纺织学报 ›› 2019, Vol. 40 ›› Issue (07): 113-118.doi: 10.13475/j.fzxb.20180704906

• 染整与化学品 • 上一篇    下一篇

固载化交联蛋白酶聚集体制备及其对蚕丝织物的砂洗效果

白刚1,2, 刘艳春1,2, 钱红飞1,2   

  1. 1. 绍兴文理学院 纺织服装学院, 浙江 绍兴 312000
    2. 浙江省清洁染整技术研究重点实验室, 浙江 绍兴 312000
  • 收稿日期:2018-07-20 修回日期:2019-04-18 出版日期:2019-07-15 发布日期:2019-07-25
  • 作者简介:白刚(1971-),男,副教授,博士。主要研究方向为纺织品生态染整加工。E-mail: gbg2000@21cn.com。
  • 基金资助:
    浙江省基础公益研究计划项目(LCJ18E030002);浙江省基础公益研究计划项目(LCF18E090006)

Preparation of immobilized cross-linked protease aggregate and sanding effect thereof on silk fabric

BAI Gang1,2, LIU Yanchun1,2, QIAN Hongfei1,2   

  1. 1. College of Textiles and Fashion, Shaoxing University, Shaoxing, Zhejiang 312000, China
    2. Zhejiang Key Laboratory of Clean Dyeing and Finishing Technology, Shaoxing, Zhejiang 312000, China
  • Received:2018-07-20 Revised:2019-04-18 Online:2019-07-15 Published:2019-07-25

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摘要：

针对传统碱剂砂洗中蚕丝强力下降明显及褪色严重等问题,利用固载化交联蛋白酶聚集体(ICLPAs)对蚕丝进行砂洗整理。通过对ICLPAs制备工艺条件进行优化,探讨了ICLPAs的稳定性和重复使用性。通过对KES织物风格、毛效、透气性、透湿性、热阻、湿阻、悬垂性、强力的测试,分析了ICLPAs对蚕丝织物的砂洗效果。结果表明:与游离蛋白酶相比,ICLPAs的热稳定性、pH值稳定性和重复使用性均得到提高;利用ICLPAs对蚕丝织物进行砂洗处理,部分丝素发生水解帚化,织物表面产生微绒,织物的柔软性、抗皱性、吸湿透湿性、透气性提高,断裂强力下降;ICLPAs处理后蚕丝织物的弯曲刚度为0.003 1 cN·cm,悬垂系数为1.97%,折皱回复角为225°,毛效(30 min)为11.3 cm,透气性为372.1 L/(m 2·s),透湿性为2 303 g/(m 2·d)。

关键词: 蚕丝, 砂洗, 固载化, 蛋白酶, 稳定性

Abstract: Aim

ing at the obvious strength decrease and serious fading in silk sanding with conventional alkali agent, the immobilized cross-linked protease aggregates (ICLPAs) were used for sanding of silk fabrics. The preparation conditions of ICLPAs were optimized, and the stability and reusability of ICLPAs were investigated. The properties of ICLPAs-treated fabric were studied by testing KES fabric style, capillary effect, air permeability,moisture penetrability, thermal resistance, wet resistance, drapability and strength. The results show that the thermal stability, pH stability and reusablility of ICLPAs are all improved compared to free protease. The silk fibroin of the ICLPAs-treated fabric is hydrolyzed. The microvelvet is produced on the fabric surface. The softness, wrinkle resistance, moisture absorption, moisture permeability, air permeability increase and the breaking strength decrease. The bending rigidity of the ICLPAs-treated fabric is 0.003 1 cN·cm and drape coefficient is 1.97%. The crease recovery angle is 225°. The capillary effect (30 min) of the treated fabric is 11.3 cm. The air permeability is 372.1 L/(m 2·s) and the moisture penetrability is 2 303 g/(m 2·d).

Key words: silk, sanding, immobilization, protease, stability

中图分类号: 

  • TQ314.1

表1

ICLPAs制备正交试验因素水平表"

水平 A B C D
沉降剂
体积/mL		 载体质量/
mg		 交联剂
质量分数/%		 交联时间/
min
1 2 10 1 20
2 4 20 2 40
3 6 30 3 60
4 8 40 4 80

表2

ICLPAs制备正交试验结果"

序号 A B C D 酶活回收率/%
1 1 1 1 1 52.4
2 1 2 2 2 57.2
3 1 3 3 3 63.2
4 1 4 4 4 54.4
5 2 1 2 3 64.8
6 2 2 1 4 71.2
7 2 3 4 1 59.6
8 2 4 3 2 61.2
9 3 1 3 4 84.4
10 3 2 4 3 87.6
11 3 3 1 2 92.8
12 3 4 2 1 68.4
13 4 1 4 2 81.3
14 4 2 3 1 61.1
15 4 3 2 4 86.8
16 4 4 1 3 78.4
K1 56.8 70.7 73.7 60.4
K2 64.2 69.3 69.3 73.1
K3 83.3 75.6 67.5 73.5
K4 76.9 65.6 70.7 74.2
R 26.5 10.0 6.2 13.8

图1

游离蛋白酶和ICLPAs扫描电镜照片(×1 000)"

图2

载体制备示意图"

图3

固载化交联蛋白酶聚集体的合成路线"

图4

固定化酶的热稳定性"

"

图6

ICLPAs的重复使用性"

图7

ICLPAs处理前后织物扫描照片"

表3

织物弯曲刚度、悬垂系数和折皱回复角"

样品 弯曲刚度/
(cN·cm)		 悬垂系数/
%		 折皱回复角(T+W)/
(°)
未处理 0.024 5 3.27 184
ICLPAs处理 0.003 1 1.97 225

表4

织物的吸湿性、透气性、透湿性、热阻和湿阻"

样品 毛细效应(30 min)/cm 透湿性/(g·m-2·d-1) 湿阻/(m2·Pa·W-1) 热阻/(m2·℃·W-1) 透气性/(L·m-2·s-1)
未处理 9.1 1 711 5.112 0.018 6 339.5
ICLPAs处理 11.3 2 303 4.526 0.019 3 372.1
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