Abstract:

In order to improve the imteraction effect of protease and wool, reduce the damage to wool and shorten the time of enzyme treatment, the catalytic effectiveness of protease in the presence of activator and the reduction rate between activator and wool were strdied to verify the feasibility of biological enzyme synergistic catalysis system composed of protease and activator. The synergistic effect of protease and activator in the enzyme catalytic system was proved to promote the hydrolysis rate of wool keratin significantly, by comparing the reduction rate, the change in protein content in liquid and scale image. The analysis results of the dyeing properties, amino acid composition changes. Allworden reaction and X-ray photoelectron spectroscopy show that the mechanism of biological enzyme synergistic catalytic system is the synergistic effect of protease and activator. Activator acts on the disulfide bond and protease on the amide bond, resulting in the peeling of wool scales and effectively.

Key words: biological enzyme, synergistic catalysis, wool, scale, keratin, shrink resistant treatment