黄多孔菌产漆酶最佳培养条件的优化

纺织学报 ›› 2012, Vol. 33 ›› Issue (2): 63-67.

黄多孔菌产漆酶最佳培养条件的优化

徐雪霏，毕云枫，蒋海芹，沈明浩

吉林农业大学食品科学与工程学院

收稿日期:2011-04-27 修回日期:2011-08-21 出版日期:2012-02-15 发布日期:2012-02-15
通讯作者: 沈明浩 E-mail:shenmh2003@yahoo.com.cn
基金资助:
省级

Optimization of culture conditions for laccase production from Polyporus elegans Fr.

XU Xue-Fei, BI Yun-Feng, JIANG Hai-Qin, SHEN Ming-Hao

Received:2011-04-27 Revised:2011-08-21 Online:2012-02-15 Published:2012-02-15

摘要/Abstract

摘要： 采用Plackett-Burman设计法,对影响黄多孔菌生产漆酶的9个因素进行了筛选。结果表明，影响该菌产生漆酶的主要营养因素为碳氮比、铜离子浓度和pH。在此基础上,采用响应面法对其中3个显著因子的最佳水平范围进行研究,利用统计软件Design-Expert进行二次回归分析得知, 碳氮比、铜离子浓度和pH分别为4:1、0.1 mmol/L和5.5时,漆酶产量从178 U/mL提高到446 U/mL。

Abstract: The 9 factors that influenced laccase production from Polyporus elegans Fr. was studied using Plackett-Burman design. The result showed that the main nutrition factors was C/N ratio, the concentration of Cu2+ and pH. On that basis，response surface analysis were adopted to investigate the optimal levels of the 3 main factors. The result was undertaked the second regression analysis using Design-Expert software and showed that the laccase activity was 446U/mL(178U/mL before optimization) when the C/N ratio, the concentration of Cu2+ and pH was 4:1, 0.1 mmol/L and 5.5 respectively.

徐雪霏, 毕云枫, 蒋海芹, 沈明浩. 黄多孔菌产漆酶最佳培养条件的优化[J]. 纺织学报, 2012, 33(2): 63-67.

XU Xue-Fei, BI Yun-Feng, JIANG Hai-Qin, SHEN Ming-Hao. Optimization of culture conditions for laccase production from Polyporus elegans Fr.[J]. JOURNAL OF TEXTILE RESEARCH, 2012, 33(2): 63-67.

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