聚氮异丙基丙烯酰胺抗菌水凝胶复合棉织物的制备及其性能

doi:10.13475/j.fzxb.20230102501

纺织学报 ›› 2024, Vol. 45 ›› Issue (05): 129-137.doi: 10.13475/j.fzxb.20230102501

聚氮异丙基丙烯酰胺抗菌水凝胶复合棉织物的制备及其性能

薛宝霞¹^,²^,³, 杨色¹^,², 张春艳¹^,², 刘晶¹^,², 刘勇³, 程伟³, 张利³, 牛梅¹^,²()

1.太原理工大学轻纺工程学院, 山西太原 030024
2.山西浙大新材料与化工研究院, 山西太原 030032
3.山西白求恩医院(山西医学科学院同济山西医院), 山西太原 030032

收稿日期:2023-01-13 修回日期:2024-01-15 出版日期:2024-05-15 发布日期:2024-05-31
通讯作者: 牛梅(1979—),女,教授,博士。研究方向为功能化与差别化新型纤维的设计。E-mail:niumei@tyut.edu.cn。
作者简介:薛宝霞(1990—),女,副教授,博士。主要研究方向为纳米智能抗菌功能材料。
基金资助:
山西省自然科学基金项目(20210302124200);山西省自然科学基金项目(202103021224355);山西省自然科学基金项目(202203021211065);山西浙大新材料与化工研究院资助项目(2021SX-TD013);山西浙大新材料与化工研究院资助项目(2021SX-FR010);山西纳米药物可控缓释技术创新中心项目(20210410911026);山西省高等学校科技创新项目(2021L027)

Preparation and properties of cotton fabric with poly(N-isopropylacrylamide) antibacterial hydrogel

XUE Baoxia¹^,²^,³, YANG Se¹^,², ZHANG Chunyan¹^,², LIU Jing¹^,², LIU Yong³, CHENG Wei³, ZHANG Li³, NIU Mei¹^,²()

1. College of Textile Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 030024, China
2. Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering, Taiyuan, Shanxi 030032, China
3. Shanxi Bethune Hospital (Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital), Taiyuan, Shanxi 030032, China

Received:2023-01-13 Revised:2024-01-15 Published:2024-05-15 Online:2024-05-31

摘要/Abstract

摘要：

为扩大含银抗菌水凝胶的应用和实现缓释抗菌,采用水凝胶复合织物的构建策略,将化学交联与紫外光引发相结合,通过聚氮异丙基丙烯酰胺(PNIPAM)/载银氧化石墨烯(GO-Ag)抗菌水凝胶复合棉织物,形成新型水凝胶复合织物一体化敷料,并探讨不同层数棉织物复合水凝胶对敷料结构与抗菌性能、拉伸性能以及生物安全性等的影响。结果表明:PNIPAM/GO-Ag抗菌水凝胶与3层棉织物构筑的一体化敷料的综合性能优异;在湿态下,一体化敷料比单一棉织物的断裂强力提升73.7%,达到370 N;敷料的体外细胞毒性呈0级,溶血率小于5%,显示出良好的生物安全性;其对大肠杆菌和金黄色葡萄球菌的抗菌率均达到98%以上,在24 h内具有缓慢释放银离子的效果;抗菌作用主要通过持续释放银离子、诱导细菌产生活性氧造成其氧化损伤等多种作用,从而破坏细菌细胞结构。

关键词: 聚氮异丙基丙烯酰胺, 水凝胶, 缓释, 抗菌, 纳米银, 棉织物, 敷料

Abstract:

Objective In order to reduce the incidence of chronic wound infections, various antibacterial dressings play an important role in wound treatment, among which silver-based antibacterial hydrogel is advantageous. However, sudden release of silver antibacterial agent and poor mechanical properties of hydrogel are the two factors limit lug the application of silver-based antibacterial hydrogel in wound repair. In order to expand the application of silver-based antibacterial hydrogel and achieve sustained release antibacterial, the construction strategy of hydrogel composite fabric was adopted to prepare a new type of hydrogel composite fabric integrated dressing.

Method Combined with the chemical crosslinking and ultra violet light initiation method, the polyazoiso propyl acrylamide (PNIPAM) /silver loaded with graphene oxide (GO-Ag) hydrogel was combined with cotton fabric to form a new kind of dressing with the integration of hydrogel and fabric. The number of cotton fabric layer used for constructing dressing was explored. The structure, antibacterial properties, tensile strength, and biological safety of dressings were discussed by scanning electron microscopy, infrared spectroscopy, fluorescence inverted microscopy, etc.

Results Based on research of PNIPAM/GO-Ag composite antibacterial hydrogel, the PNIPAM/GO-Ag composite cotton fabric with different layers was constructed. According to the analysis results of microscopic morphology and molecular structure, the PNIPAM/GO-Ag hydrogel was evenly interpenetrated into cotton fabric because of the strong interface integration. Analyzing the antibacterial, mechanical, and biological properties of PNIPAM/GO-Ag hydrogel composite cotton fabric with different layers, it was found that the integrated dressing, composed of PNIPAM/GO-Ag hydrogel and three-layer cotton fabric, demonstrated excellent comprehensive performance. In contrast with the single cotton fabric, the tensile breaking strength of the integrated dressing were improved by 73.7% and reached 370 N in the wet state. This proved that the integrated structure of hydrogel composite cotton fabric could help strengthen the mechanical property of hydrogel, which is beneficial for the promotion and application of wound dressing. The in vitro cytotoxicity of dressing was 0 level, and the hemolysis rate was less than 5%, demonstrating the PNIPAM/GO-Ag hydrogel composite cotton fabric illustrated good biological safety, providing possibilities for its application in the field of wound repair. The inhibition rate of antibacterial dressing on the Escherichia coli and Staphylococcus aureus reached more than 98%. Compared with the slow-release antibacterial effect of PNIPAM/GO-Ag hydrogel, the PNIPAM/GO-Ag hydrogel composite cotton fabric developed slow-release effect of silver within 24 h, proving that the hydrogel composite fabric has a more durableability to continuously release silver release silver ions, and the slow-release antibacterial effect of dressing was enhanced. In addition, from the analysis of bacterial micro-structure and intracellular reactive oxygen species (ROS) content, it was concluded that the antibacterial effect of hydrogel composite cotton fabric was mainly achieved by continuously releasing silver ions, inducing bacteria to produce ROS and causing oxidative damage to bacteria, thereby destroying bacteria.

Conclusion The combination strategy of hydrogel composite fabric is one of the methods to construct a new integrated antibacterial dressing, which has the characteristics of both hydrogel and fabric. It was found that the interface between the hydrogel and cotton fabric directly affects the structural integrity of the overall dressing. The number of cotton fabric layer determines the interface binding force between the hydrogel and each layer of the fabric, and ultimately affects its tensile properties, antibacterial properties, and biological safety. Compared with the single cotton fabric, in the wet state, the tensile breaking strength of PNIPAM/GO-Ag composite cotton fabric integrated dressing has strengthened. And the slow-release antibacterial effect of silver ions in PNIPAM/GO-Ag composite cotton fabric is more pronounced than that of PNIPAM/GO-Ag hydrogel.

Key words: polyazoisopropylacrylamide, hydrogel, slow release, antibacterial, nano sliver, cotton fabric, dressing

中图分类号:

TS115

薛宝霞, 杨色, 张春艳, 刘晶, 刘勇, 程伟, 张利, 牛梅. 聚氮异丙基丙烯酰胺抗菌水凝胶复合棉织物的制备及其性能[J]. 纺织学报, 2024, 45(05): 129-137.

XUE Baoxia, YANG Se, ZHANG Chunyan, LIU Jing, LIU Yong, CHENG Wei, ZHANG Li, NIU Mei. Preparation and properties of cotton fabric with poly(N-isopropylacrylamide) antibacterial hydrogel[J]. Journal of Textile Research, 2024, 45(05): 129-137.

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链接本文: http://www.fzxb.org.cn/CN/10.13475/j.fzxb.20230102501

http://www.fzxb.org.cn/CN/Y2024/V45/I05/129

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试样编号	棉织物层数	水凝胶前体溶液的体积/mL	GO-Ag溶液体积/mL	APS质量/ mg
0^#	1	0	0	6
1^#	1	1.2	1	6
2^#	2	2.4	2	12
3^#	3	3.6	3	18
4^#	4	4.8	4	24
5^#	5	6.0	5	30

试样编号	断裂强力/N	断裂伸长率/%	断裂时间/s
0^#	213	103	59.6
1^#	260	129	73.4
2^#	320	132	95.5
3^#	170	188	111.7
4^#	342	159	106.5
5^#	298	130	74.7

试样编号	溶血率/%	细胞活性/%	毒性等级/级
1^#	2.77	75.66	1
2^#	3.67	87.09	1
3^#	4.27	101.24	0
4^#	6.06	84.06	1
5^#	6.77	81.53	1

聚氮异丙基丙烯酰胺抗菌水凝胶复合棉织物的制备及其性能

Preparation and properties of cotton fabric with poly(N-isopropylacrylamide) antibacterial hydrogel

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