Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (11): 102-108.doi: 10.13475/j.fzxb.20191105407

• Dyeing and Finishing & Chemicals • Previous Articles     Next Articles

Structure and antibacterial properties of silica coated silver-copper nanoparticles

JIANG Xingmao, LIU Qi, GUO Lin()   

  1. School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, Hubei 430000, China
  • Received:2019-11-25 Revised:2020-08-13 Online:2020-11-15 Published:2020-11-26
  • Contact: GUO Lin E-mail:guolin2018@wit.edu.cn

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Abstract:

In order to study the synergistic antibacterial effect between bimetallic nanoparticles and prevent metal nanoparticle agglomeration, a ″dragon fruit″ type of high-load (50%) silica coated silver-copper bimetallic nanoparticle antibacterial agent (Ag-Cu/SiO2) was prepared using the aerosol one-step method. The structure of Ag-Cu/SiO2 was characterized by X-ray diffraction, transmission electron microscopy, electron spectroscopy, and the minimum inhibitory concentration (MIC) and time-kill curves of 50%Ag-Cu/SiO2 against Staphylococcus aureus and Escherichia coli was also studied. The formation of reactive oxygen species (ROS) in bacterial cells were subsequently investigated. The results show that the silver-copper bimetallic nanoparticles uniformly are dispersed in the spherical silica, presenting a "dragon fruit" structure. The Ag-Cu/SiO2 has better antibacterial properties than Cu/SiO2 and Ag/SiO2 with the same loading (50%). The MIC of Ag-Cu/SiO2 against both bacteria was 2 μg/mL and the growth of bacteria was fully inhibited within 24 h. The level of ROS produced by Ag-Cu/SiO2 is significantly higher than that of single metal nanoparticles and it causes the bacteria to die, indicating that the bimetallic nanoparticles have synergistic antibacterial effect.

Key words: silica, aerosol method, antibacterial agent, metallic nanoparticle, reactive oxygen species, antibacterial property

CLC Number: 

  • O614.12

Fig.1

Schematic diagram of process flow for preparing nanoparticles by aerosol method"

Fig.2

XRD pattern of Ag-Cu/SiO2 , Ag/SiO2 and Cu/SiO2"

Fig.3

EDS energy spectrum of silica-coated metal nanoparticles"

Fig.4

TEM images of silica-coated metal nanoparticles"

Fig.5

Particle size distribution of silica-coated metal nanoparticles"

Tab.1

Minimum inhibitory concentration of S. aureus and E.coli with different silica coated metal nanoparticles"

样品 S.aureus E.coli
SiO2 >256 >256
Cu/SiO2 >256 >256
Ag/SiO2 8 4
Ag-Cu/SiO2 2 2

Fig.6

Time-kill curves of S.aureus and E.coli treated with Ag-Cu/SiO2, Ag/SiO2 and Cu/SiO2"

Tab.2

Relative fluorescence intensity of S. aureus and E.coli with different silica coated metal nanoparticles"

样品 相对荧光强度
S.aureus E.coli
SiO2 1 222 1 915
Cu/SiO2 1 348 2 730
Ag/SiO2 2 177 4 404
Ag-Cu/SiO2 3 902 4 831
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