Abstract: A method for determination of phenolic environmental hormones such as bisphenol A , 4-nonylphenol and octylphenol in cashmere fabrics by high performance liquid chromatography with diode array detector and fluorescence detector (HPLC-DAD-FLD) was established. After cashmere fabrics were extracted by Soxhlet Extraction, the extracts were separated and eluted on a fast chromatographic column ,Shim-pack XR-ODSⅡ（100 mm×2.0 mm i.d., 2.2 μm）by using the mobile phase consisting of CH3OH+CH3CN+H2O (30+50+20, V/V/V) . Detection was performed by DAD at 225 nm and FLD at λex 228 nm, λem 305 nm using extenal standard method. The result of experiment indicates that calibration curves of 3 phenolic environmental hormones were linear within the range of 0.01~1.0 mg/kg for FLD, the limits of quantification were found to be 0.01 mg/kg. Calibration curves were linear within the range of 0.05~10.0 mg/kg for UV, the limits of quantification were 0.05 mg/kg for them. The extraction recoveries were between 82.0~102.0% and the average recovery was 89.2%, the RSD was 1.8~7.5%. This method is found to be simple, sensitive, accurate, little interferential for the determination of bisphenol A , 4- nonylphenol and octylphenol residue in cashmere fabrics.