纺织学报 ›› 2019, Vol. 40 ›› Issue (02): 20-25.doi: 10.13475/j.fzxb.20181006406

• 纤维材料 • 上一篇    下一篇

紫外-可见分光光度法在定量监测2,3,5-三碘苯甲酸缓释中的应用

刘来俊1,2, 徐海燕1,2, 赵帆1,2, 王富军1,2(), 王璐1,2   

  1. 1.东华大学 纺织学院, 上海 201620
    2.东华大学 纺织面料技术教育部重点实验室, 上海 201620
  • 收稿日期:2018-10-31 修回日期:2018-11-16 出版日期:2019-02-15 发布日期:2019-02-01
  • 通讯作者: 王富军
  • 作者简介:刘来俊(1992—),男,博士生。主要研究方向为生物医用骨修复纺织材料。
  • 基金资助:
    高等学校学科创新引智计划资助项目(b07024);上海市科技支撑计划资助项目(16441903803);上海市科技支撑计划资助项目(18441902600)

Application of UV-Vis spectrophotometry in quantitative monitoring sustained release of 2,3,5-triiodobenzoic acid

LIU Laijun1,2, XU Haiyan1,2, ZHAO Fan1,2, WANG Fujun1,2(), WANG Lu1,2   

  1. 1. College of Textiles, Donghua University, Shanghai 201620, China
    2. Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620,China
  • Received:2018-10-31 Revised:2018-11-16 Online:2019-02-15 Published:2019-02-01
  • Contact: WANG Fujun

RichHTML

6

PDF (PC)

79

摘要：

为研究显影剂2,3,5-三碘苯甲酸(TIBA)在显影纤维聚对二氧环己酮(PPDO)降解液中的缓释规律,采用紫外可见分光光度法探究了TIBA在不同体积比的磷酸盐缓冲溶液(PBS)和乙醇共混降解液(VPBS:V乙醇分别为1:3、1:1和3:1)中的紫外-可见分光光谱和回归曲线,根据回归曲线,测定降解液的吸光度值及计算PPDO纤维在降解过程中显影剂TIBA的释放量,借助药物缓释理论模型进行拟合,并分析相应的释放规律。结果表明:在3种共混溶液中,均能得到回归关系明显的线性方程,考虑到乙醇用量,选择PBS与乙醇的体积比为3:1的溶液作为标准液,并且溶液在37 ℃的环境中静置8 d后仍能保持一定的稳定性;模型拟合结果表明TIBA的释放规律符合一级释放的函数模型。

关键词: 紫外-可见分光光度法, 显影纤维, 纤维降解, 2,3,5-三碘苯甲酸, 释放模型

Abstract:

In order to study the sustained release rules of 2,3,5-triiodobenzoic acid (TIBA) in degradation solution of radiopaque poly-p-dioxanone (PPDO) filaments, the UV spectrums and regression curves of TIBA in different volume ratio blend degradation solutions of phosphate buffered saline (PBS) and ethanol (VPBS:Vethanol were 1:3,1:1 and 3:1) were explored by UV-Vis Spectrophotometry. According to regression curve, the release amount of TIBA in the degradation period was measured and calculated and then fitted with a drug release models and the corresponding release rules were analyzed. The results show that ideal spectrums and regression curves could be obtained in three kinds of blend solution. Considering the amount of ethanol used, the volume ratio of 3:1 was selected as the standard solution and it can still maintain stability after placing in an environment of 37 ℃ for 8 d. The results of model fit show that the release rule of TIBA is in consistence with the first-order release model.

Key words: ultraviolet-visible spectrophotometry, developing fiber, fiber degradation, 2,3,5-triiodobenzoic acid, release models

中图分类号: 

  • TS101

图1

降解液收集流程示意图"

图2

TIBA在R1溶液中的光谱图和回归分析"

图3

TIBA在R2溶液中的光谱图和回归分析"

图4

TIBA在R3溶液中的光谱图和回归分析"

图5

8 d后TIBA在R3溶液中的光谱图和回归分析"

表1

药物释放常规模型"

释放模型 函数模型 释放机制
零级释放 Mt/M0=kt 恒速释放
一级释放 ln(1-Mt/ M0)=-kt 一级释放
Peppas-Korsmeyer方程 Mt/M0=ktn 扩散和溶蚀
Higuchi方程 Mt/M0=kt1/2 Fick扩散

图6

S1、S2和S3 中TIBA的累积释放率"

表2

S1、S2和S3中TIBA缓释拟合参数"

释放模型 释放动力学系数k R2
S1 S2 S3 S1 S2 S3
零级释放 0.044 9 0.043 3 0.044 7 0.687 0.837 0.802
一级释放 0.506 2 0.168 1 0.216 0 0.958 0.985 0.995
Peppas-Korsmeyer方程 0.588 1 0.316 0 0.362 9 0.925 0.958 0.925
Higuchi方程 0.226 3 0.208 3 0.217 3 0.432 0.912 0.848

图7

S2中TIBA的累积释放率-时间拟合结果"

[1] QIU Z C, ZHANG J J, HUANG C L, et al. The influence of the surface character of the clays on the properties of poly(p-dioxanone)/fibrous clay nanocomposites[J]. Journal of Applied Polymer Science, 2012,125:247-259.
[2] ZHAO F, XUE W, WANG F J, et al. A new approach to improve the local compressive properties of PPDO self-expandable stent[J]. Journal of the Mechanical Behavior of Biomedical Materials, 2017,68:318-326.
doi: 10.1016/j.jmbbm.2017.02.015 pmid: 28237687
[3] ZHAO Y Q, DING S D, YUAN Y, et al. Enhanced degradation stability of poly(p-dioxanone) under different temperature and humidity with bis-(2,6-diisopropylphenyl) carbodiimide[J]. Journal of Applied Polymer Science, 2014,131(6):596-602.
[4] 李利发, 张井潇, 陈小波, 等. 三种不同缝合线对腹壁手术切口愈合质量影响的研究[J]. 中国普外基础与临床杂志, 2016,23(4):445-449.
LI Lifa, ZHANG Jingxiao, CHEN Xiaobo, et al. Study on the effect of three kinds of different suture on the healing quality of abdominal incision[J]. Chinese Journal of Bases and Clinics in General Surgery, 2016,23(4):445-449.
[5] ZHAO F, XUE W, WANG F J, et al. Composite self-expanding bioresorbable prototype stents with reinforced compression performance for congenital heart disease application: computational and experimental investigation[J]. Journal of the Mechanical Behavior of Biomedical Materials, 2018,84:126-134.
doi: 10.1016/j.jmbbm.2018.05.009 pmid: 29775814
[6] TANG H, XU Z, QIN X, et al. Chest wall reconstruction in a canine model using polydioxanone mesh, demineralized bone matrix and bone marrow stromal cells[J]. Biomaterials, 2009,30(19):3224-3233.
doi: 10.1016/j.biomaterials.2009.02.007 pmid: 19233465
[7] SINGHANA B, CHEN A, SLATTERY P, et al. Infusion of iodine-based contrast agents into poly(p-dioxanone) as a radiopaque resorbable IVC filter[J]. Journal of Materials Science: Materials in Medicine, 2015,26(3):124.
pmid: 25690619
[8] 曲乐, 曹江, 黄新苗. 可降解聚对二氧环己酮材料的临床应用[J]. 中国组织工程研究与临床康复, 2011,15(3):527-530.
QU Le, CAO Jiang, HUANG Xinmiao. Clinical application of biodegradable polydioxanone[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011,15(3):527-530.
[9] SERIO P, FAINARDI V, LEONE R, et al. Tracheobronchial obstruction: follow-up study of 100 children treated with airway stenting[J]. European Journal of Cardio-Thoracic Surgery, 2014,45(4):100-109.
doi: 10.1093/ejcts/ezt284
[10] LEON C M, LEE B H, PREUL M, et al. Synjournal and characterization of radio-opaque thermosensitive poly N-isopropylacrylamide-2,2'-(ethylenedioxy)bis(ethylamine)-2,3,5-triiodobenzamide[J]. Polymer International, 2009,58(8):847-850.
doi: 10.1002/pi.2616
[11] STURZU A, VOGEL U, GHARABAGHI A, et al. Cell nucleus directed 2,3,5-triiodobenzoic acid conju-gates[J]. Medicinal Chemistry, 2009,5(4):385-391.
pmid: 19689397
[12] MARTYNENKO A, CHEN Y. Degradation kinetics of total anthocyanins and formation of polymeric color in blueberry hydrothermodynamic (HTD) processing[J]. Journal of Food Engineering, 2016,171(4):44-51.
doi: 10.1016/j.jfoodeng.2015.10.008
[13] 陈力, 刘砚韬, 黄亮, 等. 缓控释系统药物释放的数学模型研究进展[J]. 中国药业, 2008,17(11):1-4.
CHEN Li, LIU Yantao, HUANG Liang, et al. Research progress of mathematic model of sustained and controlled release system of drug release[J]. China Pharmaceuticals, 2008,17(11):1-4.
[14] TABANDEH H, MORTAZAVI S A. An investigation into the drug release from ibuprofen matrix tablets with ethylcellulose and some poly-acrylate polymers[J]. Pakistan Journal of Pharmaceutical Sciences, 2014,27(3):495-503.
pmid: 24811808
[15] KAFFASHI B, DAVOODI S, OLIAEI E. Poly(epsilon-caprolactone)/triclosan loaded polylactic acid nanoparticles composite: a long-term antibacterial bionanocomposite with sustained release[J]. International Journal of Pharmaceutics, 2016,508(1/2):10-21.
doi: 10.1016/j.ijpharm.2016.05.009
[16] KARAMI Z, REZAEIAN I, ZAHEDI P, et al. Preparation and performance evaluations of electrospun poly(epsilon-caprolactone), poly(lactic acid), and their hybrid (50/50) nanofibrous mats containing thymol as an herbal drug for effective wound healing[J]. Journal of Applied Polymer Science, 2013,129(2):756-766.
doi: 10.1002/app.38683
[17] 钟萌, 谭群友, 冯悦, 等. 阿奇霉素囊泡体外释放行为的初步研究[J]. 中国抗生素杂志, 2012,37(6):476-480.
ZHONG Meng, TAN Qunyou, FENG Yue, et al. Preliminary study on the release of azithromycin niosomes in vitro[J]. Chinese Journal of Antibiotics, 2012,37(6):476-480.
[1] 陈莹, 周爽, 韦恬静, 方浩霞, 李宇菲. 聚吡咯复合织物的软模板法制备及其性能[J]. 纺织学报, 2019, 40(12): 93-97.
[2] 魏艳红, 刘新金, 谢春萍, 苏旭中, 张钟唏. 聚酯长丝/棉复合纱斜纹织物的保形性及服用性能[J]. 纺织学报, 2019, 40(12): 39-44.
[3] 肖志涛, 郭永敏, 耿磊, 吴骏, 张芳, 王雯, 刘彦北. 基于超声相控阵的机织层合复合薄板试件内部缺陷检测方法[J]. 纺织学报, 2019, 40(11): 81-87.
[4] 杨恩惠, 邱华, 代文杰. 基于六边形网格结构的针织物三维建模[J]. 纺织学报, 2019, 40(11): 69-74.
[5] 吴莹, 占竹, 汪军. 基于通用字典的局部机织物纹理稳定表征[J]. 纺织学报, 2019, 40(11): 45-49.
[6] 贾高鹏, 宋小红, 李莹, 刘晓丹, 潘雪茹. 铜镍金属涂层机织物拉伸过程中电流的响应[J]. 纺织学报, 2019, 40(10): 68-72.
[7] 钱成, 刘燕卿, 刘新金, 谢春萍, 徐伯俊. 四罗拉集聚纺纱系统三维流场模拟与分析[J]. 纺织学报, 2019, 40(10): 56-61.
[8] 董科, 张玲, 范佳璇, 李梦婕, 梅琳, 肖学良. 织物电极监测心电信号与穿戴压力作用机制分析[J]. 纺织学报, 2019, 40(09): 75-82.
[9] 刘沐黎, 袁理, 杨亚莉, 刘军平, 程哲, 龚雪, 纪昌俊, 鄢煜尘. 基于混合色彩空间独立特征的色纺面料颜色表征模型[J]. 纺织学报, 2019, 40(09): 62-69.
[10] 韩健健, 胡勇杰, 胡敏专. 基于纳滤技术的质检萃取液脱色预处理方法[J]. 纺织学报, 2019, 40(09): 136-142.
[11] 邹梨花, 徐珍珍, 孙妍妍, 王太冉, 邱夷平. 氧化石墨烯/聚苯胺功能膜对棉织物电磁屏蔽性能的影响[J]. 纺织学报, 2019, 40(08): 109-116.
[12] 孙卫红, 阮棉奖, 邵铁锋, 梁曼. 基于机器视觉的生丝抱合性能检测方法[J]. 纺织学报, 2019, 40(08): 164-168.
[13] 林永佳, 杨董超, 张佩华, 顾岩. 再生丝素蛋白/脱细胞真皮基质共混纳米纤维膜的制备及其性能[J]. 纺织学报, 2019, 40(07): 13-18.
[14] 朱浩, 丁辉, 尚媛园, 邵珠宏. 多纹理分级融合的织物缺陷检测算法[J]. 纺织学报, 2019, 40(06): 117-124.
[15] 郑振荣, 智伟, 韩晨晨, 赵晓明, 裴晓园. 碳纤维织物在热流冲击下的热传递数值模拟[J]. 纺织学报, 2019, 40(06): 38-43.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备14006648号　 京公网安备11010502044800号
版权所有 © 2021 《纺织学报》编辑部
地址： 北京市朝阳区延静里中街3号主楼6层《纺织学报》杂志社　邮政编码：100025　
电话：010-65017711,65917740　传真：010-65016539　Email:fangzhixuebao@vip.126.com
本系统由北京玛格泰克科技发展有限公司设计开发