Abstract: In order to realize the regeneration and application of waste wool、improve the recovery rate of macromolecular keratin and extend the application fields of keratin resources, NaHSO3/LiBr/SDS dissolution system was used to deal with waste wool and study on preparation of non-toxic、high dissolving rate and high molecular weight of stable keratin solution. .By means of the single factor contrast tests of component use levels、pH value、reaction temperature and reaction time, the optimum reaction conditions were obtained through investigating the texting indexes including wool dissolution rate and large molecular weight keratin extraction rate. Results showed that: the keratin solution could be obtained at 94 % wool dissolution rate and 50.2% keratin extraction rate under the optimal condition when NaHSO3 concentration was 0.5 mol/L, LiBr concentration was 0.1 mol/L, SDS concentration was 0.02 mol/L, reaction temperature was 90℃,reaction time was 4 hours , pH value was 12. Although the reduction system without the addition of LiBr remained at a high dissolution rate, the low extraction rate only presented 30%. IR spectra showed that the extracted keratin from solution system with the addition of LiBr was dominated a-helical conformation. It can be used in textile materials and development of new functional fibers.