Abstract:

For screening and obtaining microbial strains for biological degumming of ramie, 38 microbial strains, including 21 bacteria, 13 fungi and 4 actinomycetes were isolated from randomly selected putrid ramie by enrichment cultivation in combination with diluted coating. Identification of the strains was performed by morphology observation, physiological and biochemical reactions, and sequence analysis of 16S rRNA. Through screening with a method of transparent zones, 9 microbial strains were obtained，which had high and stable degradation ability to gum. Among them, the bacteria were indentified as Bacillus, Acinetobacter, Paenibacillus and Burkholderia. The degumming effects of the strains indicated that the bacillus subtilis strain isolated from putrid ramie is the best, with the residual gum content only 13.82%.