Abstract: Regarding the White Rot Fungus Pleurotus djamor as the templet,the complete sequence of Laccase Gf-lac1's gene can be got through the RT-PCR. In this way,the expression vector of Pichia pastoris named pPICZB-Gf-Lac1 is built and it is imported into Pichia pastoris by electrotransformation .What's more,part of masculine transformants called PCR turns out that the sequence CDS of Gf-Lac1 has been integrated in the Pichia pastoris chromosome.Besides,after training in the shake-flask liquid, the protein with higher pastoris engineering strains are selected. Furthermore, the vitality of laccase is reached 53U/L, and the study that laccase restructured is influenced dyeware bleaching has been researched further. The result turns out that the bleaching rate -restructring laccase to Alizarin Red Anthraquinone can be reached 53.6%; on the other hand, the bleaching rates of Heterocyclic Neutral Red, Methyl Orange Azo and Triphenylmethane Malachite Green are lower than Alizarin Red, which are namely 15.5%, 15.1% and 0.23%. In the words, it shows that the efficiency of restrucruring laccase to Alizarin Red possesses larger potential applications, thus it owns wider applied prospects in wastewater treatment.