普鲁士蓝涂层非织造材料在细菌检测中的应用

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普鲁士蓝涂层非织造材料在细菌检测中的应用

Application of Prussian blue coated nonwoven materials in bacterial detection

普鲁士蓝涂层非织造材料在细菌检测中的应用

Application of Prussian blue coated nonwoven materials in bacterial detection

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doi:10.13475/j.fzxb.20231104301

纺织学报 ›› 2024, Vol. 45 ›› Issue (12): 166-171.doi: 10.13475/j.fzxb.20231104301

夏梦¹, 成悦¹, 刘蓉¹^,², 李大伟¹^,², 付译鋆¹^,²()

1.南通大学纺织服装学院, 江苏南通 226019
2.安全防护用特种纤维复合材料研发国家地方联合工程研究中心, 江苏南通 226019

收稿日期:2023-11-20 修回日期:2024-04-15 出版日期:2024-12-15 发布日期:2024-12-31
通讯作者: 付译鋆(1989—),女,副教授,博士。主要研究方向为生物医用纺织材料。E-mail:fuyj@ntu.edu.cn。
作者简介:夏梦(1999—),女,硕士生。主要研究方向为生物医用纺织材料。
基金资助:
江苏省高等学校基础科学(自然科学)研究重大项目(24KJA540002);江苏省青年科技人才托举工程项目(JSTJ-2024-045)

XIA Meng¹, CHENG Yue¹, LIU Rong¹^,², LI Dawei¹^,², FU Yijun¹^,²()

1. School of Textile and Clothing, Nantong University, Nantong, Jiangsu 226019, China
2. National & Local Joint Engineering Research Center of Technical Fiber Composites for Safety and Health, Nantong, Jiangsu 226019, China

Received:2023-11-20 Revised:2024-04-15 Published:2024-12-15 Online:2024-12-31

摘要：

针对传统细菌检测方法操作步骤复杂、检测时间长、成本高等问题,研制了具有细菌检测功能的医疗卫生用非织造材料。首先制备了普鲁士蓝纳米颗粒(PB NPs),然后利用声化学涂层技术对聚丙烯(PP)纺黏非织造材料进行涂层整理,制得可检测细菌的功能非织造材料,并对其形貌结构、化学成分和细菌检测性能进行分析研究。结果表明:PB NPs形貌呈现明显的立方体结构;声化学涂层后的非织造材料(PP/PB)在与金黄色葡萄球菌和大肠杆菌培养24 h后,可观察到明显的颜色变化,表明其具有优异的细菌检测功能。

关键词: 普鲁士蓝, 声化学涂层, 非织造材料, 细菌检测

Abstract:

Objective The breeding and spread of bacteria on medical and hygienic materials are a major threat to human health. Therefore, it is of great significance to develop bacteria detective textile materials with high efficiency and performance. Prussian blue (PB) is a coordination compound with a redox potential high enough to react with proteins and mediators of the bacterial electron transport chain. Besides, it has a high extinction molar coefficient, distinct color change after reduction, which can be used as an indicator of bacterial reduction metabolism. Polypropylene (PP) spunbonded nonwovens have good chemical stability and are not easy to chemically react with Prussian blue or other substances tested, and the large surface area and pores of PP spunbonded nonwovens contribute to the loading of Prussian blue nanoparticles (PB NPs). In this paper, PP spunbonded nonwoven and PB NPs were employed as substrate and coating material respectively to prepare functional nonwoven with bacterial detection function by acoustic chemical coating technology.

Method PB NPs were firstly prepared by using polyethylpyrrolidone and potassium ferricyanide as raw materials. Then, polyvinyl alcohol modified PP nonwoven (PP/PVA) was prepared by hydrophilic modification. As the last step, PB NPs were combined onto PP/PVA nonwoven by means of acoustic chemical coating technology to obtain PP/PB nonwoven. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) were used to characterize the morphology, chemical and crystalline structures of PB NPs, PP, PP/PVA and PP/PB nonwovens. Especially, the bacterial detection performance of PP/PB nonwoven was evaluated by oscillating method.

Results It was evident from the SEM image that the prepared PB NPs had a regular cubic structure with uniform particle size distribution. The fibers of pure PP nonwoven were arranged dandomly and the fiber surface was smooth and free of impurities, while obvious particles and network adhesion were found on the surface of PP/PVA nonwoven and PP/PB nonwoven, respectively. The infrared spectra of different samples showed that PP/PB nonwoven retained the characteristic absorption peaks of PP/PVA and PB NPs, indicating that PB NPs was successfully loaded on PP nonwoven. XRD patterns of different samples show that PP/PB nonwoven demopnstrated diffraction peaks of PP (2θ=14.18°, 17.05°, 18.64°, 21.36° and 25.37°) and PB NPs (2θ=35.42°, 39.76°, 43.74°, 50.95°, 54.29° and 57.49°), implying that PB NPs were successfully loaded onto PP nonwoven by acoustic chemical coating technology. Finally, the bacterial detection performance of PP/PB nonwoven was investigated. With the increase of the concentration of PB NPs solution, the color of the solution gradually deepened. After 24 h of incubation in the bacterial solution containing Staphylococcus aureus and Escherichia coli, the color of the samples changed from blue to white, indicating that PP/PB nonwoven fabric could detect these bacteria through its color development reaction. Therefore, it is possible to applyPB NPs to medical and hygienic materials such as surgical gowns and masks to achieve the detection and early warning of bacteria through the color reaction, so as to effectively avoid adverse effects caused by bacteria.

Conclusion The PP/PB nonwoven fabric with PB NPS coating were prepared by acoustic chemical coating technology, which has excellent bacterial detection performance. After inoculation with Staphylococcus aureus and Escherichia coli, the color of PP/PB nonwoven fabric changed from blue to white. It can be applied to medical care products such as surgical gowns and masks to realize the detection and early warning of bacteria through color reaction, so as to effectively avoid the adverse effects of bacteria.

Key words: Prussian blue, acoustochemical coating, nonwoven materials, bacteria detection

中图分类号:

TS101.8

夏梦, 成悦, 刘蓉, 李大伟, 付译鋆. 普鲁士蓝涂层非织造材料在细菌检测中的应用[J]. 纺织学报, 2024, 45(12): 166-171.

XIA Meng, CHENG Yue, LIU Rong, LI Dawei, FU Yijun. Application of Prussian blue coated nonwoven materials in bacterial detection[J]. Journal of Textile Research, 2024, 45(12): 166-171.

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链接本文: http://www.fzxb.org.cn/CN/10.13475/j.fzxb.20231104301

http://www.fzxb.org.cn/CN/Y2024/V45/I12/166

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