脂肪醇聚氧乙烯醚对细胞的增殖抑制影响

纺织学报 ›› 2011, Vol. 32 ›› Issue (10): 79-0.

脂肪醇聚氧乙烯醚对细胞的增殖抑制影响

陈晓伟,王晓宁,龚龑,廖青

北京服装学院

收稿日期:2010-09-30 修回日期:2011-06-30 出版日期:2011-10-15 发布日期:2011-10-24
通讯作者: 廖青 E-mail:clylq@bift.edu.cn
基金资助:
市级

Growth Inhibition Effects of Ethoxylate Fatty alcohol on Chinese hamster CHO cells and HeLa cells

Received:2010-09-30 Revised:2011-06-30 Online:2011-10-15 Published:2011-10-24

摘要/Abstract

摘要： 本文采用噻唑蓝（MTT）比色法对脂肪醇聚氧乙烯醚系列表面活性剂对中华仓鼠卵巢CHO细胞及人宫颈癌HeLa细胞增殖抑制情况进行了研究，结果表明：随着环氧乙烷（EO）数量的增加脂肪醇聚氧乙烯醚对CHO细胞及HeLa细胞增殖抑制率均逐渐减小，即AEO5对两种细胞增殖抑制率最大，AEO9次之，AEO15最小； AEO5浓度大于0.05μL/mL时，细胞增殖抑制率趋于平缓。AEO9浓度大于0.075μL/mL时，细胞增殖抑制率趋于平缓。AEO15浓度为0. 1μL/mL时，细胞增殖抑制率仍呈现上升趋势。当AEO5、AEO9浓度不大于0.005μL/mL时，对CHO细胞及HeLa细胞增殖无明显抑制。当AEO15浓度不大于0.01μL/mL时，对CHO细胞及HeLa细胞增殖无明显抑制。

Abstract: In this article, MTT assay is adopted to study three different kinds of ethoxylate fatty alcohol on Chinese Hamster Ovary（CHO）cells and Hela cells. The results indicate that the reproduction restraining effect of fatty alcohol ethoxylates both on CHO cells and Hela cells gradually decreased as the numbers of EO increased. In the other words, AEO5 shows the maximum inhibitory rates, followed is AEO9, and the minimum is AEO15. When the concentration of AEO5 is more than 0.05μL/mL and AEO9 is more than 0.075μL/mL, both their trend toward gentle inclined. When the concentration of AEO15 is 0. 1μL/mL, it’s growth inhibition rate still shows upward tendency. When the concentration of AEO5 and AEO9 are less than 0.005μL/mL, their reproduction restraining effects on CHO cells and Hela cells are unconspicuous. When the concentration of AEO15 is no more than 0.01μL/mL, its reproduction restraining effect on CHO cells and Hela cells is obsolete.

陈晓伟王晓宁龚龑廖青. 脂肪醇聚氧乙烯醚对细胞的增殖抑制影响[J]. 纺织学报, 2011, 32(10): 79-0.

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