静电纺纳米纤维纱线及其对细胞迁移和血管化的调控

RichHTML

PDF (PC)

静电纺纳米纤维纱线及其对细胞迁移和血管化的调控

Regulation of cell migration and vascularization using electrospun nanofiber yarns

静电纺纳米纤维纱线及其对细胞迁移和血管化的调控

Regulation of cell migration and vascularization using electrospun nanofiber yarns

摘要/Abstract

图/表 8

参考文献 34

Metrics

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 34

相关文章 15

Metrics

本文评价

推荐阅读 0

doi:10.13475/j.fzxb.20230904201

纺织学报 ›› 2024, Vol. 45 ›› Issue (12): 25-32.doi: 10.13475/j.fzxb.20230904201

王雅文¹^,², 刘娜², 王元非³, 吴桐¹^,²()

1.青岛大学纺织服装学院, 山东青岛 266071
2.青岛大学医学部, 山东青岛 266071
3.青岛大学附属青岛市口腔医院, 山东青岛 266001

收稿日期:2023-09-17 修回日期:2024-06-14 出版日期:2024-12-15 发布日期:2024-12-31
通讯作者: 吴桐(1989—),女,教授,博士。主要研究方向为生物医用材料研发与组织器官修复。E-mail:twu@qdu.edu.cn。
作者简介:王雅文(1993—),女,博士生。主要研究方向为生物医用材料。
基金资助:
山东省自然科学基金优秀青年基金项目(ZR2021YQ17)

WANG Yawen¹^,², LIU Na², WANG Yuanfei³, WU Tong¹^,²()

1. College of Textile & Clothing, Qingdao University, Qingdao, Shandong 266071, China
2. Medical College, Qingdao University, Qingdao, Shandong 266071, China
3. Qingdao Stomatological Hospital Affiliated to Qingdao University, Qingdao, Shandong 266001, China

Received:2023-09-17 Revised:2024-06-14 Published:2024-12-15 Online:2024-12-31

摘要：

为探究纳米纤维纱线网格支架培养脂肪干细胞收集的条件培养基对创面修复过程中成纤维细胞和内皮细胞迁移以及血管化的调控作用,通过静电纺丝技术和光焊技术制备了大、中、小3种不同尺寸的纳米纤维纱线网格支架,并将其与脂肪干细胞共培养提取条件培养基,用于培养内皮细胞和成纤维细胞。使用扫描电子显微镜、数码相机和红外热像仪观察网格支架的微观、宏观结构和焊接温度,并通过细胞迁移实验和体外成血管实验评价3种条件培养基对细胞行为的调控作用。结果表明:制备的3种纳米纤维纱线网格支架微观形貌一致,纳米纤维纱线直径为(257.69 ± 36.87) μm,焊接温度为(39.83 ± 3.07) ℃;通过支架提取的条件培养基可促进成纤维细胞和内皮细胞的迁移及血管化,其中通过小网格支架提取的条件培养基对细胞迁移的促进效果更为明显,通过小网格和中网格支架提取的条件培养基对血管化的促进效果更明显。

关键词: 支架材料, 静电纺丝, 创面修复, 内皮细胞, 成纤维细胞, 脂肪干细胞, 细胞迁移, 血管化

Abstract:

Objective Tissue engineering offers a promising therapeutic approach for chronic and acute skin injuries, primarily repairing and regenerating damaged tissues through artificial scaffolds. The migration of human skin fibroblasts (HSFs) and human umbilical vein endothelial cells (HUVECs) plays a crucial role in tissue repair and regeneration. Meanwhile, adipose stem cells (ADSCs) secrete various pro-angiogenic and anti-apoptotic factors essential for tissue repair and regeneration. Therefore, to investigate the effect of the conditioned medium of ADSCs on cell behaviors, three nanofiber yarn-based mesh scaffolds of different sizes were prepared and co-cultured with ADSCs, and the conditioned mediums obtained were co-cultured with HSFs and HUVECs to explore their regulatory effects on the migration and vascularization of these wound repair related cells.

Method The nanofiber yarn-based meshes with different sizes were prepared by light-welding and electrospinning, and the microstructure of different scaffolds was characterized by scanning electron microscopy (SEM) and digital photography, the welding temperatures of nanofiber yarn-based mesh scaffolds were measured by thermographic camera. ADSCs were cultured on scaffolds of different sizes to obtain different conditioned media, and HSFs and HUVECs were cultured with these different conditioned mediums. Cell viability was detected by CCK-8 kit, and cell morphology was observed by fluorescence microscopy.

Results SEM images and digital photographs showed the different sizes of nanofiber yarn-based mesh scaffolds and the uniform size of nanofiber yarns (257.69 ± 36.87) μm which was achieved at the welding temperature (39.83 ± 3.07) ℃. The viability and migration experiments of HSFs showed that the different conditioned mediums of nanofiber yarn-based mesh scaffolds had little effect on cell viability, but their biocompatibilities were improved over that of the control group. The healing rate of HSFs after scratches of small nanofiber yarn-based mesh scaffolds (SNS) and medium nanofiber yarn-based mesh scaffolds (MNS) was better than that of large nanofiber yarn-based mesh scaffolds (LMN) and control group. There was no significant difference in the effect of 3 different conditioned media on the viability of HUVECs among all groups, SNS group had better effect on the migration of HUVECs and SNS group and MNS group promoted the angiogenesis of HUVECs.

Conclusion The conditioned medium obtained after co-culturing ADSCs with nanofiber yarn meshes could effectively promote in vitro migration and angiogenesis of HSFs and HUVECS. Among them, the SNS scaffold was more effective in regulating cell behavior. The modulation of wound healing-related cell behavior utilizing nanofibrous scaffolds cultured with stem cell-collecting conditioned media is expected to be used in wound healing-related applications, providing new ideas for tissue regeneration and repair.

Key words: scaffold material, electrospinning, wound repair, human umbilical vein endothelial cell, human skin fibroblast, adipose stem cell, cell migration, vascularization

中图分类号:

TQ342.87

王雅文, 刘娜, 王元非, 吴桐. 静电纺纳米纤维纱线及其对细胞迁移和血管化的调控[J]. 纺织学报, 2024, 45(12): 25-32.

WANG Yawen, LIU Na, WANG Yuanfei, WU Tong. Regulation of cell migration and vascularization using electrospun nanofiber yarns[J]. Journal of Textile Research, 2024, 45(12): 25-32.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://www.fzxb.org.cn/CN/10.13475/j.fzxb.20230904201

http://www.fzxb.org.cn/CN/Y2024/V45/I12/25

图1

图2

表1

图3

图4

表2

图5

表3

[1]	LI J, YU F, CHEN G, et al. Moist-Retaining, Self-recoverable, bioadhesive, and transparent in situ forming hydrogels to accelerate wound healing[J]. ACS Applied Materials & Interfaces, 2020, 12(2): 2023-2038.
[2]	PEDRAM RAD Z, MOKHTARI J, ABBASI M. Fabrication and characterization of PCL/zein/gum arabic electrospun nanocomposite scaffold for skin tissue engineering[J]. Materials Science and Engineering: C, 2018, 93: 356-366.
[3]	王曙东, 马倩, 王可, 等. 3D生物打印制备组织工程支架的研究进展[J]. 纺织学报, 2023, 44(3): 210-220.
	WANG Shudong, MA Qian, WANG Ke, et al. Research progress in tissue engineering scaffolds by 3D bioprinting[J]. Journal of Textile Research, 2023, 44(3): 210-220.
[4]	AN Y, LIN S, TAN X, et al. Exosomes from adipose-derived stem cells and application to skin wound hea-ling[J]. Cell Proliferation, 2021. DOI: 10.1111/jocd.13215.
[5]	GIZAW M, FAGLIE A, PIEPER M, et al. The role of electrospun fiber scaffolds in stem cell therapy for skin tissue regeneration[J]. Med One, 2019. DOI: 10.20900/mo.20190002.
[6]	HAO Z, QI W, SUN J, et al. Review: research progress of adipose-derived stem cells in the treatment of chronic wounds[J]. Frontiers in Chemistry, 2023. DOI: 10.3389/fchem.2023.1094693.
[7]	SCHNEIDER I, CALCAGNI M, BUSCHMANN J. Adipose-derived stem cells applied in skin diseases, wound healing and skin defects: a review[J]. Cytotherapy, 2023, 25(2): 105-119.
[8]	BORGESE M, BARONE L, ROSSI F, et al. Effect of nanostructured scaffold on human adipose-derived stem cells: outcome of in vitro experiments[J]. Nanomaterials, 2020. DOI: 10.3390/nano10091822.
[9]	LIN L, XU Y, LI Y, et al. Nanofibrous Wharton's jelly scaffold in combination with adipose-derived stem cells for cartilage engineering[J]. Materials & Design, 2020. DOI: 10.1016/j.matdes.2019.108216.
[10]	NING X, LIU N, SUN T, et al. Promotion of adipose stem cell transplantation using GelMA hydrogel reinforced by PLCL/ADM short nanofibers[J]. Biomedical Materials, 2023. DOI: 10.1088/1748-605x/acf551.
[11]	HUANG W, XIAO Y, SHI X. Construction of electrospun organic/inorganic hybrid nanofibers for drug delivery and tissue engineering applications[J]. Advanced Fiber Materials, 2019, 1(1): 32-45.
[12]	DONG Y, ZHENG Y, ZHANG K, et al. Electrospun nanofibrous materials for wound healing[J]. Advanced Fiber Materials, 2020, 2(4): 212-227.
[13]	NULTY J, FREEMAN F E, BROWE D C, et al. 3D bioprinting of prevascularised implants for the repair of critically-sized bone defects[J]. Acta Biomaterialia, 2021, 126: 154-169.
[14]	BACAKOVA L, ZARUBOVA J, TRAVNICKOVA M, et al. Stem cells: their source, potency and use in regenerative therapies with focus on adipose-derived stem cells - a review[J]. Biotechnology Advances, 2018, 36(4): 1111-1126.
[15]	YAO Q, COSME J G L, XU T, et al. Three dimensional electrospun PCL/PLA blend nanofibrous scaffolds with significantly improved stem cells osteogenic differentiation and cranial bone forma-tion[J]. Biomaterials, 2017, 115: 115-127.
[16]	CHEN S, LI R, LI X, et al. Electrospinning: An enabling nanotechnology platform for drug delivery and regenerative medicine[J]. Advanced Drug Delivery Reviews, 2018, 132: 188-213.
[17]	付征, 穆齐峰, 张青松, 等. 胶体静电纺微纳米纤维的研究进展[J]. 纺织学报, 2023, 44(10): 196-204.
	FU Zheng, MU Qifeng, ZHANG Qingsong, et al. Research progress in colloidal electrospun micro/nano fibers[J]. Journal of Textile Research, 2023, 44(10): 196-204.
[18]	XIE J, MACEWAN M R, LI X, et al. Neurite outgrowth on nanofiber scaffolds with different orders, structures, and surface properties[J]. ACS Nano, 2009, 3(5): 1151-1159.
[19]	XUE J, WU T, DAI Y, et al. Electrospinning and electrospun nanofibers: methods, materials, and applications[J]. Chemical Reviews, 2019, 119(8): 5298-5415.
[20]	HEIDARI M, BAHRAMI H, RANJBAR-MOHAMMADI M. Fabrication, optimization and characterization of electrospun poly(caprolactone)/gelatin/graphene nanofibrous mats[J]. Materials Science and Engineering: C, 2017, 78: 218-229.
[21]	贾姣, 郑作保, 吴昊, 等. 静电纺聚合物复合金属有机框架功能纳米纤维膜的研究进展[J]. 纺织学报, 2023, 44(6): 215-224.
	JIA Jiao, ZHENG Zuobao, WU Hao, et al. Research progress in electrospinning functional nanofibers with metal-organic framework[J]. Journal of Textile Research, 2023, 44(6): 215-224.
[22]	TASKIN M B, AHMAD T, WISTLICH L, et al. Bioactive electrospun fibers: fabrication strategies and a critical review of surface-sensitive characterization and quantification[J]. Chemical Reviews, 2021, 121(18): 11194-11237.
[23]	XUE J, PISIGNANO D, XIA Y. Maneuvering the migration and differentiation of stem cells with electrospun nanofibers[J]. Advanced Science, 2020, 7(15): 2000735.
[24]	LIANG R, ZHAO J, LI B, et al. Implantable and degradable antioxidant poly(ε-caprolactone)-lignin nanofiber membrane for effective osteoarthritis treat-ment[J]. Biomaterials, 2020. DOI: 10.1016/j.biomaterials.2019.119601.
[25]	SAWADKAR P, MOHANAKRISHNAN J, RAJASEKAR P, et al. A synergistic relationship between polycaprolactone and natural polymers enhances the physical properties and biological activity of sca-ffolds[J]. ACS Applied Materials & Interfaces, 2020, 12(12): 13587-13597.
[26]	FENG Z, ZHANG X, LIU N, et al. Promotion of neurite outgrowth and extension using injectable welded nanofibers[J]. Chemical Research in Chinese Universities, 2021, 37(3): 522-527.
[27]	WU T, XUE J, XIA Y. Engraving the surface of electrospun microfibers with nanoscale grooves promotes the outgrowth of neurites and the migration of schwann cells[J]. Angewandte Chemie International Edition, 2020, 59(36): 15626-15632.
[28]	LIU Y, ZHANG X, WANG Y, et al. Promoting neurite outgrowth and neural stem cell migration using aligned nanofibers decorated with protrusions and galectin-1 coating[J]. Chemical Communications, 2023, 59(72): 10753-10756.
[29]	KALIRAJAN C, BEHERA H, SELVARAJ V, et al. In vitro probing of oxidized inulin cross-linked collagen-ZrO₂ hybrid scaffolds for tissue engineering applica-tions[J]. Carbohydrate Polymers, 2022. DOI: 10.1016/j.carbpol.2022.119458.
[30]	CHEN X, ZHANG L, CHAI W, et al. Hypoxic microenvironment reconstruction with synergistic biofunctional ions promotes diabetic wound healing[J]. Advanced Healthcare Materials, 2023. DOI: 10.1002/adhm.202301984.
[31]	DENG S, LEI T, CHEN H, et al. Metformin pre-treatment of stem cells from human exfoliated deciduous teeth promotes migration and angiogenesis of human umbilical vein endothelial cells for tissue enginee-ring[J]. Cytotherapy, 2022, 24(11): 1095-1104.
[32]	WU T, LI H, XUE J, et al. Photothermal welding, melting, and patterned expansion of nonwoven mats of polymer nanofibers for biomedical and printing applications[J]. Angewandte Chemie International Edition, 2019, 58(46): 16416-16421.
[33]	CHOI J K, JANG J H, JANG W H, et al. The effect of epidermal growth factor (EGF) conjugated with low-molecular-weight protamine (LMWP) on wound healing of the skin[J]. Biomaterials, 2012, 33(33): 8579-8590.
[34]	LIU Y, ZHU Z, PEI X, et al. ZIF-8-modified multifunctional bone-adhesive hydrogels promoting angiogenesis and osteogenesis for bone regenera-tion[J]. ACS Applied Materials & Interfaces, 2020, 12(33): 36978-36995.

Viewed

Full text

From	Others	local

Times	12	24
Rate	33%	67%

Abstract

138

Just accepted	Online first	Issue

0	0	138

From	Others	local

Times	102	36
Rate	74%	26%

Cited

Web of Science	Crossref	ScienceDirect	Search for Citations in Google Scholar >>


This page requires you have already subscribed to WoS.

Shared

Discussed

试样名称	HSFs 3 d时吸光度/nm	HSFs 24 h时迁移率/%
对照组	0.654 8 ± 0.041 9	10.19 ± 0.25
LNS	0.834 2 ± 0.066 9	47.07 ± 2.83
MNS	0.879 6 ± 0.044 2	49.84 ± 1.89
SNS	0.868 0 ± 0.027 7	57.21 ± 5.20

试样名称	HUVECs 3 d时吸光度/nm	HUVECs 24 h时迁移率/%
对照组	0.850 0 ± 0.079 7	26.05 ± 4.68
LNS	0.823 4 ± 0.074 5	48.40 ± 4.56
MNS	0.844 1 ± 0.015 8	47.67 ± 4.27
SNS	0.864 9 ± 0.034 8	65.55 ± 3.00

试样名称	HUVECs 6 h后成管长度/μm	HUVECs 6 h后节点数量/个
对照组	22.46 ± 1.15	983.40 ± 57.84
LNS	25.67 ± 2.63	1 081.60 ± 162.81
MNS	29.30 ± 2.00	1 289.80 ± 103.93
SNS	29.61 ± 2.41	1 233.20 ± 74.88

Just accepted

Online first

Just accepted

Online first

[1]	雷福旺, 冯其, 侯奥菡, 赵振鸿, 谭佳兆, 赵景, 王先锋. 聚偏氟乙烯-聚丙烯腈/SiO₂单向导湿纤维膜的制备及其性能[J]. 纺织学报, 2024, 45(12): 1-8.
[2]	刘霞, 吴改红, 闫子豪, 王彩柳. 智能相变调温聚乳酸纤维膜的制备及其性能[J]. 纺织学报, 2024, 45(12): 18-24.
[3]	卢海龙, 于影, 左雨欣, 王浩然, 陈洪立, 汝欣. 取向增强抗CO₂腐蚀纤维薄膜的制备及其性能[J]. 纺织学报, 2024, 45(12): 33-40.
[4]	刘健, 董守骏, 王程皓, 刘泳汝, 潘山山, 尹兆松. 花瓣状多尖端静电纺丝喷头的电场模拟及优化[J]. 纺织学报, 2024, 45(10): 191-199.
[5]	张佃平, 王昊, 林文峰, 王振秋. 多喷头纺丝装置的仿真与设计[J]. 纺织学报, 2024, 45(10): 200-207.
[6]	蔺志浩, 房磊, 贾娇娇, 扈延龄, 房宽峻. 负载生长因子的微纳米纤维创面敷料的制备与应用研究进展[J]. 纺织学报, 2024, 45(09): 244-251.
[7]	王浩然, 于影, 左雨欣, 顾志清, 卢海龙, 陈洪立, 柯俊. 聚乙烯亚胺/聚丙烯腈复合纤维薄膜的制备及其功能化应用[J]. 纺织学报, 2024, 45(09): 26-32.
[8]	王清鹏, 张海艳, 王雨婷, 张涛, 赵燕. 聚环氧乙烷/Al₂O₃被动辐射降温膜的制备及其性能[J]. 纺织学报, 2024, 45(09): 33-41.
[9]	钱洋, 张璐, 李晨阳, 王荣武. 静电纺海藻酸钠复合纳米纤维膜制备及其性能[J]. 纺织学报, 2024, 45(08): 18-25.
[10]	刘嘉炜, 季东晓, 覃小红. 空气过滤用静电纺纳米纤维材料研究进展[J]. 纺织学报, 2024, 45(08): 35-43.
[11]	刘德龙, 王红霞, 林童. 气流辅助的静电纺丝技术研究进展[J]. 纺织学报, 2024, 45(08): 44-53.
[12]	杨硕, 赵朋举, 程春祖, 李晨暘, 程博闻. 非对称润湿性纤维复合膜的制备及其油水分离性能[J]. 纺织学报, 2024, 45(08): 10-17.
[13]	王永政, 黄林涛, 宋付权. 石油沥青/聚丙烯腈静电纺碳纳米纤维的制备工艺优化及其性能[J]. 纺织学报, 2024, 45(08): 107-115.
[14]	闫迪, 王雪芳, 谭文萍, 高国金, 明津法, 宁新. 富咪唑型多孔左旋聚乳酸纳米纤维膜制备及其双重净水性能[J]. 纺织学报, 2024, 45(08): 116-126.
[15]	陈灿, 拖晓航, 王迎. 取向聚氨酯纳米纤维膜卷纱的制备及其力学性能[J]. 纺织学报, 2024, 45(08): 134-141.