Alcalase水解丝素蛋白的特性

纺织学报 ›› 2007, Vol. 28 ›› Issue (5): 13-17.

Alcalase水解丝素蛋白的特性

周凤娟¹;许时婴²;杨瑞金²;王璋²

1.天津大学农业与生物工程学院天津300072;2.江南大学食品学院江苏无锡214036

收稿日期:2006-09-05 修回日期:2007-01-12 出版日期:2007-05-15 发布日期:2007-05-15

Properties of Alcalase hydrolyzing silk fibroin

ZHOU Fengjuan;XU Shiying;YANG Ruijin;WANG Zhang

1.College of Agriculture and Bioengineering;Tianjin University;Tianjin 300072;China;2.College of Food Science and Technology;Southern Yangtze

Received:2006-09-05 Revised:2007-01-12 Online:2007-05-15 Published:2007-05-15

摘要/Abstract

摘要： 研究了Alcalase水解丝素蛋白的特性。结果表明,Alcalase水解丝素蛋白的最佳条件是:丝素蛋白质量浓度50 mg/mL,pH值9.0,温度60℃;丝素肽中主要氨基酸(甘氨酸、丙氨酸、丝氨酸和酪氨酸)的含量之和占其氨基酸总量的85%左右;水解度为17%的丝素蛋白水解产物中主要组分的相对分子质量分别为249 Da和762 Da,含量分别约占72.9%和15.5%,水解度为21%的丝素蛋白水解产物中主要组分的相对分子质量分别为209 Da和668 Da,含量分别约占84.4%和7.2%。

Abstract: The properties of Alcalase hydrolyzing silk fibroin were studied.The results showed that the optimum conditions for Alcalase were 50 mg/mL substrate mass concentration,pH 9.0 and 60 ℃.The main amino acids of silk fibroin peptide were glycine,alanine,serine and tyrosine with a total amount of approximately 85%.The relative molecular weights of the main components of silk fibroin hydrolysate with DH 17% were 249 Da and 762 Da respectively,the contents about 72.9% and 15.5% respectively.The relative molecular weights of the main components of silk fibroin hydrolysate with DH 21% were 209 Da and 668 Da respectively,the contents about 84.4% and 7.2% respectively.

周凤娟;许时婴;杨瑞金;王璋. Alcalase水解丝素蛋白的特性[J]. 纺织学报, 2007, 28(5): 13-17.

ZHOU Fengjuan;XU Shiying;YANG Ruijin;WANG Zhang. Properties of Alcalase hydrolyzing silk fibroin[J]. JOURNAL OF TEXTILE RESEARCH, 2007, 28(5): 13-17.

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