纺织学报 ›› 2023, Vol. 44 ›› Issue (05): 213-219.doi: 10.13475/j.fzxb.20211101502

• 综合述评 • 上一篇    下一篇

含锌和含铜医用敷料的研究进展

秦益民1,2()   

  1. 1.嘉兴学院 材料与纺织工程学院, 浙江 嘉兴 314001
    2.海藻活性物质国家重点实验室, 山东 青岛 266400
  • 收稿日期:2021-11-03 修回日期:2022-06-30 出版日期:2023-05-15 发布日期:2023-06-09
  • 作者简介:秦益民(1965—),男,教授,博士。研究方向为功能性医用敷料的研究与开发。E-mail:yiminqin1965@126.com。
  • 基金资助:
    国家重点研发计划项目(2018YFC1105600)

Research progress in zinc and copper containing wound dressings

QIN Yimin1,2()   

  1. 1. College of Material and Textile Engineering, Jiaxing University, Jiaxing, Zhejiang 314001, China
    2. State Key Laboratory of Bioactive Seaweed Substances, Qingdao, Shandong 266400, China
  • Received:2021-11-03 Revised:2022-06-30 Published:2023-05-15 Online:2023-06-09

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摘要：

为开发功能性含锌和含铜纤维及医用敷料在伤口护理中的应用,介绍了锌离子和铜离子的生物活性及其在伤口愈合过程中的作用,总结了国内外市场上各种含锌和含铜医用敷料及其制备方法,重点介绍了含锌和含铜的海藻酸盐纤维、壳聚糖纤维,以及通过静电纺丝、高分子材料复合加工、纳米技术等先进工艺制备的各种含锌和含铜医用敷料及其释放锌离子和铜离子的性能。指出锌离子和铜离子具有优良的抗菌及促进伤口愈合的功效,含锌和含铜医用敷料通过释放锌离子和铜离子,对创面常见的各种细菌有很强的抑菌作用,在烧伤、下肢溃疡、糖尿病足溃疡、褥疮等伤口护理中有重要的应用价值。

关键词: 锌离子, 铜离子, 功能纤维, 医用敷料, 医用纺织材料, 创面护理

Abstract:

Significance In order to develop the applications of functional zinc and copper containing fibers and wound dressings in the management of chronic wounds, this paper introduced the bioactivities of zinc and copper ions and their roles in the wound healing process, and summarized the types and production methods of zinc and copper containing wound dressings in the domestic and international market, in particular zinc and copper containing alginate fibers, chitosan fibers, and the various types of zinc and copper containing wound dressings and their ion releasing properties obtained by electrospinning, polymeric composites, nano technologies and other advanced processing methods. Both zinc and copper ions possess antimicrobial efficacy and are known to be able to promote wound healing. The incorporation of these two metal ions by the techniques reviewed in this paper can help manufacturers and medical practitioners in the wound management industry to fully utilize the novel health benefitting properties of zinc and copper ions.

Progress Both zinc and copper ions have been used in the wound management industry for a long time by many forms of applications. Recent progresses are mainly focused in the sustainable release of zinc and copper ions by combining these two ions with alginate, chitosan and other novel substrate materials, and also by the use of nanoparticles of zinc oxide and copper oxide. The incorporation of zinc ions into alginate fibers and the subsequent release of zinc ions when in contact with aqueous solutions containing different levels of protein were summarized. The composition and properties of many types of zinc containing wound dressings reported in the documents were summarized. Regarding copper containing wound dressings, important progress has been made by incorporating copper oxide into fibers by blending and the subsequent extrusion to form copper containing fibers. In addition, copper ions can be absorbed into various types of alginate fibers that can be released when these fibers are in contact with aqueous solutions similar to wound exudate. Many other methods have been used in the documents to load and release copper ions from the base materials of wound dressings. The combination of zinc and copper ions with wound dressings have demonstrated important clinical benefits by accelerating the healing of burns and chronic wounds. Zinc and copper containing fibers and wound dressings possess antimicrobial properties similar to silver containing fibers and wound dressings.

Conclusion and Prospect Zinc and copper ions are minor metal ions present in the human body and are closely involved in the function of many enzymes during the wound healing process. The loss of zinc ions are well known for the delayed healing of burn wounds. Copper ions are also shown to be able to promote the healing of chronic wounds such as leg ulcers and pressure sores. The overall results of this review showed that zinc and copper ions have excellent antimicrobial properties and can promote wound healing. Experimental results have confirmed that zinc and copper containing wound dressings have strong antibactenal effect against the various types of bacteria commonly present in wounds, and these dressings are highly useful in the management of leg ulcer, pressure sore, diabetic foot ulcer, burn and other types of wounds. Looking into the future, much research and development work is still needed to clarify the wound healing mechanism of these two metal ions. In addition, clinical research and formal clinical trials are also required to validate the clinical efficacy of zinc and copper containing wound dressings before these products can be commercialized. Regulatory approval procedures are also needed through the collaborative efforts of researchers, manufacturers, regulatory bodies and medical practioners around the world.

Key words: zinc ion, copper ion, functional fiber, wound dressing, medical textile material, wound management

中图分类号: 

  • TS131.9

表1

ZnSO4·7H2O用量对海藻酸纤维中锌离子含量的影响"

ZnSO4·7H2O
用量/g		 纤维干态
质量/g		 纤维中锌离子
含量/(mg·g-1)
0.5 0.595 53.1
1.0 0.873 70.3
3.0 0.521 109.0
10.0 0.839 128.5

图1

在不同质量分数的蛋白质水溶液中海藻酸锌纤维释放锌离子的性能"

表2

含锌壳聚糖纤维的制备条件"

样品编号 纤维干态
质量/g		 ZnCl2
质量/g		 纤维质量
增加率/%
1 6.922 0.692 4.26
2 7.647 1.912 9.95
3 9.164 4.582 14.17
4 10.853 21.706 18.67

表3

几种含锌医用敷料的组成和性能"

基础材料 锌化合物 主要性能
含海藻超细粉体的纤维素 锌离子 与伤口渗出液接触后,通过离子交换释放出锌离子[20]
海藻酸气凝胶 锌离子 海藻酸与锌结合形成的气凝胶释放的锌,在RAW 264.7巨噬细胞中有高利用率和抗炎活性[21]
壳聚糖和聚乙烯醇 肝素化纳米氧化锌颗粒 有效加速伤口闭合和再上皮化,显著促进急性创面愈合[22]
芦荟-海藻酸薄膜 锌离子 促进皮肤切口愈合[23]
聚氧化乙烯(PEO)纳米纤维 纳米氧化锌颗粒 比表面积高、亲水性好,适用于作为组织工程支架改善创面愈合[24]
水凝胶与丝素纤维的复合物 氧化锌 使营养物质、生长因子、代谢物和气体的交换更容易,有利于受损组织的成功再生[25]
卡拉胶 纳米氧化锌/L-谷氨酸 复合水凝胶可用于喷洒伤口,消除创面感染、加速伤口愈合[26]
静电纺海藻酸盐基垫 纳米氧化锌粒子 纳米氧化锌粒子具有很强的抗菌性能[27]
海藻酸钙水凝胶+细菌纤维素 锌离子 良好的抑菌性能[28]
50%氯化锌溶液 氯化锌对黑色素瘤切除伤口上组织的杀伤使伤口变得更深更宽[29]

表4

几种含铜医用敷料的组成和性能"

基础材料 铜化合物 主要性能
含海藻超细粉体的纤维素纤维 铜离子 与伤口渗出液接触后,通过离子交换释放出铜离子[20]
醋酸纤维素 纳米氧化铜 细胞活力和抗菌性能显著提高[36]
常规伤口愈合敷料 氧化铜微粒 在伤口上应用氧化铜浸渍的敷料可显著促进伤口愈合[37]
静电纺纳米纤维素纤维 纳米氧化铜 对革兰氏阴性和革兰氏阳性菌均有显著的抗菌性能[38]
细菌纳米纤维素+海藻酸盐 铜离子 具有pH值响应性抗菌活性,加快创面愈合[39]
聚己内酯 纳米氧化铜 抑制耐甲氧西林金黄色葡萄球菌(MRSA)生长[40]
常规伤口敷料 铜离子 在不使用抗生素的情况下减少医院获得性感染及其相关的抗生素耐药性风险[41]
静电纺聚氨酯 硫酸铜 具有良好的理化性能和血液相容性,促进创面愈合[42]

图2

含大肠杆菌的培养液在与含不同金属离子的壳聚糖纤维接触后的浑浊度"

[1] BERGER M M, CAVADINI C, BART A, et al. Cutaneous copper and zinc losses in burns[J]. Burns, 1992, 18(5): 373-380.
pmid: 1445626
[2] AGREN M S. Studies on zinc in wound healing[J]. Acta Dermato-Venereologica Supplementum, 1990, 154: 1-36.
[3] PICKART L. The human tri-peptide GHK and tissue remodeling[J]. J Biomater Sci Polym Ed, 2008, 19(8): 969-988.
doi: 10.1163/156856208784909435
[4] SCHWARTZ J R, MARSH R G, DRAELOS Z D. Zinc and skin health: overview of physiology and pharmacology[J]. Dermatol Surg, 2005, 31(7): 837-847.
doi: 10.1111/(ISSN)1524-4725
[5] 秦益民. Cupron铜基抗菌纤维的性能和应用[J]. 纺织学报, 2009, 30(12):134-136.
QIN Yimin. Properties and applications of Cupron copper containing antimicrobial fibers[J]. Journal of Textile Research, 2009, 30(12):134-136.
[6] 秦益民, 陈洁. 海藻酸纤维吸附及释放锌离子的性能[J]. 纺织学报, 2011, 32(1):16-19.
QIN Yimin, CHEN Jie. Absorption and release of zinc ion by alginate fibers[J]. Journal of Textile Research, 2011, 32(1):16-19.
[7] TENAUD I, SAINTE-MARIE I, JUMBOU O, et al. In vitro modulation of keratinocyte wound healing integrins by zinc, copper and manganese[J]. British Journal of Dermatology, 1999, 140: 26-34.
pmid: 10215764
[8] WILLIAMS K J, METZLER R, BROWN R A, et al. The effects of topically applied zinc on the healing of open wounds[J]. Journal of Surgical Research, 1979, 27: 62-67.
doi: 10.1016/0022-4804(79)90111-2
[9] LANSDOWN A B, MIRASTSCHIJSKI U, STUBBS N, et al. Zinc in wound healing: theoretical, experimental, and clinical aspects[J]. Wound Repair Regen, 2007, 15(1): 2-16.
doi: 10.1111/j.1524-475X.2006.00179.x pmid: 17244314
[10] 李利根, 郭振荣, 赵霖, 等. 口饲和创面补锌对烫伤大鼠血清组织中锌的影响[J]. 西南国防医药, 2006, 16(2):147-149.
LI Ligen, GUO Zhenrong, ZHAO Lin, et al. Effects of oral feeding and wound zinc supplementation on serum zinc in scald rats[J]. Medical Journal of National Defending Forces in Southwest China, 2006, 16(2):147-149.
[11] AGREN M S, STROMBERG H E. Topical treatment of pressure ulcers: a randomized comparative trial of varidase and zinc oxide[J]. Scand J Plast Reconstr Surg, 1985, 19: 97-100.
[12] DAVIES J W, FELL G S. Tissue catabolism in patients with burns[J]. Clin Clim Acta, 1974, 51(1): 83-92.
doi: 10.1016/0009-8981(74)90064-3
[13] 陈国贤, 韩春茂, 王彬. 严重烧伤病人微量元素的动态变化[J]. 肠外与肠内营养, 1998, 5(3):146-148.
CHEN Guoxian, HAN Chunmao, WANG Bin. Dynamic changes in the trace elements of heavily burnt pati-ents[J]. Parenteral & Enteral Nutrition, 1998, 5(3):146-148.
[14] 郭振荣, 李利根, 赵霖, 等. 烧伤后锌代谢特点及其对铜、铁、钙的影响[J]. 中华烧伤杂志, 2000, 16(5): 286-288.
GUO Zhenrong, LI Ligen, ZHAO Lin, et al. Zinc metabolism after burn and its effect on copper, iron and calcium[J]. Chinese Journal of Burns, 2000, 16(5): 286-288.
[15] KOUREMENOU-DONA E, DONA A, PAPOUTSIS J. Copper and zinc concentrations in serum of healthy Greek adults[J]. The Science of the Total Environment, 2006, 359(1-3):76-81.
doi: 10.1016/j.scitotenv.2005.04.009
[16] DOLLWET H H A, SORENSON J R J. Historic uses of copper compounds in medicine[J]. Trace Elements in Medicine, 1985, 2: 80-87.
[17] SHIPPEE R L, MASON A D, BURLESON D G. The effect of burn injury and zinc nutrition on fecal endogenous zinc, tissue zinc distribution, and T-lymphocyte subset distribution using a murine model[J]. Proc Soc Exp Biol Med, 1988, 189(1): 31-38.
doi: 10.3181/00379727-189-42776
[18] PARBOTEEAH S, BROWN A. Managing chronic venous leg ulcers with zinc oxide paste bandages[J]. British Journal of Nursing, 2008, 17(6): 30-36.
[19] QIN Y, ZHU C, CHEN J, et al. Absorption and release of zinc and copper ions by chitosan fibers[J]. Journal of Applied Polymer Science, 2007, 105(2): 527-532.
doi: 10.1002/(ISSN)1097-4628
[20] 秦益民, 陈洁. 海丝纤维吸附铜和锌离子的性能[J]. 纺织学报, 2011, 32(9): 20-23.
QIN Yimin, CHEN Jie. Absorption of copper and zinc ions by seacell fibers[J]. Journal of Textile Research, 2011, 32(9): 20-23.
[21] KEIL C, HUBNER C, RICHTER C, et al. Ca-Zn-Ag alginate aerogels for wound healing applications: swelling behavior in simulated human body fluids and effect on macrophages[J]. Polymers (Basel), 2020, 12(11): 2741-2745.
doi: 10.3390/polym12112741
[22] KHORASANI M T, JOORABLOO A, ADELI H, et al. Enhanced antimicrobial and full-thickness wound healing efficiency of hydrogels loaded with heparinized ZnO nanoparticles: in vitro and in vivo evaluation[J]. Int J Biol Macromol, 2021, 166: 200-212.
doi: 10.1016/j.ijbiomac.2020.10.142 pmid: 33190822
[23] KOGA A Y, FELIX J C, SILVESTRE R G M, et al. Evaluation of wound healing effect of alginate film containing Aloe vera gel and cross-linked with zinc chloride[J]. Acta Cir Bras, 2020. DOI:10.1590/s0102-865020200050000007.
doi: 10.1590/s0102-865020200050000007
[24] NOSRATI H, KHODAEI M, BANITALEBI-DEHKORDI M, et al. Preparation and characterization of poly(ethylene oxide)/zinc oxide nanofibrous scaffold for chronic wound healing applications[J]. Polim Med, 2020, 50(1): 41-51.
doi: 10.17219/pim/128378 pmid: 33150750
[25] MAJUMDER S, DAHIYA U R, YADAV S, et al. Zinc oxide nanoparticles functionalized on hydrogel grafted silk fibroin fabrics as efficient composite dressing[J]. Biomolecules, 2020, 10(5):710-714.
doi: 10.3390/biom10050710
[26] TAVAKOLI S, MOKHTARI H, KHARAZIHA M, et al. A multifunctional nanocomposite spray dressing of Kappa-carrageenan-polydopamine modified ZnO/L-glutamic acid for diabetic wounds[J]. Materials Science and Engineering: C, 2020. DOI: 10.1016/j.msec.2020.110837.
doi: 10.1016/j.msec.2020.110837
[27] DODERO A, SCARFI S, POZZOLINI M, et al. Alginate-based electrospun membranes containing ZnO nanoparticles as potential wound healing patches: biological, mechanical, and physicochemical characterization[J]. ACS Appl Mater Interfaces, 2020, 12(3):3371-3381.
doi: 10.1021/acsami.9b17597
[28] ZHANG M, CHEN S, ZHONG L, et al. Zn2+-loaded TOBC nanofiber-reinforced biomimetic calcium alginate hydrogel for antibacterial wound dressing[J]. Int J Biol Macromol, 2020, 143:235-242.
doi: 10.1016/j.ijbiomac.2019.12.046
[29] BROOKS N A. Treatment of melanoma excision wound with 50% zinc chloride solution astringent-mohs melanoma surgery without the paste[J]. J Clin Aesthet Dermatol, 2020, 13(3):15-16.
[30] BORKOW G, GABBAY J, ZATCOFF R C. Could chronic wounds not heal due to too low local copper levels[J]. Med Hypotheses, 2008, 70(3): 610-613.
doi: 10.1016/j.mehy.2007.06.006
[31] QIN Y, ZHU C, CHEN J, et al. Chitosan fibers with enhanced antimicrobial properties[J]. Chemical Fibers International, 2009(3): 154-156.
[32] GABBAY J, MISHAL J, MAGEN E, et al. Copper oxide impregnated textiles with potent biocidal activ-ities[J]. Journal of Industrial Textiles, 2006, 35: 323-335.
doi: 10.1177/1528083706060785
[33] BORKOW G, GABBAY J. Putting copper into action: copper impregnated products with potent biocidal activities[J]. Faseb Journal, 2004, 18: 1728-1730.
doi: 10.1096/fsb2.v18.14
[34] BORKOW G, LARA H H, COVINGTON C Y, et al. Deactivation of human immunodeficiency virus type 1 in medium by copper oxide-containing filters[J]. Antimicrob Agents Chemother, 2008, 52(2): 518-525.
doi: 10.1128/AAC.00899-07 pmid: 18070974
[35] 莫岚, 陈洁, 宋静, 等. 海藻酸纤维对铜离子的吸附性能[J]. 合成纤维, 2009, 38(2):34-36.
MO Lan, CHEN Jie, SONG Jing, et al. Absorption of copper ions by alginate fibers[J]. Synthetic Fibers, 2009, 38(2):34-36.
[36] AL-SAEEDI S, AL-KADHI N S, AL-SENANI G M, et al. Antibacterial potency, cell viability and morphological implications of copper oxide nanoparticles encapsulated into cellulose acetate nanofibrous scaffolds[J]. Int J Biol Macromol, 2021, 182:464-471.
doi: 10.1016/j.ijbiomac.2021.04.013
[37] MELAMED E, KIAMBI P, OKOTH D, et al. Healing of chronic wounds by copper oxide-impregnated wound dressings-case series[J]. Medicina (Kaunas), 2021, 57(3): 296-300.
[38] HAIDER M K, ULLAH A, SARWAR M N, et al. Lignin-mediated in-situ synthesis of CuO nanoparticles on cellulose nanofibers: a potential wound dressing material[J]. Int J Biol Macromol, 2021, 173:315-326.
doi: 10.1016/j.ijbiomac.2021.01.050 pmid: 33450343
[39] SHAHRIARI-KHALAJI M, HONG S, HU G, et al. Bacterial nanocellulose-enhanced alginate double-network hydrogels cross-linked with six metal cations for antibacterial wound dressing[J]. Polymers (Basel), 2020, 12(11): 2683-2688.
doi: 10.3390/polym12112683
[40] BALCUCHO J, NARVAEZ D M, CASTRO-MAYORGA J L. Antimicrobial and biocompatible polycaprolactone and copper oxide nanoparticle wound dressings against methicillin-resistant Staphylococcus aureus[J]. Nanomaterials (Basel), 2020, 10(9):1692-1697.
doi: 10.3390/nano10091692
[41] ARENDSEN L P, THAKAR R, BASSETT P, et al. The impact of copper impregnated wound dressings on surgical site infection following caesarean section: a double blind randomised controlled study[J]. Eur J Obstet Gynecol Reprod Biol, 2020, 251: 83-88.
doi: S0301-2115(20)30267-0 pmid: 32502772
[42] JAGANATHAN S K, MANI M P. Electrospun polyurethane nanofibrous composite impregnated with metallic copper for wound-healing application[J]. Biotech, 2018, 8(8): 327-333.
[43] AGREN M S, MIRASTSCHIJSKI U. The release of zinc ions from and cytocompatibility of two zinc oxide dressings[J]. Journal of Wound Care, 2004, 13: 367-369.
pmid: 15517745
[44] 郭振荣, 李利根, 赵霖, 等. 锌营养状态对烧伤创面修复的实验研究[J]. 中国临床营养杂志, 2001, 9(12): 242-244.
GUO Zhenrong, LI Ligen, ZHAO Lin, et al. Experimental study on the effect of zinc nutritive status on repair of burn wound[J]. Chinese Journal of Clinical Nutrition, 2001, 9(12): 242-244.
[45] SEGAL H C, HUNT B J, GILDING K. The effects of alginate and non-alginate wound dressings on blood coagulation and platelet activation[J]. Journal of Biomaterials Applications, 1998, 12(3): 249-257.
pmid: 9493071
[46] HU G F. Copper stimulates proliferation of human endothelial cells under culture[J]. J Cell Biochem, 1998, 69:326-335.
doi: 10.1002/(sici)1097-4644(19980601)69:3<326::aid-jcb10>3.0.co;2-a pmid: 9581871
[47] PICKART L, FREEDMAN J H, LOKER W J, et al. Growth-modulating plasma tripeptide may function by facilitating copper uptake into cells[J]. Nature, 1980, 288: 715-717.
doi: 10.1038/288715a0
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