纺织学报 ›› 2019, Vol. 40 ›› Issue (04): 170-176.doi: 10.13475/j.fzxb.20180300407

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辣根过氧化物酶在纤维材料生物整理中的应用研究进展

周步光, 王平(), 王强, 范雪荣, 袁久刚   

  1. 生态纺织教育部重点实验室(江南大学), 江苏 无锡 214122
  • 收稿日期:2018-03-01 修回日期:2018-12-12 出版日期:2019-04-15 发布日期:2019-04-16
  • 通讯作者: 王平
  • 作者简介:周步光(1994—),男,硕士生。主要研究方向为纺织品生态加工技术。
  • 基金资助:
    国家自然科学基金项目(51373071);国家自然科学基金项目(31771039);青蓝工程资助项目(苏教师[2016]15号);中央高校基本科研业务费专项资金项目(JUSRP51717A);高等学校学科创新引智计划项目(B17021)

Research progress of horseradish peroxidase in bio-finishing of fiber materials

ZHOU Buguang, WANG Ping(), WANG Qiang, FAN Xuerong, YUAN Jiugang   

  1. Key Laboratory of Eco-Textiles (Jiangnan University), Ministry of Education, Wuxi, Jiangsu 214122, China
  • Received:2018-03-01 Revised:2018-12-12 Online:2019-04-15 Published:2019-04-16
  • Contact: WANG Ping

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摘要：

针对化学法整理纤维材料存在能耗高、纤维损伤大的缺陷,提出借助酶法在温和条件下对纤维材料进行生物整理加工。介绍了辣根过氧化物酶/双氧水/β-二酮类引发剂乙酰丙酮(HRP/H2O2/ACAC)三元催化体系的氧化机制,综述了该体系在淀粉、黄麻和丝蛋白生物改性中的应用,包括:通过酶促反应使丙烯酸甲酯与淀粉接枝共聚,提高淀粉浆料对疏水性纤维的成膜性;通过催化黄麻与丙烯酰胺或甲基丙烯酸六氟丁酯接枝,实现黄麻亲水或疏水化整理;通过酶促反应使丝素与丙烯酸接枝共聚,提升丝素材料的仿生矿化效果;通过催化丝胶与甲基丙烯酸甲酯接枝共聚,改善丝胶基生物材料的成型性。指出HRP在纤维整理及生物材料制备中具有潜在的应用前景。

关键词: 辣根过氧化物酶, 三元催化体系, 乙烯基单体, 淀粉, 黄麻, 丝蛋白, 生物材料

Abstract:

Considering the defects that chemical finishing on fiber materials has large energy consumption and potential fiber damages, enzymatic finishing of fiber materials under mild treating conditions were suggested. The oxidation mechanism of the ternary catalyst system of horseradish peroxidase (HRP), hydrogen peroxide (H2O2) and β-diketone initiator acetylacetone (ACAC) were introduced, and its applications in bio-modifications of starch size, jute, silk protein were reviewed as follows. Methyl acrylate was graft copolymerized with starch to improve its film forming property onto the hydrophobic fibers. Acrylamide and hexafluorobutyl methacrylate were applied to modify jute fiber by enzymatic graft-copolymerization, respectively, realizing the hydrophilic or hydrophobic modification of jute fiber. Acrylic acid was used to enzymatically graft copolymerized onto silk fibroin to enhance the biomimetic mineralization effect of fibroin-based biomaterial. Furthermore, HRP-mediated graft copolymerization of methyl methacrylate onto silk sericin was also investigated to improve the formability of sericin-based biomaterials. In conclusion, HRP exhibits potential applications in bio-finishing of textile fibers and preparation of biomaterials.

Key words: horseradish peroxidase, ternary catalyst system, vinyl monomer, starch, jute, silk protein, biomaterial

中图分类号: 

  • TS195.5

图1

HRP/ACAC/H2O2催化机制"

图2

HRP催化淀粉接枝丙烯酸甲酯反应示意图"

图3

HRP催化黄麻纤维中木质素与丙烯酰胺接枝共聚反应示意图"

图4

HRP催化疏水性乙烯基单体和黄麻接枝共聚"

图5

HRP催化丝素蛋白分子间交联"

图6

HRP催化丝素与丙烯酸接枝共聚形成SF-g-PAA示意图"

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