纺织学报 ›› 2019, Vol. 40 ›› Issue (10): 7-12.doi: 10.13475/j.fzxb.20181003306

• 纤维材料 • 上一篇    下一篇

丝素蛋白/壳聚糖微球制备及其抗菌性能

张治斌1,2, 李刚1,2(), 毛森贤3, 厉巽巽3, 陈玉霜3, 毛青山3, 李翼4, 潘志娟1,2, 王晓沁1,2   

  1. 1.苏州大学 纺织与服装工程学院, 江苏 苏州 215123
    2.苏州大学 现代丝绸国家工程实验室, 江苏苏州 215123
    3.浙江真爱美家股份有限公司, 浙江 金华 322000
    4.曼切斯特大学 材料学院, 曼切斯特 M139PL
  • 收稿日期:2018-10-18 修回日期:2019-05-11 出版日期:2019-10-15 发布日期:2019-10-23
  • 通讯作者: 李刚
  • 作者简介:张治斌(1989—),男,硕士生。主要研究方向为抗菌纺织品。
  • 基金资助:
    国家自然科学基金项目(51603140);中国纺织工业联合会指导项目(2018054);中国化纤工业协会-绿宇基金项目(2018914);中国博士后科学基金项目(2017M620223);南通市科技计划项目(GY12017002)

Preparation and antibacterial activity of silk fibroin/chitosan microspheres

ZHANG Zhibin1,2, LI Gang1,2(), MAO Senxian3, LI Xunxun3, CHEN Yushuang3, MAO Qingshan3, LI Yi4, PAN Zhijuan1,2, WANG Xiaoqin1,2   

  1. 1. College of Textile and Clothing Engineering, Soochow University, Suzhou, Jiangsu 215123, China
    2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China
    3. Zhejiang Truelove Vogue Co., Ltd., Jinhua, Zhejiang 322000, China
    4. School of Materials, The University of Manchester, Manchester M139PL, UK
  • Received:2018-10-18 Revised:2019-05-11 Online:2019-10-15 Published:2019-10-23
  • Contact: LI Gang

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摘要：

为开发一种对人体有益且天然无害的抗菌整理剂,采用乳液聚合法制备丝素蛋白/壳聚糖抗菌微球,分析了不同比例下丝素蛋白和壳聚糖制备微球的微观形貌、粒径、二级结构、晶体结构、热稳定性和抗菌性能。结果表明:丝素蛋白质量分数为6%,壳聚糖溶液的质量分数为3%,壳聚糖溶液与丝素蛋白溶液的体积配比为1∶2,所制备的微球形态规整圆滑,粒径均匀分布在0.4~1.4 μm之间;其抗菌效果优异,对大肠杆菌和金黄色葡萄球菌的抑菌率分别为(82±4.2)%和(80±2.6)%;在该比例条件下制备的微球的二级结构由silk I型转变为silk II型,热分解起始温度大于250 ℃,具有良好的热稳定性。

关键词: 丝素蛋白, 壳聚糖, 微球, 抗菌性能, 乳液聚合法

Abstract:

In order to develop a natural and harmless antibacterial finishing agent which is beneficial to human body, silk fibroin/chitosan microspheres were prepared by emulsion polymerization. The microscopic morphology, chemical structure, thermal stability and antibacterial properties of the microspheres with different ratios of silk fibroin and chitosan were examined. The results show that the silk fibroin mass fraction is 6%, the chitosan mass fraction is 3%, and the volume ratio of chitosan to silk fibroin is 1∶2, the microspheres have a spherical shape and smooth surface with particle size between 0.4 to 1.4 μm. In addition, it has a good antibacterial effect against Escherichia coli and Staphylococcus aureus, with the antibacterial ratio of(82 ± 4.2)% and(80 ± 2.6)%, respectively. The secondary structure of the microspheres changes from silk I to silk II, and the initiation temperature of thermal decomposition is higher than 250 ℃, showing good thermal stability.

Key words: silk fibroin, chitosan, microsphere, antibacterial property, emulsion polymerization

中图分类号: 

  • TS141.8

图1

不同CS和SF体积比的微球扫描电镜照片"

图2

不同CS和SF体积比的微球X射线衍射光谱图"

图3

丝素蛋白、壳聚糖及丝素蛋白/壳聚糖微球的傅里叶变换红外光谱图"

图4

丝素蛋白、壳聚糖及丝素蛋白/壳聚糖微球的热稳定性曲线"

图5

SF和CS/SF微球对大肠杆菌的抗菌效果"

图6

SF和CS/SF微球对金黄色葡萄球菌的抗菌效果"

图7

不同CS和SF体积比的微球的抗菌性能"

图8

丝素/壳聚糖成球机制图"

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