Abstract: In order to save water in textile dyeing, the non-ionic reverse micelles were prepared with a non-ionic surfactant Triton X-100 and isooctane by injecting small amount of Acid Black 234 aqueous solution. And then the adsorption and dyeing properties of the dye onto wool in the non-ionic reverse micelle and bulk water were studied, respectively, based on the adsorption mechanism between them examined. The results indicated that Acid Black 234 prefer to exhibit the monomolecular state in the reverse micelle. Low pH values would increase the adsorption amount of the dye onto wool. The adsorption of the dye onto wool showed better agreement with Langmuir isotherm equation and Lagergren second order equation. Acid Black 234 had lower the adsorption amount and diffusion active energy in the reverse micelle than bulk water, but its diffusion coefficient exhibited reverse trend. Little difference in the color characteristics of the dyed wool with Acid Black 234 between in the reverse micelle and bulk water was found. However, a slight decrease in color strength of the dyed wool in the reverse micelle was observed, which may be mainly due to the high diffusion coefficient.