纺织学报 ›› 2023, Vol. 44 ›› Issue (11): 216-224.doi: 10.13475/j.fzxb.20220505402

• 综合述评 • 上一篇    下一篇

基于表面增强拉曼光谱的纳米纤维基生物传感器的研究进展

徐志豪1,2, 徐丹瑶1,2, 李彦1,2(), 王璐1,2   

  1. 1.东华大学 纺织学院, 上海 201620
    2.东华大学 纺织面料技术教育部重点实验室, 上海 201620
  • 收稿日期:2022-05-17 修回日期:2023-04-20 出版日期:2023-11-15 发布日期:2023-12-25
  • 通讯作者: 李彦(1987—),女,副教授,博士。主要研究方向为纳米纤维基生物纺织材料的功能设计及应用。E-mail:yanli@dhu.edu.cn。
  • 作者简介:徐志豪(1999—),男,硕士生。主要研究方向为医用纺织材料的设计与制备。
  • 基金资助:
    中央高校基本科研业务费专项资金资助项目(2232022G-01);高等学校学科创新引智计划项目(BP0719035)

Research progress in nanofiber-based biosensors based on surface enhanced Raman spectroscopy

XU Zhihao1,2, XU Danyao1,2, LI Yan1,2(), WANG Lu1,2   

  1. 1. College of Textiles, Donghua University, Shanghai 201620, China
    2. Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China
  • Received:2022-05-17 Revised:2023-04-20 Published:2023-11-15 Online:2023-12-25

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摘要：

为促进纳米纤维基表面增强拉曼光谱(SERS)传感器在生物医用领域的开发及应用,介绍了纳米纤维基SERS基底的组成与性能评价指标,纳米纤维基底的构建方法及其性能影响因素。通过总结纳米纤维基SERS基底的构建方法,阐述了纳米纤维与等离子体材料原位组装和后组装的2种策略,进一步探究了纳米纤维种类及其形貌等对柔性SERS传感性能的影响机制。最后展示了纳米纤维基SERS基底在生物医用领域的应用,根据生物医用领域SERS传感器的性能要求对其未来发展趋势进行展望,以期为制备高性能的纳米纤维基柔性SERS基底及拓宽其实际应用提供一定参考。

关键词: 表面增强拉曼光谱, 纳米纤维, 静电纺丝, 纳米颗粒, 生物医用, 生物传感器

Abstract:

Significance Surface enhanced Raman spectroscopy (SERS) sensors are usually constructed from active elements and substrate materials, which have the advantages of simple operation and rapid monitoring. Electrospun nanofibers have unique three-dimensional curved channel, large specific surface area, high porosity and controllable stacking density. It not only provides a large number of sites for the loading of nanoparticles, but also facilitates the capture and transport of molecules to be tested, thereby enhancing SERS signals and improving detection sensitivity. Compared with the rigid detection substrate, the flexible substrate represented by electrospinning nanofibers can provide a more flexible detection process in complex environments, so it has great application potential. In order to promote the development and application of nanofiber-based SERS sensors in the biomedical field, it is important to explore the factors that affect the SERS sensing performance of nanofibers.

Progress The composition and performance evaluation index of nanofiber-based SERS substrate, the construction method of nanofiber substrate, and its performance influencing factors are introduced. By summarizing the construction methods of nanofiber-based SERS substrates, two strategies of in-situ assembly and post-assembly of nanofibers and plasma materials are described. The SERS substrate prepared by in-situ assembly has good stability because most of the nanoparticles exist inside the fibers, but the detection sensitivity is slightly poor. The SERS substrate prepared by post-assembly is more easily combined with the molecule to be tested because the nanoparticles are distributed on the surface of the fiber, and the sensitivity is higher. The mechanism of the influence of the type and morphology of the nanofibers on the flexible SERS sensing performance was further explored. The difference in the hydrophilicity and hydrophobicity of the fibers and their morphology will affect the interaction with the nanoparticles and the molecules to be tested, thereby affecting the detection performance of the substrate. Finally, the application of nanofiber-based SERS substrate in the biomedical field is demonstrated, including for body fluid testing, determining the patient's health status and diagnosing related diseases, and in situ detection of bacteria.

Conclusion and Prospect Due to the limitations of rigid substrates in use, the research on flexible SERS substrates is increasing. Among them, nanofiber-based flexible SERS substrates with a three-dimensional network structure have great application prospects in real life. At present, much research effort was made on nanofiber-based flexible SERS substrate, but the development and application in the biomedical field are still insufficient. The complex physiological environment of the human body puts forward high requirements for the detection performance of SERS substrate. SERS substrate needs to have good anti-interference performance and stability on the premise of ensuring sensitivity. By revealing the performance influence mechanism of nanofiber-based SERS substrate and according to the performance requirements of SERS sensors in the biomedical field, the future development trend of nanofiber-based SERS substrate is prospected, with a view to providing some reference for how to prepare high-performance nanofiber-based flexible SERS substrate and broaden its practical application. It is hoped that nanofiber-based SERS substrates would be widely applied for human health life monitoring and disease diagnosis in vitro and in vivo.

Key words: surface enhanced Raman spectroscopy, nanofiber, electrospinning, nanoparticle, biomedical, biosensor

中图分类号: 

  • TS101.4

图1

SERS中电磁和化学增强机制示意图"

图2

高灵敏度三维多孔AgNPs/PVA/Ag纳米纤维SERS基底的合成过程示意图"

图3

SERS活性Au/TPU静电纺可穿戴汗液pH值传感器制造和应用示意图"

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