碳源对克氏菌合成PTT原料PDO的影响及对DhaB的表达调控

纺织学报 ›› 2014, Vol. 35 ›› Issue (8): 144-0.

碳源对克氏菌合成PTT原料PDO的影响及对DhaB的表达调控

季广建^1，2,聂玲燕^1，2,陆信曜^1，2,宗红^1，2,宋健^1，3,方慧英^1，2,诸葛斌^1，2

1. 江南大学工业微生物研究中心
2. 江南大学工业生物技术教育部重点实验室
3. 江南大学化学与材料工程学院

收稿日期:2014-04-02 修回日期:2014-04-18 出版日期:2014-08-15 发布日期:2014-08-15
通讯作者: 诸葛斌 E-mail:bzhuge@163.com
基金资助:
国家高技术研究发展计划（863计划）重点项目

Influence of carbon sources on biosynthesis of 1,3-propandiol, raw material of PTT, and its regulation to DhaB in Klebsiella pneumonia

Received:2014-04-02 Revised:2014-04-18 Online:2014-08-15 Published:2014-08-15

摘要/Abstract

摘要： 为提高1,3-丙二醇（PDO）产量，加快聚对苯二甲酸丙二醇酯（PTT）的产业化应用。考察了不同碳源对克雷伯氏菌合成PDO过程中碳流的影响和对关键酶DhaB的表达调控。结果表明，克雷伯氏菌不能直接利用葡萄糖合成PDO；以甘油为单一碳源时，PDO的产量仅为9 g/L，同时细胞生长受到一定抑制；而在甘油为底物的情况下，添加5 g/L的葡萄糖能够使菌体生物量提高66.7%、dhaB的转录上调20%、DhaB酶活提高64%，同时PDO产量提高1.5倍。上述结果表明混合碳源策略能够激活克雷伯氏菌PDO合成途径关键酶DhaB的表达，提高PDO的产量。

关键词: 聚对苯二甲酸丙二醇酯, 1, 3-丙二醇, 克雷伯氏菌, 碳源, 调控

Abstract: The yield of 1,3-propandiol （PDO）is a bottleneck of the industrial application of polytrimethylene-tereph-thalate （PTT）. In order to increase the titer of PDO, the influences of different carbon sources to the carbon fluxes and the transcription and activity of key enzyme DhaB during the biosynthesis of PDO by Klebsiella pneumonia ZG25 were studied. When using glucose as the sole carbon source, the biomass of K. pneumonia ZG25 was highest but the PDO was not detected in the medium. The PDO yield of K. pneumonia ZG25 by using glycerol as the sole carbon source achieved 9 g/L. Compared with the fermentation by using glycerol as the sole carbon source, the biomass and the yield of PDO were increased by 66.7% and 150% respectively, when 5 g/L glucose was applied as supplementary carbon source. Further analysis exhibited that the mRNA level of dhaB and the activity of DhaB were also increased by 20% and 64% respectively. These results suggest that the mixed carbon sources strategy activate the transcription of dhaB, enhance the activity of DhaB and strengthen the synthesis of PDO by K. pneumonia.

Key words: polytrimethylene tereph thalate, 1,3-propandiol, klebsiella pneumonia, carbon source, regulation

季广建聂玲燕陆信曜宗红宋健方慧英诸葛斌. 碳源对克氏菌合成PTT原料PDO的影响及对DhaB的表达调控[J]. 纺织学报, 2014, 35(8): 144-0.

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