of axial yarn, the outer and inner layers and magnesium ion concentration on its performance. The results showed that the prepared NGCs with braided and axial yarns possess good mechanical properties. The radial compressive property of the NGC with 50% deformation is 1.3 N and the axial tensile stress to yarn fracture is 30 N. The sponge layer features an inter-connected porous structure with uniform pore size distribution (0.04-0.08 mm). The magnesium ion in the NGC can be sustainably released for 28 d. When the concentration of magnesium ion solution is 0.02 g/mL, the NGC has the greatest effect on promoting cell proliferation. This paper provides new ideas for selecting materials for NGCs and optimizing their structural properties.

Key words: artificial nerve graft, peripheral nerve repair, biodegradability, silk fibroin, magnesium ion

中图分类号:

TS102.3

姚若彤, 赵婧媛, 闫一欣, 段立蓉, 王恬, 严佳, 张淑军, 李刚. 新型可降解编织结构神经再生导管的制备及其性能[J]. 纺织学报, 2022, 43(02): 125-131.

YAO Ruotong, ZHAO Jingyuan, YAN Yixin, DUAN Lirong, WANG Tian, YAN Jia, ZHANG Shujun, LI Gang. Fabrication of novel biodegradable braided nerve grafts for nerve regeneration[J]. Journal of Textile Research, 2022, 43(02): 125-131.

图/表 10

表1

图1

表2

含不同质量浓度镁离子的静电纺丝薄膜的元素含量"

样品中M $g 2 +$ 质量浓度/(g·mL^-1)	元素	质量分数/%	均方差	原子含量/%
0	C	67.152	0.680	73.143
	O	32.838	0.679	26.852
	Mg	0.010	0.062	0.006
0.01	C	69.003	0.607	74.850
	O	30.666	0.608	24.972
	Mg	0.310	0.067	0.177
0.015	C	69.197	0.607	75.013
	O	30.509	0.608	24.829
	Mg	0.294	0.068	0.158
0.02	C	67.662	0.611	73.790
	O	31.390	0.613	25.699
	Mg	0.948	0.079	0.511

表2

表3

图2

图3

图4

图5

图6

图7

参考文献 26

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结构名称		浓度/cm^-1	结构名称		浓度/cm^-1
	酰胺 Ⅰ	1 650~1 660		酰胺 Ⅰ	1 625~1 640
Silk Ⅰ	酰胺 Ⅱ	1 535~1 545	Silk Ⅱ	酰胺 Ⅱ	1 515~1 525
	酰胺 Ⅲ	1 253		酰胺 Ⅲ	1 235

新型可降解编织结构神经再生导管的制备及其性能

Fabrication of novel biodegradable braided nerve grafts for nerve regeneration

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