Journal of Textile Research ›› 2019, Vol. 40 ›› Issue (07): 113-118.doi: 10.13475/j.fzxb.20180704906

• Dyeing and Finishing & Chemicals • Previous Articles     Next Articles

Preparation of immobilized cross-linked protease aggregate and sanding effect thereof on silk fabric

BAI Gang1,2, LIU Yanchun1,2, QIAN Hongfei1,2   

  1. 1. College of Textiles and Fashion, Shaoxing University, Shaoxing, Zhejiang 312000, China
    2. Zhejiang Key Laboratory of Clean Dyeing and Finishing Technology, Shaoxing, Zhejiang 312000, China
  • Received:2018-07-20 Revised:2019-04-18 Online:2019-07-15 Published:2019-07-25

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Abstract: Aim

ing at the obvious strength decrease and serious fading in silk sanding with conventional alkali agent, the immobilized cross-linked protease aggregates (ICLPAs) were used for sanding of silk fabrics. The preparation conditions of ICLPAs were optimized, and the stability and reusability of ICLPAs were investigated. The properties of ICLPAs-treated fabric were studied by testing KES fabric style, capillary effect, air permeability,moisture penetrability, thermal resistance, wet resistance, drapability and strength. The results show that the thermal stability, pH stability and reusablility of ICLPAs are all improved compared to free protease. The silk fibroin of the ICLPAs-treated fabric is hydrolyzed. The microvelvet is produced on the fabric surface. The softness, wrinkle resistance, moisture absorption, moisture permeability, air permeability increase and the breaking strength decrease. The bending rigidity of the ICLPAs-treated fabric is 0.003 1 cN·cm and drape coefficient is 1.97%. The crease recovery angle is 225°. The capillary effect (30 min) of the treated fabric is 11.3 cm. The air permeability is 372.1 L/(m 2·s) and the moisture penetrability is 2 303 g/(m 2·d).

Key words: silk, sanding, immobilization, protease, stability

CLC Number: 

  • TQ314.1

Tab.1

Factors and levels of orthogonal experiments for preparing ICLPAs"

水平 A B C D
沉降剂
体积/mL		 载体质量/
mg		 交联剂
质量分数/%		 交联时间/
min
1 2 10 1 20
2 4 20 2 40
3 6 30 3 60
4 8 40 4 80

Tab.2

Results of orthogonal experiments for preparing ICLPAs"

序号 A B C D 酶活回收率/%
1 1 1 1 1 52.4
2 1 2 2 2 57.2
3 1 3 3 3 63.2
4 1 4 4 4 54.4
5 2 1 2 3 64.8
6 2 2 1 4 71.2
7 2 3 4 1 59.6
8 2 4 3 2 61.2
9 3 1 3 4 84.4
10 3 2 4 3 87.6
11 3 3 1 2 92.8
12 3 4 2 1 68.4
13 4 1 4 2 81.3
14 4 2 3 1 61.1
15 4 3 2 4 86.8
16 4 4 1 3 78.4
K1 56.8 70.7 73.7 60.4
K2 64.2 69.3 69.3 73.1
K3 83.3 75.6 67.5 73.5
K4 76.9 65.6 70.7 74.2
R 26.5 10.0 6.2 13.8

Fig.1

SEM images of free protease (a) and ICLPAs(b)(×1 000)"

Fig.2

Formation scheme of carrier"

Fig.3

Reaction scheme of immobolized crosslinked protease aggregates"

Fig.4

Thermal stability of immobilized enzyme"

Fig.4

pH stability of immobilized enzyme"

Fig.6

Reusability of ICLPAs"

Fig.7

Images of untreated (a)and ICLPAs-treated samples(b)"

Tab.3

Bending rigidity, drape coefficient and crease recovery angle of fabrics"

样品 弯曲刚度/
(cN·cm)		 悬垂系数/
%		 折皱回复角(T+W)/
(°)
未处理 0.024 5 3.27 184
ICLPAs处理 0.003 1 1.97 225

Tab.4

Hygroscopicity, air permeability, moisture penetrability, thermal and wet resistance of fabrics"

样品 毛细效应(30 min)/cm 透湿性/(g·m-2·d-1) 湿阻/(m2·Pa·W-1) 热阻/(m2·℃·W-1) 透气性/(L·m-2·s-1)
未处理 9.1 1 711 5.112 0.018 6 339.5
ICLPAs处理 11.3 2 303 4.526 0.019 3 372.1
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