23种致癌芳香胺的显色法测定

纺织学报 ›› 2012, Vol. 33 ›› Issue (12): 58-63.

23种致癌芳香胺的显色法测定

叶曦雯¹,李引^1，2,牛增元¹,张清智¹,高永刚¹

1. 山东出入境检验检疫局
2. 青岛科技大学环境与安全工程学院

收稿日期:2011-12-21 修回日期:2012-06-07 出版日期:2012-12-15 发布日期:2012-12-20
通讯作者: 叶曦雯 E-mail:ye.xiwen@163.com
基金资助:
2006年度国家质量监督检验检疫总局科研项目

Chromogenic method for determination of 23 kinds of carcinogenic aromatic amines

Received:2011-12-21 Revised:2012-06-07 Online:2012-12-15 Published:2012-12-20

摘要/Abstract

摘要： 为了减少禁用芳香胺检测过程中对大型色谱仪器的依赖，提高检测速度，降低检测成本，建立了23种致癌芳香胺的邻甲氧基苯酚显色测定方法。根据芳香胺的结构特征，引入重氮化-偶合反应机理，有效考察、优化了23种芳香胺重氮化反应和偶合反应过程中的各项条件，综合考虑，最佳的反应条件是：在室温下，23种芳香胺和亚硝酸钠在pH值为2.56时反应生成重氮盐，加入氨基磺酸铵除去多余的亚硝酸钠后，加入邻甲氧基苯酚与重氮盐偶联显色，显色的最佳pH值范围为10-11，显色反应可以迅速完成，生成的偶氮化合物可稳定180min以上。方法的线性范围为1～50mg/L 。该方法操作简单易行，可有效地应用于纺织品中23种致癌芳香胺的同时定性筛选检测。

关键词: 致癌芳香胺, 显色法, 重氮化偶合反应, 邻甲氧基苯酚

Abstract: In order to improve the detection speed and reduce the cost of testing,a chromogenic reaction of 23 carcinogenic aromatic amines with guaiacol ,which not completely depend on large scale chromatography instrument ,was developed . According to the structural features of aromatic amines and mechanism of diazo-coupling reaction, all terms of diazo-coupling reaction process were studied and optimized effectively. The optimal conditions were obtained as follows: at room temperature, pH of diazo-reaction was 2.56, pH of coupling reaction was 10 -11, diazo-reaction time was 10 min, diazo-reaction products were stable during 180min. Linearity was excellent in the concentration range of 1-50mg/L. This method could be effectively applied to textiles in qualitative screening test of 23 carcinogenic aromatic amines.

Key words: carcinogenic aromatic amines, chromogenic method, diazo-coupling reaction, guaiacol

叶曦雯李引牛增元张清智高永刚. 23种致癌芳香胺的显色法测定[J]. 纺织学报, 2012, 33(12): 58-63.

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