Abstract:

A rapid method was developed to determine the multi-residues of alkylphenols and alkylphenol polyethoxylates in the down and feather by high performance liquid chromatography. The samples could be extracted with methanol by accelerated solvent extractor (ASE). Then, the collected solution was analysed by HPLC with fluorescence detector (FLD) and quantified by external standard method. The Sinochrom ODS-BP column was selected，and the mixture of methanol-acentrile-water（81︰6︰13，V︰V ）was used as the HPLC mobile phase. The excitation wavelengh and the emission wavelengh were set at 230nm and 296nm, respectively. The result shows that the alkylphenols and alkylphenol polyethoxylates in the down and feather were separated efficiently from impurity in high sensitivity and reproducibility by HPLC-FLD.