活性艳橙K-7R结合丝素肽的分子光谱学特征

纺织学报 ›› 2014, Vol. 35 ›› Issue (3): 81-0.

活性艳橙K-7R结合丝素肽的分子光谱学特征

马明明杨家彪同帜苏新科

西安工程大学环境与化工学院

收稿日期:2013-01-28 修回日期:2013-09-03 出版日期:2014-03-15 发布日期:2014-03-11
通讯作者: 马明明 E-mail:18791985070@163.com
基金资助:
陕西省科学计划项目；西安工程大学自然科学基金资助项目；西安工程大学博士启动基金资助项目

Molecular spectroscopic characteristics of binding between silk peptide and Reactive Orange K-7R

Received:2013-01-28 Revised:2013-09-03 Online:2014-03-15 Published:2014-03-11

摘要/Abstract

摘要： 本文通过紫外可见、荧光光谱、同步荧光、瑞利散射共振光谱等分子光谱技术研究发现，活性艳橙K-7R(RK-7R)与丝素肽结合为1:1、作用距离为3.46nm的非荧光性复合物。活性艳橙K-7R印染体系中可能存在的阳离子表面活性剂CTAB、3种离子液体（溴化1-乙基-2，3-二甲基咪唑、1-乙基-2，3-甲基咪唑双三氟甲磺酰亚胺盐、氯化羟乙基三甲基胺），3种金属离子（Ca2+、Pb2+、Ba2+）都增强了丝素肽与活性艳橙K-7R的结合；而非离子型表面活性剂曲拉通X-100以及Cu2+均削弱二者结合，阴离子表面活性剂十二烷基磺酸钠对二者结合不影响。

关键词: 丝素肽, 活性艳橙K-7R, 分子光谱, 结合作用

Abstract: To investigate the interaction between silk peptide and reactive orange K-7R(RK-7R), multiple spectroscopy (Ultraviolet visible, fluorescence spectroscopy, synchronous fluorescence, Rayleigh resonance spectrum have been used. The results showed that a non fluorescence complex was formed between silk peptide and RK-7R. The binding distance and binding ratio were calculated as 3.46nm and 1:1, respectively. The binding constants increased when ionic liquids including chloride hydroxyethyl trimethyl amine, bromide 1-ethyl-2 ,3–dimethyl imidazolium, 1-ethyl-2,3-methyl imidazolium bis-[trifluoromethyl-sulfonyl] imide, metal ions (Ca2 +, Pb2 +, Ba2 +), and surfactants CTAB were added into SP- RK-7R Ssystem ,while decreased when Cu2+ and Triton X-100 were in the presence.. Sodium dodecyl sulfate had no effects on the system.

Key words: silk peptide, Reactive Orange K-7R, specteosopy, interaction

马明明杨家彪同帜苏新科. 活性艳橙K-7R结合丝素肽的分子光谱学特征[J]. 纺织学报, 2014, 35(3): 81-0.

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