Abstract: Octylphenol ethoxylates are one of important Alkylphenol ethoxylates, widely used in textile, leather and daily life. Recently, the fate and effects of OPEO in the environment have received considerable attention because their bio-degradation products have been proved to have estrogen-mimicking effects to nature receptor. In this paper, through the study OPEO on adsorption of silk fiber, in order to give a theoretical guidance mentioned for quarantine inspection. The results showed the adsorption isotherms fitted Langmuir model. The adsorption process could be well described using pseudo-second-order kinetics, and initial OPEO concentrations are larger, the adsorption rate constants are smaller and that reach to balance longer. The higher the temperature OPEO solution, the adsorption rate constants is larger. The pellet diffusion is a main factor in silk fiber adsorption of OPEO, but is not the only factor. The adsorption activation energy is 34.33kJ/mol in silk fiber of OPEO, which is far less than that of chemical adsorption required activation 83.72kJ/mol. It indicates that it belongs to physics adsorption.