纺织学报 ›› 2015, Vol. 36 ›› Issue (12): 16-19.

• 纤维材料 • 上一篇    下一篇

苎麻水解液中单糖类组分的柱前衍生-高效液相色谱法检测

  

  • 收稿日期:2015-02-15 修回日期:2015-08-07 出版日期:2015-12-15 发布日期:2015-12-10

Constituents of monosaccharides in hydrolysed ramie detected by high performance liquid chromatography with pre-column derivatization

  • Received:2015-02-15 Revised:2015-08-07 Online:2015-12-15 Published:2015-12-10

摘要:

为加速苎麻纤维的合理开发和应用,建立了一套苎麻单糖类组成的分析方法。利用三氟乙酸水解苎麻,1-苯基-3-甲基-5-吡唑啉酮柱前衍生水解产物,采用C18色谱分析柱和紫外检测器检测苎麻水解产物中的单糖类组成。结果表明:苎麻水解率可达90%,水解产物包括葡萄糖、鼠李糖、半乳糖醛酸、甘露糖、半乳糖、木糖等已知组分及少量未知组分,其中葡萄糖为主要组分,占已知单糖类总量的83.86%~86.54%;各单糖类组分的标准曲线线性相关系数为0.9996~0.9999,精密度均在2.02%以下,加样回收率为96.53%~102.76%。该方法能满足仪器检测要求,适用于分析苎麻水解产物中的单糖类组分。

Abstract:

To improve the exploitation and application of ramie fiber, a set of method for the composition analysis of ramie single sugars was established. The high performance liquid chromatography (HPLC) coupling with pre-column derivatization by 1-phenyl-3-methyl-5-pyrazolone (PMP) was used to analyze the monosaccharides in ramie. The samples were hydrolyzed by trifluoroacetic acid, then analyzed by HPLC with C18 column and ultraviolet detector. All monosaccharides possessed good linear relationship with the precision below 2.02% and recoveries between 96.53% and 102.76%. The results showed that the hydrolysis rate of ramie was 90%, the main monosaccharides were mannose, rhamnose, galacturonic acid, glucose, galactose, xylose and few unknown monosaccharides. The glucose was the main constituent, with the proportion of 83.86%~86.54% of the known monosaccharides. The HPLC method coupling with pre-column derivatization by PMP can meet the detection requirements of instrument, and is suitable for the analysis of the hydrolysed monosaccharides in ramie.

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