Abstract: Non-woven web of uniformly mixed cotton short staple fiber with biodegradable poly(hydroxybutyrate-co-hydroxyvalerate)(PHBV) was manufactured by wet laying method with cotton linters and wastes as raw materials.Utilizing the good thermo-plasticity of PHBV,thermo-bonded non-woven fabric was developed,Which was complete biodegradable and suitable for medical use.Research indicated that this environmentally protective non-woven fabric for medical use has good properties.