纺织学报 ›› 2020, Vol. 41 ›› Issue (09): 167-173.doi: 10.13475/j.fzxb.20200307807

• 专栏:生物医用纺织材料及其制品 • 上一篇    下一篇

聚己内酯/聚乙二醇大孔径纳米纤维膜的制备及其在组织工程支架中的应用

潘璐1, 程亭亭1, 徐岚1,2()   

  1. 1.苏州大学 纺织与服装工程学院, 江苏 苏州 215123
    2.现代丝绸国家工程实验室, 江苏 苏州 215123
  • 收稿日期:2020-03-30 修回日期:2020-06-04 出版日期:2020-09-15 发布日期:2020-09-25
  • 通讯作者: 徐岚
  • 作者简介:潘璐(1996—),女,硕士生。主要研究方向为纳米纤维的制备及其应用。
  • 基金资助:
    国家自然科学基金项目(11672198)

Preparation of polycaprolactone/polyethylene glycol nanofiber membranes with large pore sizes and its application for tissue engineering scaffold

PAN Lu1, CHENG Tingting1, XU Lan1,2()   

  1. 1. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China
    2. National Engineering Laboratory for Modern Silk, Suzhou, Jiangsu 215123, China
  • Received:2020-03-30 Revised:2020-06-04 Online:2020-09-15 Published:2020-09-25
  • Contact: XU Lan

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摘要：

为使细胞在静电纺纳米纤维支架上得到更佳的生长与黏附,采用改进的静电纺丝装置制备具有良好生物相容性的聚己内酯(PLC)/聚乙二醇(PEG)大孔径复合纳米纤维膜,探究纺丝溶液中溶质质量配比与溶液质量分数对纳米纤维膜形貌及性能的影响,确定最佳工艺参数;将最佳工艺条件下制备的纳米纤维膜初步应用于组织工程,并与传统静电纺丝装置制备的纤维膜进行细胞相容性对比分析。结果表明:当PLC和PEG的混纺质量比为80∶20,纺丝溶液质量分数为25%时,获得的PCL/PEG大孔径纳米纤维膜质量最好;与传统静电纺PCL/PEG纳米纤维膜相比,PCL/PEG大孔径纳米纤维膜更利于细胞的生长和增殖,更适合作为组织工程支架材料。

关键词: 静电纺丝, 大孔径纳米纤维膜, 组织工程支架, 聚己内酯, 聚乙二醇

Abstract:

In order to improve the growth and adhesion of cells on electrospun nanofiber scaffolds, a modified electrospinning (ES) device was used to prepare polycaprolactone (PLC)/polyethylene glycol (PEG) composite nanofiber membranes (CNFMs) with large pore sizes, aiming for good biocompatibility. The influences of the composition and mass fraction of spinning solution on the morphology and properties of the CNFMs were investigated, and the optimal spinning parameters were determined. The CNFMs obtained using the modified ES under the optimal conditions were taken as tissue engineering scaffold, and the cytocompatibility of the CNFMs was compared with that of the CNFMs prepared by the traditional ES. The results show that when the blend mass ratio of PLC and PEG is 80∶20 and the mass fraction of spinning solution is 25%, the quality of PCL/PEG CNFMs with large pore sizes is the best. Moreover, compared with the PCL/PEG CNFMs obtained by the traditional ES, the PCL/PEG CNFMs with large pore sizes supply a more conducive environment for the cell growth and proliferation, and are more suitable to be used as tissue engineering scaffold materials.

Key words: electrospinning, nanofiber membranes with large pore sizes, tissue engineering scaffold, polycaprolactone, polyethylene glycol

中图分类号: 

  • TS131.9

图1

改进的静电纺丝装置原理图"

图2

不同PEG和PCL质量比纳米纤维膜扫描电镜照片(×10 000)"

表1

不同PEG和PCL质量比纳米纤维膜的直径统计结果"

PCL与PEG的质量比 平均直径/nm 标准差/nm 置信区间/nm
30:70 187.30 82.88 ±16.24
50:50 223.14 81.45 ±15.96
70:30 306.55 135.39 ±26.54
80:20 336.40 139.66 ±27.37
90:10 364.01 295.17 ±57.85

表2

不同PCL和PEG质量比纳米纤维膜的力学性能"

PCL与PEG的质量比 断裂强度/MPa 断裂伸长率/%
30:70 0.62 25.13
50:50 0.90 57.13
70:30 1.51 83.80
80:20 2.46 87.15
90:10 3.71 92.43

图3

不同PCL/PEG质量分数纳米纤维的形貌(×10 000)"

表3

不同PCL/PEG质量分数纳米纤维的直径统计结果"

溶液质量分数/% 平均直径/nm 标准差/nm 置信区间/nm
15 130.56 52.01 ±10.19(微球)
20 168.63 86.62 ±16.98(微球)
25 335.95 136.41 ±26.74
30 340.23 138.19 ±27.08

表4

不同PCL/PEG质量分数纳米纤维膜的力学性能"

溶液质量分数/% 断裂强度/MPa 断裂伸长率/%
15 0.31 68.91
20 0.81 85.15
25 2.46 87.14
30 2.78 93.40

图4

传统和改进静电纺PCL/PEG纳米纤维膜的扫描电镜照片(×2 000)"

表5

传统和改进静电纺PCL/PEG纳米纤维膜的孔面积统计结果"

纺丝方式 平均孔面积/μm2 标准差/μm2 置信区间/μm2
传统静电纺 9.00 2.94 ±0.41
改进静电纺 15.22 3.59 ±0.50

图5

人脐静脉内皮细胞在纳米纤维膜上的黏附和增殖情况"

图6

培养不同时间时人脐静脉内皮细胞在传统和改进的纳米纤维膜上的扫描电镜照片(×600)"

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