Abstract: To research the effect of histological structure on the light-proofness, polyester yarn were selected as the materials and three types of fabrics about 25 pieces with double-layer construction were woven. Fabric light-proof was tested and analyzed. This research explores pattern of organization, organization cycle numbers, binding points distribution, backing organization to get the regularity of light-proofness. It concludes as follows. As the binding points on unit area are decreased, the light-proofness is strengthened. With certain fabric density and controlled floats, the relation between organization cycle numbers and average light-proof rate presents a positive correlation. When the number is more than eight, the effects are basically consistent. Regarding the fabrics with same organization cycle numbers, the effects of light-proofness can be arranged as, plain weave < twill weave < satin weave. Besides, the binding points should be evenly distributed to assure the flatness of two layers. Satin weave is the better choice of backing weave.