纺织学报 ›› 2021, Vol. 42 ›› Issue (01): 35-39.doi: 10.13475/j.fzxb.20200405405

• 纤维材料 • 上一篇    下一篇

羊毛角蛋白在巯基乙酸胆碱中的溶解再生

袁久刚(), 季吉, 薛琪, 姜哲, 范雪荣, 高卫东   

  1. 生态纺织教育部重点实验室(江南大学), 江苏 无锡 214122
  • 收稿日期:2020-04-10 修回日期:2020-09-28 出版日期:2021-01-15 发布日期:2021-01-21
  • 作者简介:袁久刚(1982—),男,副教授,博士。主要研究方向为天然蛋白质材料提取及改性。E-mail: jiugangyuan@jiangnan.edu.cn。
  • 基金资助:
    国家自然科学基金项目(31771039);国家重点研发计划专项资助项目(2017YFB0309200);中央高校基本科研业务费专项资金项目(JUSRP51717A);江苏省高等学校大学生创新创业训练计划项目(201910295064Z)

Dissolution and regeneration of wool keratin in choline thioglycolate

YUAN Jiugang(), JI Ji, XUE Qi, JIANG Zhe, FAN Xuerong, GAO Weidong   

  1. Key Laboratory of Eco-Textiles (Jiangnan University), Ministry of Education, Wuxi, Jiangsu 214122, China
  • Received:2020-04-10 Revised:2020-09-28 Online:2021-01-15 Published:2021-01-21

RichHTML

15

PDF (PC)

79

摘要：

为提高废弃羊毛纤维的综合利用率,采用一种新型的还原性类离子液体巯基乙酸胆碱对羊毛进行溶解和再生,观察了羊毛纤维在其中的溶解过程。采用红外光谱仪、X射线衍射分析仪、差示扫描量热仪以及凝胶电泳仪对不同温度下提取的再生角蛋白性能进行了研究。结果表明:巯基乙酸胆碱能够有效打开羊毛角蛋白大分子之间的二硫键,从而实现对羊毛皮质层及鳞片层的高效溶解,120 ℃条件下,最高溶解质量分数可以达到16%;经巯基乙酸胆碱溶解再生制得的角蛋白,其蛋白主体结构得到保留,但是与原羊毛相比,再生角蛋白中的α-螺旋含量降低,分子质量降低,并且随着溶解温度提高,角蛋白大分子也发生了一定程度降解。

关键词: 羊毛, 角蛋白, 巯基乙酸胆碱, 离子液体, 二硫键

Abstract:

In order to improve the solubility and utilization of waste wool fiber, a new type of reducing ionic liquid-choline thioglycolate, was employed for wool dissolution in the reported research. The dissolution process of a single wool fibre in choline thioglycolate was carefully observed using polarizing optical microscope. The wool keratin extracted at different temperatures was characterized through fourier transform infrared spectrometer,X-ray diffraction,differential scanning calorimetry and SDS-PAGE in detail. The results show that choline thioglycolate is able to effectively destroy the disulfide bonds in wool keratin, thus achieving efficient dissolution of the wool cortex and scales. At 120 ℃, the dissolution of wool in choline thioglycolate reached up to 16%, and the regenerated wool keratin retained the main body structure. However, compared with raw wool fibers, the α-helix content in the regenerated keratin and the molecular weight decreased, and the keratin macromolecules degraded seriously under higher temperature.

Key words: wool, keratin, choline thioglycolate, ionic liquid, disulfide bond

中图分类号: 

  • TS195.5

图1

羊毛在巯基乙酸胆碱中的溶解速率曲线"

图2

羊毛纤维在巯基乙酸胆碱中溶解过程"

图3

皮质层在巯基乙酸胆碱中溶解过程"

图4

鳞片层在巯基乙酸胆碱中溶解过程"

图5

原羊毛及再生角蛋白红外光谱图"

图6

羊毛及再生角蛋白的X射线衍射图谱"

表1

羊毛及再生角蛋白结晶指数"

样品 溶解温度/℃ I9/a.u. I14/a.u. CI
原毛 592 278 0.53
再生角蛋白 110 340 224 0.34
再生角蛋白 120 380 266 0.30
再生角蛋白 130 388 291 0.25

图7

再生角蛋白差示扫描量热图"

图8

羊毛和再生角蛋白的SDS-PAGE图 a—标准蛋白;b—再生角蛋白(110 ℃); c—再生角蛋白(120 ℃);d—再生角蛋白(130 ℃);e—原羊毛。"

[1] ANNALISA A, CLAUDIO T, MARINA Z. Characterization of keratin biomass from butchery and wool industry wastes[J]. Journal of Molecular Structure, 2009,938(1):35-40.
[2] LI R, WANG D. Preparation of regenerated wool keratin films from wool keratin-ionic liquid solu-tions[J]. Journal of Applied Polymer Science, 2013,127(4):2648-2653.
[3] 翟培羽. 高角蛋白含量的角蛋白/PEO纳米纤维的制备与表征[D]. 天津:天津工业大学, 2017: 9-10.
ZHAI Peiyu. Preparation and characterization of high keratin content keratin/PEO nanofibers[D]. Tianjin: Tiangong University, 2017: 9-10.
[4] SEDDON K R. Ionic liquids: fact and fiction[J]. Chemistry Today, 2006,24(2):16-23.
[5] HOLBREY J D, SEDDON K R. Ionic liquids[J]. Clean Products and Processes, 1999,1(4):223-236.
[6] XIE H B, ZHANG S B, LI S H. Ionic liquids as novel solvents for the dissolution and blending of wool keratin fibers[J]. Green Chemistry, 2005,7(8):606-608.
[7] SUN P, LIU Z T, LIU Z W. Particles from bird feather: a novel application of an ionic liquid and waste resource[J].Journal of Hazardous Materials, 2009(170):786-790.
[8] 袁久刚. 离子液体-蛋白酶处理对羊毛表面性能的影响[D]. 无锡:江南大学, 2010: 95.
YUAN Jiugang. Effects of ionic liquid-protease treatment on the surface properties of wool[D]. Wuxi: Jiangnan University, 2010: 95.
[9] 孙艳丽, 姚金波, 李博, 等. 羊毛角蛋白溶液的离子液体法制备[J]. 纺织学报, 2015,36(5):59-62.
SUN Yanli, YAO Jinbo, LI Bo, et al. Preparation of wool keratin solution by ionic liquid method[J]. Journal of Textile Research, 2015,36(5):59-62.
[10] STEWART K, SPEDDING P L, OTTERBURN M S, et al. Isolating the cuticle layer of wool: a comparison of methods[J]. Journal of the Society of Dyers and Colourists, 1997,113(1):32-34.
[11] 张振雷. 离子液体溶解角蛋白过程及机理研究[D]. 北京:中国科学院大学, 2016: 25-26.
ZHANG Zhenlei. Research on the process and mechanism of dissolving keratin by ionic liquis[D]. Beijing: University of Chinese Academy of Science, 2016: 25-26.
[12] MICHAEL J, HENRY H M. The use and misuse of ftir spectroscopy in the determination of protein struc-ture[J]. Critical Reviews in Biochemistry and Molecular Biology, 1995,30(2):95-120.
doi: 10.3109/10409239509085140 pmid: 7656562
[13] SEGAL L, CREELY J J, MARTIN A E, et al. An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffreactometer[J]. Textile Research Journal, 1959,29(10):786-794
[14] KHOSA M A, ULLAH A. In-situ modification, regeneration, and application of keratin biopolymer for arsenic removal[J]. Journal of Hazardous Materials, 2014,278:360-371.
pmid: 24996154
[15] ZHANG J, LI Y, LI J S, et al. Isolation and characterization of bio-functional keratin particles extracted from wool wastes[J]. Powder Technology, 2013,246:356-362.
[16] CAO J N, BHOYRO A Y. Structural characterization of wool by thermal mechanical analysis of yarns[J]. Textile Research Journal, 2001,71(1):63-66.
[17] VASCONCELOS A, FREDDI G, CAVACOPAULO A, et al. Biodegradable materials based on silk fibroin and keratin[J]. Biomacromolecules, 2008,9(4):1299-1305.
pmid: 18355027
[18] SPEI M, HOLZEM R. Thermoanalytical determination of the relative helix content of keratins[J]. Colloid & Polymer Science, 1989,267(6):549-551.
[1] 于佳, 辛斌杰, 卓婷婷, 周曦. 高导电性铜/聚吡咯涂层羊毛织物的制备与表征[J]. 纺织学报, 2021, 42(01): 112-117.
[2] 欧阳鹏飞, 张玉芳, 贾春紫, 张嘉煜. 用竹浆粕/ 离子液体复配体系纺制的再生纤维及其性能 [J]. 纺织学报, 2020, 41(01): 21-25.
[3] 崔桂新, 董永春, 王鹏. 羊毛/铁配合物非均相芬顿反应光催化剂的制备及其应用性能[J]. 纺织学报, 2019, 40(12): 68-73.
[4] 涂莉, 孟家光, 李欣, 李娟子. 废旧毛/丝/棉混纺面料的组分分析及其剥色工艺[J]. 纺织学报, 2019, 40(11): 75-80.
[5] 韦玲俐, 邹沁杉, 王璐, 罗菁, 夏鑫. 无氟自清洁功能型羊毛/羊绒混纺织物的研发[J]. 纺织学报, 2019, 40(09): 102-107.
[6] 张景清, 粟晖, 梁晓芸, 陈维骥, 姚志湘. 基于斜投影的纯羊毛毛线中酸性红26的快速检测[J]. 纺织学报, 2019, 40(08): 85-88.
[7] 包红, 徐水, 张小宁, 成国涛, 朱勇. 家蚕丝素蛋白阳离子化及其对羊毛性状的影响[J]. 纺织学报, 2019, 40(07): 24-30.
[8] 梅静霞, 张楠, 王强, 袁久刚, 范雪荣. 基于修饰蛋白酶的羊毛织物防毡缩整理[J]. 纺织学报, 2019, 40(06): 73-78.
[9] 安芳芳, 房宽峻, 刘秀明, 蔡玉青, 韩双, 杨海贞. 羊毛织物的蛋白酶改性对墨滴铺展及颜色性能的影响[J]. 纺织学报, 2019, 40(06): 58-63.
[10] 刘淑萍, 李亮, 刘让同, 崔世忠, 王艳婷. 羧甲基纤维素钠改性角蛋白膜的结构与性能[J]. 纺织学报, 2019, 40(06): 14-19.
[11] 邢文宇, 邓娜, 辛斌杰, 于晨. 基于多特征融合图像分析技术的羊毛与羊绒鉴别[J]. 纺织学报, 2019, 40(03): 146-152.
[12] 陈静静, 王必其, 王雪琴. 针刺复合羊毛面料的设计及其性能[J]. 纺织学报, 2019, 40(03): 49-53.
[13] 李博, 姚金波, 牛家嵘, 王乐, 冯懋, 孙艳丽. 采用还原剂-甲酸法溶解制备羊毛角蛋白质溶液[J]. 纺织学报, 2019, 40(03): 1-7.
[14] 俞俭 逄增媛 魏取福. 聚苯胺/壳聚糖/羊毛复合织物导电性能及苯胺吸附分子模拟[J]. 纺织学报, 2018, 39(12): 95-100.
[15] 张淑梅 姬春林 殷秀梅 潘峰 毛鑫磊. 羊毛生物酶联合防毡缩整理[J]. 纺织学报, 2018, 39(11): 85-90.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备14006648号　 京公网安备11010502044800号
版权所有 © 2021 《纺织学报》编辑部
地址： 北京市朝阳区延静里中街3号主楼6层《纺织学报》杂志社　邮政编码：100025　
电话：010-65017711,65917740　传真：010-65016539　Email:fangzhixuebao@vip.126.com
本系统由北京玛格泰克科技发展有限公司设计开发