Abstract: To probe into the effective methods to make the natural bamboo fiber finer with enzymatic treatment,the manufactured natural bamboo fibers were treated with laccase,scouring enzyme,and scouring enzyme +laccase(a two step method) respectively according to the design by means of orthogonal experiment.The change rate of fineness,tensile strength and the content of lignin of the treated fibers were tested and compared.The results show that in terms of lignin removal,scouring enzyme is better than laccase,whereas the two step method is the best.After two step treatment,the change rate of fineness of natural bamboo fibers has achieved 51.33% and the content of lignin is decreased from 18.98% to 5.49%.