纺织学报 ›› 2022, Vol. 43 ›› Issue (06): 49-56.doi: 10.13475/j.fzxb.20210503908

• 纤维材料 • 上一篇    下一篇

纳米纤维基单向导湿抗菌敷料的制备及其性能

欧康康1,2,3, 祁琳雅1, 侯怡君1, 范天华1, 齐琨1(), 王宝秀2, 王华平2,3   

  1. 1.中原工学院 纺织服装产业研究院, 河南 郑州 451191
    2.东华大学 纤维材料改性国家重点实验室, 上海 201620
    3.国家先进功能纤维创新中心, 江苏 苏州 215228
  • 收稿日期:2021-05-17 修回日期:2021-12-22 出版日期:2022-06-15 发布日期:2022-07-15
  • 通讯作者: 齐琨
  • 作者简介:欧康康(1990—),男,副教授,博士。主要研究方向为生物医用纳米纤维。
  • 基金资助:
    国家自然科学基金项目(52003048);河南省重点研发与推广专项(212102210041);河南省大学生创新创业训练计划项目(S202110465084);中国纺织工业联合会科技指导项目(2019028);中原工学院青年自然科学基金项目(K2022QN013);中原工学院青年骨干教师项目(2021XQG02)

Preparation and properties of nanofiber-based unidirectional water-transport antibacterial wound dressings

OU Kangkang1,2,3, QI Linya1, HOU Yijun1, FAN Tianhua1, QI Kun1(), WANG Baoxiu2, WANG Huaping2,3   

  1. 1. Research Institute of Textile and Clothing Industries, Zhongyuan University of Technology, Zhengzhou, Henan 451191, China
    2. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China
    3. National Advanced Functional Fiber Innovation Center, Suzhou, Jiangsu 215228, China
  • Received:2021-05-17 Revised:2021-12-22 Published:2022-06-15 Online:2022-07-15
  • Contact: QI Kun

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摘要：

针对现有湿性敷料易滋生细菌并在创伤处形成积液的问题,以力学性能优良的聚氨酯(PU)、亲水性聚丙烯腈和超吸水性的聚丙烯酸钠为基材,以聚六亚甲基胍盐酸盐(PHGC)为抗菌剂,采用静电纺丝法制备出由纤维尺寸不同的亲水外层和疏水内层组成的纳米纤维基双层敷料,探究疏水层厚度对双层敷料单向导湿效果的影响,并分析敷料结构与性能之间的关系。结果表明:当PU内层膜纺丝时间为1 h时,所制备的双层敷料可在3.9 s内由内向外单向传输液体,此时敷料的吸水率高达1 230%,透气率约为 6.7 mm/s,透湿率约为1 350 g/(m2·d),断裂强度为 6.5 MPa,断裂伸长率为45%;PU内层膜中添加0.06%的PHGC可使敷料对大肠杆菌和金黄色葡萄球菌的抑菌率达到95%以上,无细胞毒性且具备较低的细胞黏附性能。

关键词: 创伤敷料, 静电纺丝, 单向导湿, 抗菌性能, 纳米纤维

Abstract:

In order to deal with bacteria breeding and fluid formation on wound site, polyurethane (PU) with excellent mechanical properties, polyacrylonitrile with high hydrophilicity and sodium polyacrylate with superabsorbent water were used as the substrate, and polyhexamethyl guanidine hydrochloride (PHGC) was used as the antibacterial agent to create antibacterial wound dressings. The nanofiber-based bilayer dressing material composed of hydrophilic outer layer and hydrophobic inner layer with different fiber sizes was prepared by electrospinning. The influence of hydrophobic layer thickness on the unidirectional water-transport effect of bilayer dressing was investigated, and the relationship between the structure and properties of the dressing were analyzed. The results show that the prepared bilayer dressing material can transfer water unidirectionally from inside to outside in 3.9 s, and the water absorption of the dressing is as high as 1 230%. The air permeability is 6.7 mm/s, the moisture permeability is 1 350 g/(m2·d), the breaking strength is 6.5 MPa and the elongation at break is 45%. The addition of 0.06% PHGC to PU inner layer makes the antibacterial rate of dressing against Escherichia coli and Staphylococcus aureus reach more than 95%. The prepared bilayer dressings show no cytotoxicity and low cell adhesion.

Key words: wound dressing, electrospinning, unidirectional water-transport, antibacterial property, nanofiber

中图分类号: 

  • TS174.1

图1

双层纳米纤维敷料的形貌、直径分布图及其水接触角"

图2

内、外层膜和不同双层敷料正面的动态接触角"

图3

墨滴在样品中的扩散过程"

图4

双层敷料正反向液体传输过程"

表1

双层敷料的物理性能"

样品 透气率/
(mm·s-1)		 透湿率/
(g·m-2·d-1)		 平衡含
水量/%		 吸水
率/%		 断裂
强度/
MPa		 断裂
伸长
率/%
内层 6.2±0.2 1 350±23 78±1.0 377±23 6.0±0.5 40±5
外层 7.5±0.1 1 970±25 93±0.6 970±27 8.0±0.5 80±5
双层
敷料		 6.7±0.5 1 350±20 92±2.0 1 230±50 6.5±0.5 45±5

图5

PHGC和双层敷料的热重曲线图"

图6

PHGC、PU-PHGC和PU纳米纤维膜的红外图谱"

图7

双层敷料的抗菌性能"

图8

内外层膜及敷料浸提液对GES-1细胞活力的影响"

图9

双层敷料培养48 h后的细胞黏附性能"

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