再生纤维素纳米纤维膜的制备及其蛋白质分离性能

纺织学报 ›› 2016, Vol. 37 ›› Issue (12): 12-17.

再生纤维素纳米纤维膜的制备及其蛋白质分离性能

收稿日期:2015-12-31 修回日期:2016-08-24 出版日期:2016-12-15 发布日期:2016-12-21

Preparation and protein separation properties of regenerated cellulose nanofiber membrane

Received:2015-12-31 Revised:2016-08-24 Online:2016-12-15 Published:2016-12-21

摘要/Abstract

摘要：

为将纳米纤维膜应用于蛋白质分离处理，用静电纺丝和化学改性方法制备聚丙烯腈/再生纤维素（PAN ∕ RC）复合纳米纤维膜，通过扫描电镜、红外光谱、比表面积及孔径分析等对制备的复合纳米纤维膜进行了表征，并将制备的再生纤维素复合纳米纤维膜作为分离层，构建膜分离系统并分离纯化血清白蛋白，通过调节操作压力和过滤时间等影响因素，确定其分离纯化过程的最佳条件。研究结果表明：在操作压力为0.10 MPa、过滤时间为1.5h条件下，再生纤维素复合纳米纤维膜对蛋白质的截留率达到80.04%，膜通量达到1.85L ∕ （m² ?min），与商用聚醚砜超滤膜相比，在截留率差异不大的情况下，膜通量有了数倍的提升；同时再生纤维素复合纳米纤维膜具有优异的重复使用能力，并在使用的过程中保持良好的纳米纤维形态结构。

关键词: 静电纺丝, 再生纤维素纳米纤维, 蛋白质分离, 膜通量

Abstract:

Polyacrylonitrile/ regenerated cellulose (PAN / RC) composite nanofibers membrane was prepared by electrospinning and chemical modification for the separation and purification of serum albumin. Composite nanofiberous membrane was characterized by scanning electron microscopy, infrared spectroscopy, specific surface area and pore size analysis. The membrane separation system was assembled by the PAN / RC composite nanofibers membrane, which was overlaid to form the reaction layer. The optimal condition of the protein separation were analyzed by adjusting the operating pressure, filtration time and the pH value. Results showed that the rejection rate of serum albumin and flux of membrane could reach 80.04 % and 1.85 L/(m² • min), respectively, under the following conditions: the operating pressure was 0.10 MPa, and the processing time was 1.5 h. Compared with the polyether sulphone commercial ultrafiltration membrane, the rejection rate decreased slightly, while the transport flux improved by several times. At the same time, the experimental results indicated that the regenerated cellulose nanofibrous membrane possessed excellent reusability. Additional, the regenerated cellulose nanofibrous membrane could well retain the nanofibrous morphological structure after the separation of serum albumin for several times in aqueous environment.

Key words: electrospinning, regenerated cellulose nanofiber, separation of protein, flux

凤权武丁胜桓珊杨子龙应志祥. 再生纤维素纳米纤维膜的制备及其蛋白质分离性能[J]. 纺织学报, 2016, 37(12): 12-17.

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