Abstract: The effect of epoxy crosslinking agent EH on the color fixation of cotton directly dyed with natural brazilwood dye was analyzed by K/S value,FT-IR,X-ray,DSCTGA and acid-dissolving rate testing,and reaction kinetics experiment was performed using hematein as a substitution for brazilwood dye.The results showed that under experiment conditions the reaction between hematein and epoxy crosslinking agent follows pseudo-first-order kinetics,and under alkaline condition epoxy crosslinking agent reacts simultaneously with both the hydroxy groups of hematein and cellulose.The cross linking reaction with cellulose mostly occurs in the amorphous region.