Journal of Textile Research ›› 2020, Vol. 41 ›› Issue (09): 162-166.doi: 10.13475/j.fzxb.20200202205

• Column: Biomedical Textile Materials and It′s Products • Previous Articles     Next Articles

Preparation and in vitro inflammation evaluation of polydopamine coated polypropylene hernia mesh

QIAO Yansha1,2, WANG Qian1,2, LI Yan1,2,3, SANG Jiawen1, WANG Lu1,2,3()   

  1. 1. College of Textiles, Donghua University, Shanghai 201620, China
    2. Key Laboratory of Textile Science & Technology of Ministry of Education, Donghua University, Shanghai 201620, China
    3. Key Laboratory of Textile Industry for Biomedical Textile Materials and Technology, Donghua University, Shanghai 201620, China
  • Received:2020-02-12 Revised:2020-05-29 Online:2020-09-15 Published:2020-09-25
  • Contact: WANG Lu E-mail:wanglu@dhu.edu.cn

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Abstract:

To reduce the possibility of inflammation with the use of polypropylene (PP) hernia mesh, dopamine was oxidized and self-polymerized for the preparation of an anti-inflammatory polydopamine (PDA) coating on the surface of PP monofilament. The micro-morphology, surface composition, water contact angle, coating stability, mechanical property, in vitro anti-oxidant and anti-inflammatory properties of PDA-PP were characterized and analyzed. The results show that PDA formed a coating of micro-nano particles with uniform distribution on PP fiber surfaces, and the coating changed physicochemical properties of PP mesh. Meanwhile, the original mechanical property of PP mesh is not affected by the PDA coating, and the antioxidant ability and good stability of the PDA coated PP mesh meet the needs of the initial inflammatory regulation. The protein expression level of the inflammation marker TNF-α and IL-6 at 24 h indicate a 93% and 100% lower expression levels for macrophages adhered PDA-PP mesh than that of the PP mesh, respectively. The PDA-PP mesh is expected for clinical evaluation studies next.

Key words: medical textiles, polypropylene mesh, inflammation, polydopamine, anti-oxidant, anti-inflammation

CLC Number: 

  • TS181.8

Fig.1

Apparent morphology of PP mesh (×10)"

Fig.2

SEM images of PP (a) and PDA-PP(b) (×3 000)"

Fig.3

FT-IR spectra of PP and PDA-PP"

Fig.4

XPS spectra of PDA-PP"

Fig.5

SEM images of PDA-PP at 1 d (a) and 3 d(b)"

Tab.1

Mechanical properties of PP and PDA-PP"

样品 断裂强度/(N·cm-1) 伸长率/%
横向 纵向 横向 纵向
PP 62.2±1.3 40.8±0.6 28.2±0.5 41.5±1.7
PDA-PP 63.6±2.1 41.6±0.8 27.2±1.1 42.0±0.6

Tab.2

CCK-8 absorbance value of L929 at 24 h"

样品 CCK-8吸光度值
PP 0.61±0.05
PDA-PP 0.55±0.04
空白对照样本 0.57±0.06

Tab.3

Concentration of TNF-α and IL-6 released from RAW 264.7"

样品 质量浓度/(pg·mL-1)
TNF-α IL-6
PP 189.13±2.13 4.8±0.6
PDA-PP 12.781±4.15 0
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