Abstract: The fiber orientation is an important structure index of nonwoven. In this paper, through programming with MATLAB, we simulated various fiber orientations of nonwoven, such as 45°, 90°, 150°. Then, using image analysis technology and the Two-Dimensional Discrete Fourier Transform (2DDFT), fiber orientation distributions of nonwoven were obtained. What’s more, effects of threshold methods and morphological processing on measuring fiber orientation were discussed and analyzed. It is valuable for quantitative expressing structural feature of nonwoven.