Journal of Textile Research ›› 2019, Vol. 40 ›› Issue (06): 73-78.doi: 10.13475/j.fzxb.20180506506

• Dyeing and Finishing & Chemicals • Previous Articles     Next Articles

Wool anti-felting treatment based on modified protease

MEI Jingxia, ZHANG Nan, WANG Qiang(), YUAN Jiugang, FAN Xuerong   

  1. Key Laboratory of Eco-Textiles(Jiangnan University), Ministry of Education, Wuxi, Jiangsu 214122, China
  • Received:2018-05-25 Revised:2019-03-09 Online:2019-06-15 Published:2019-06-25
  • Contact: WANG Qiang E-mail:qiangwang@jiangnan.edu.cn

RichHTML

12

PDF (PC)

73

Abstract:

Aiming at the severe damage to wool fiber and weak hydrolysis of keratin in scale caused by protease during wool fabric anti-felting finishing, protease was chemically modified with HOOC-PEG-COOH and L-cysteine to enlarge its volume so as to limit its hydrolysis to the surface of fiber and to be endued with the capability of reducing disulfide bonds in keratin. The anti-felting effect, the surface morphology, wetting properties and the degree of enzymolysis on wool fabric finished with the modified protease were further investigated by the dimensional stability to felting, breaking strength, SEM, contact angle, Allworden reaction and methylene blue coloration. The results show that the dimensional stability to felting and strength loss of wool fabrics finished with modified protease are superior to those of native protease. In addition, the hydrolysis of modified protease is limited to the scale layer of wool, and the modified protease is more harmful than unmodified protease for wool scale.

Key words: wool, protease, anti-felting finishing, chemical modification, scale, keratin

CLC Number: 

  • TS195.5

Fig.1

Dimensional stability to felting(a) and strength loss(b) of wool fabrics treated with different proteases after different treatment time"

Fig.2

Contact angles of wool fabrics at 100 s after different enzymatic treatments. (a) Raw wool; (b) Buffer treatment; (c) Savinase treatment; (d) L-cysteine/Savinase treatment; (e) SAV-PEG-L-cys treatment"

Fig.3

Allworden reactions of wool fibers after different enzymatic treatments (×400). (a) Raw wool; (b) Buffer treatment; (c) Savinase treatment; (d) L-cysteine/Savinase treatment; (e) SAV-PEG-L-cys treatment"

Fig.4

SEM images of wool fabrics after different enzymatic treatments for 3 h(×1 500). (a) Raw wool; (b) Buffer treatment; (c) Savinase treatment; (d) L-cysteine/Savinase treatment; (e) SAV-PEG-L-cys treatment"

Fig.5

Photographs of wool fibers after different enzymatic treatments (×400). (a) Raw wool; (b) Buffer treatment; (c) Savinase treatment; (d) L-cysteine/Savinase treatment; (e) SAV-PEG-L-cys treatment"

[1] 范雪荣, 黄庞惠, 王强. 羊毛的酶法防毡缩整理综述[J].针织工业, 2015(3):30-35.
FAN Xuerong, HUANG Panghui, WANG Qiang. Review of wool enzymatic anti-felting finishing[J]. Knitting Industries, 2015(3):30-35.
[2] SHEN J, SMITH E, CHIZYUKA M, et al. Development of durable shrink-resist coating of wool with sol-gel polymer processing[J]. Fibers and Polymers, 2017,18(9):1769-1779.
[3] SCHROEDER M, SCHWEITZER M, LENTING H B M, et al. Chemical modification of proteases for wool cuticle scale removal[J]. Biocatalysis and Biotransformation, 2009,22(5/6):299-305.
[4] SMITH E, SHEN J. Surface modification of wool with protease extracted polypeptides[J]. Journal of Biotechnology, 2011,156(2):134-140.
pmid: 21871933
[5] KAUR A, CHAKRABORTY J N, DUBEY K K. Enzymatic functionalization of wool for felting shrink-resistance[J]. Journal of Natural Fibers, 2016,13(4):437-450.
[6] LCHEZ V S, JOVANĆIĆ P, ERRA P. Influence of chitosan on the effects of proteases on wool fibers [J]. Fibers and Polymers, 2010,11(1):28-35.
[7] JUS S, SCHROEDER M, GUEBITZ G M, et al. The influence of enzymatic treatment on wool fibre properties using PEG-modified proteases[J]. Enzyme and Microbial Technology, 2007,40(7):1705-1711.
[8] 陈忍忍, 袁久刚, 余圆圆, 等. 壳聚糖修饰羊毛防毡缩用蛋白酶的制备及其酶学性质[J]. 食品与生物技术学报, 2012,31(5):63-68.
CHEN Renren, YUAN Jiugang, YU Yuanyuan, et al. Preparation of chitosan conjugated protease used for shrink resistance of wool and its properties[J]. Journal of Food Science and Biotechnology, 2012,31(5):63-68.
[9] 朱怡然, 范雪荣, 王强, 等. 右旋糖酐对Savinase蛋白酶的化学修饰及酶学性质研究[J]. 中国生物工程杂志, 2011,31(10):45-49.
ZHU Yiran, FAN Xuerong, WANG Qiang, et al. Chemical modification of Savinase by dextran and its properties[J]. China Biotechnology, 2011,31(10):45-49.
[10] MEI J, ZHANG N, YU Y, et al. A novel "trifunctional protease" with reducibility, hydrolysis, and localization used for wool anti-felting treatment[J]. Applied Microbiology & Biotechnology, 2018,102(21):9159-9170.
doi: 10.1007/s00253-018-9276-y pmid: 30083801
[11] KUZUHARA A, HORI T. Analysis of heterogeneous reaction between reducing agents and keratin fibers using Raman spectroscopy and microspectrophoto-metry[J]. Journal of Molecular Structure, 2013,1037:85-92.
[12] SHEN J, RUSHFORTH M, CAVACO-PAULO A, et al. Development and industrialisation of enzymatic shrink-resist process based on modified proteases for wool machine washability[J]. Enzyme and Microbial Technology, 2007,40(7):1656-1661.
[13] 黄庞慧, 王强, 董爱学, 等. 角质酶/角蛋白酶一浴法处理对羊毛性能的影响[J]. 生物学杂志, 2014,31(6):94-98.
HUANG Panghui, WANG Qiang, DONG Aixue, et al. Effects of cutinase-keratinase treatment in one bath on wool properties[J]. Journal of Biology, 2014,31(6):94-98.
[14] 刘建勇, 吴胜争, 赵笑康. 生物酶协同催化体系及其对羊毛纤维的作用机制[J]. 纺织学报, 2018,39(1):71-78.
LIU Jianyong, WU Shengzheng, ZHAO Xiaokang. Biological enzyme synergistic catalytic system and its mechanism on wool fiber[J]. Journal of Textile Research, 2018,39(1):71-78.
[1] SHAO Jinxin, ZHANG Baochang, CAO Jipeng. Fiber detection and recognition technology in cotton fiber carding process based on image processing and deep learning [J]. Journal of Textile Research, 2020, 41(07): 40-46.
[2] ZHANG Aidan, ZHOU Jiu. Grayscale simulation characteristics of jacquard fabric with cluster-dotted structure [J]. Journal of Textile Research, 2020, 41(03): 62-67.
[3] LOU Shaohong. Automatic generation of A-type skirt model based on AutoCAD parametric function [J]. Journal of Textile Research, 2020, 41(01): 131-138.
[4] CUI Guixin, DONG Yongchun, WANG Peng. Preparation and application properties of wool-Fe complex-based heterogeneous Fenton photocatalysts [J]. Journal of Textile Research, 2019, 40(12): 68-73.
[5] TU Li, MENG Jiaguang, LI Xin, LI Juanzi. Composition analysis and stripping process of waste wool/silk/cotton blended fabric [J]. Journal of Textile Research, 2019, 40(11): 75-80.
[6] HAN Ye, ZHANG Hui, ZHU Guoqing, WU Hailiang. Effect of polyethylene glycol on photocatalytic properties of polyethylene terephthalate fibers treated with titanium sulfate under hydrothermal conditions  [J]. Journal of Textile Research, 2019, 40(10): 33-41.
[7] WEI Lingli, ZOU Qinshan, WANG Lu, LUO Jing, XIA Xin. Preparation of fluorine-free water repellent treated wool/cashmere fabrics [J]. Journal of Textile Research, 2019, 40(09): 102-107.
[8] GAO Jing, WANG Lu. Influence of pretreatment process on superhydrophobic modification of wool/polyester fabric [J]. Journal of Textile Research, 2019, 40(09): 91-96.
[9] . Rapid detection of banned dye Acid Red 26 in pure wool thread based on oblique projection [J]. Journal of Textile Research, 2019, 40(08): 85-88.
[10] . Measurement for bending behavior by bowknot method [J]. Journal of Textile Research, 2019, 40(08): 35-39.
[11] . Preparation and properties of multifunctional composite conductive wool fabric [J]. Journal of Textile Research, 2019, 40(08): 117-123.
[12] BAI Gang, LIU Yanchun, QIAN Hongfei. Preparation of immobilized cross-linked protease aggregate and sanding effect thereof on silk fabric [J]. Journal of Textile Research, 2019, 40(07): 113-118.
[13] BAO Hong, XU Shui, ZHANG Xiaoning, CHENG Guotao, ZHU Yong. Cationization of Bombyx mori silk fibroin and effect there of on wool traits [J]. Journal of Textile Research, 2019, 40(07): 24-30.
[14] AN Fangfang, FANG Kuanjun, LIU Xiuming, CAI Yuqing, HAN Shuang, YANG Haizhen. Effect of wool fabric protease modification on droplet spreading and color performance [J]. Journal of Textile Research, 2019, 40(06): 58-63.
[15] LIU Shuping, LI Liang, LIU Rangtong, CUI Shizhong, WANG Yanting. Structure and properties of keratin film modified by carboxymethyl cellulose sodium [J]. Journal of Textile Research, 2019, 40(06): 14-19.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备14006648号
Copyright 2021 © Editorial office of Journal of Textile Research
Supported by Beijing Magtech Co.Ltd