Journal of Textile Research ›› 2019, Vol. 40 ›› Issue (03): 1-7.doi: 10.13475/j.fzxb.20180400207

• Fiber Materials •     Next Articles

Preparation of wool keratin solution by reducing agent-formic acid process

LI Bo1, YAO Jinbo2,3(), NIU Jiarong1, WANG Le1, FENG Mao1, SUN Yanli1   

  1. 1. School of Textile Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China
    2. College of Chemistry and Chemical Engineering, Wuhan Textile University, Wuhan, Hubei 430073, China
    3. Hubei Key Laboratory of Biomass and Eco-Dyeing & Finishing, Wuhan Textile University, Wuhan, Hubei 430073, China
  • Received:2018-04-02 Revised:2018-11-30 Online:2019-03-15 Published:2019-03-15
  • Contact: YAO Jinbo E-mail:jinboyao1964@wtu.edu.cn

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Abstract:

In order to obtain the wool keratin solution with high fiber dissolution ratio and large protein molecular weight, the dissolution method of wool fibers was studied and optimized. On the basis of using organic phosphonic compounds (LKS-610) to effectively cleave the disulfide bonds in fibers, the dissolution state of pre-treated wool fibers in formic acid solution was studied. The fibers dissolution ratio, keratin solution viscosity and protein molecular weight distribution were tested as evaluation criteria, the optimal process for preparing keratin solution by using reducing agent-formic acid method was obtained, and the stability performance of keratin solution was also analyzed. The optimal dissolving process of wool fibers is that the mass of the pretreated wool fibers is 5 g, the volume of formic acid is 100 mL, the temperature is 50 ℃ and time is 5 h. Under the conditions, the dissolution ratio is about 65% and the molecular weights of keratin are distributed at 40-50 ku, 26 ku and 14.4 ku. In addition, the keratin solution shows good stability at normal temperature.

Key words: wool, keratin solution, reducing agent, formic acid, protein molecular weight

CLC Number: 

  • TS195.5

Tab.1

Amino acid content test results of wool fiber samples"

氨基酸
名称		 未处理羊毛 处理30 min羊毛 处理60 min羊毛
质量/
(g·(100 g)-1)		 占氨基酸
百分比/%		 质量/
(g·(100 g)-1)		 占氨基酸
百分比/%		 质量/
(g·(100 g)-1)		 占氨基酸
百分比/%
天门冬氨酸 5.164 2 6.26 5.427 2 7.46 5.283 8 7.59
苏氨酸 4.830 5 5.88 4.174 5 5.74 3.897 3 5.60
丝氨酸 7.030 1 8.52 5.970 2 8.21 5.603 0 8.05
谷氨酸 11.074 4 13.42 11.209 0 15.42 10.819 0 15.54
甘氨酸 4.407 4 5.34 3.896 1 5.36 3.645 3 5.24
丙氨酸 3.342 3 4.05 4.474 2 6.15 4.673 6 6.71
胱氨酸 8.325 0 10.09 1.123 7 1.55 0.732 3 1.05
缬氨酸 3.990 8 4.83 3.701 1 5.09 3.440 8 4.94
蛋氨酸 0.627 3 0.76 0.497 1 0.68 0.415 6 0.60
异亮氨酸 2.664 2 3.23 2.552 4 3.51 2.484 0 3.57
亮氨酸 6.958 6 8.43 7.016 1 9.65 6.907 8 9.92
酪氨酸 4.257 3 5.16 3.748 4 5.16 3.572 4 5.13
苯丙氨酸 3.173 6 3.84 2.604 7 3.58 2.631 0 3.78
赖氨酸 2.865 8 3.47 2.926 5 4.03 2.957 0 4.25
NH3(色氨酸分解) 1.241 7 1.50 1.179 5 1.62 1.130 5 1.62
组氨酸 0.941 7 1.14 0.846 3 1.16 0.806 4 1.16
精氨酸 7.870 2 9.53 7.990 8 10.99 7.777 1 11.17
脯氨酸 3.775 5 4.57 3.369 1 4.63 2.825 1 4.06
氨基酸总量 82.545 3 100.00 72.706 8 100.00 69.602 1 100.00

Fig.1

Dissolving state of untreated wool fibers in formic acid solution under different dissolution time"

Fig.2

Dissolving state of LKS-610 treated wool fibers in formic acid solution under different dissolution time"

Tab.2

Dissolution rate and viscosity changes of wool with different mass dissolved in formic acid"

溶液中羊毛质量/g 纤维溶解率/% 溶液黏度/(Pa·s)
3 72.3 0.023
4 70.6 0.040
5 66.7 0.054
6 — —

Fig.3

SDS-PAGE gel electrophoretogram of samples with different disolved mass fraction"

Tab.3

Dissolution rate and viscosity change of samples at different dissolved temperatures"

溶解温度/ ℃ 纤维溶解率/% 溶液黏度/(Pa·s)
20 52.9 0.015
30 54.7 0.052
40 56.3 0.045
50 63.5 0.066
60 67.2 0.048
70 72.9 0.030
80 80.6 0.014

Fig.4

SDS-PAGE gel electrophoretogram of samples at different dissolved temperatures"

Tab.4

Dissolution rate and viscosity change of samples under different dissolving time"

纤维溶解时间/h 纤维溶解率/% 溶液黏度/(Pa·s)
3 47.9 0.047
4 52.7 0.050
5 62.5 0.060
6 68.9 0.039

Fig.5

SDS-PAGE gel electrophoretogram of samples under different dissolved time"

Fig.6

Molecular weight distribution of keratin solution after standing at room temperature for different time periods"

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