二氧化硅包覆银铜纳米颗粒的结构及其抗菌性能

doi:10.13475/j.fzxb.20191105407

摘要/Abstract

摘要：

为研究双金属纳米颗粒间协同抗菌作用及防止金属纳米颗粒团聚,利用气溶胶一步法制备了“火龙果”型高负载量(50%)二氧化硅包覆银铜双金属纳米颗粒抗菌剂Ag-Cu/SiO₂。借助X射线衍射仪、透射电子显微镜、电子能谱仪对Ag-Cu/SiO₂的结构进行研究,并测试该材料对金黄色葡萄球菌和大肠杆菌的最低抑菌浓度(MIC)及细菌的时间与杀菌曲线,研究了2种细菌胞内活性氧的生成情况。结果表明:银铜双金属纳米颗粒均匀分散在球型二氧化硅内部,呈现“火龙果”型结构;与Cu/SiO₂和Ag/SiO₂相比,相同负载量(50%)的Ag-Cu/SiO₂有更好的抗菌效果,对2种细菌的MIC值均为2 μg/mL,在24 h内可以充分抑制细菌生长;Ag-Cu/SiO₂生成活性氧的水平明显高于单金属纳米颗粒,致使细菌死亡,从而说明双金属纳米颗粒具有协同抗菌作用。

关键词: 二氧化硅, 气溶胶法, 抗菌剂, 金属纳米颗粒, 活性氧, 抗菌性能

Abstract:

In order to study the synergistic antibacterial effect between bimetallic nanoparticles and prevent metal nanoparticle agglomeration, a ″dragon fruit″ type of high-load (50%) silica coated silver-copper bimetallic nanoparticle antibacterial agent (Ag-Cu/SiO₂) was prepared using the aerosol one-step method. The structure of Ag-Cu/SiO₂ was characterized by X-ray diffraction, transmission electron microscopy, electron spectroscopy, and the minimum inhibitory concentration (MIC) and time-kill curves of 50%Ag-Cu/SiO₂ against Staphylococcus aureus and Escherichia coli was also studied. The formation of reactive oxygen species (ROS) in bacterial cells were subsequently investigated. The results show that the silver-copper bimetallic nanoparticles uniformly are dispersed in the spherical silica, presenting a "dragon fruit" structure. The Ag-Cu/SiO₂ has better antibacterial properties than Cu/SiO₂ and Ag/SiO₂ with the same loading (50%). The MIC of Ag-Cu/SiO₂against both bacteria was 2 μg/mL and the growth of bacteria was fully inhibited within 24 h. The level of ROS produced by Ag-Cu/SiO₂ is significantly higher than that of single metal nanoparticles and it causes the bacteria to die, indicating that the bimetallic nanoparticles have synergistic antibacterial effect.

Key words: silica, aerosol method, antibacterial agent, metallic nanoparticle, reactive oxygen species, antibacterial property

中图分类号:

O614.12

姜兴茂, 刘奇, 郭琳. 二氧化硅包覆银铜纳米颗粒的结构及其抗菌性能[J]. 纺织学报, 2020, 41(11): 102-108.

JIANG Xingmao, LIU Qi, GUO Lin. Structure and antibacterial properties of silica coated silver-copper nanoparticles[J]. Journal of Textile Research, 2020, 41(11): 102-108.

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样品	S.aureus	E.coli
SiO₂	>256	>256
Cu/SiO₂	>256	>256
Ag/SiO₂	8	4
Ag-Cu/SiO₂	2	2

样品	相对荧光强度
样品	S.aureus	E.coli
SiO₂	1 222	1 915
Cu/SiO₂	1 348	2 730
Ag/SiO₂	2 177	4 404
Ag-Cu/SiO₂	3 902	4 831