Journal of Textile Research ›› 2021, Vol. 42 ›› Issue (01): 40-45.doi: 10.13475/j.fzxb.20200301106

• Fiber Materials • Previous Articles     Next Articles

Preparation and characterization of polylactic acid-caprolactone/fibrinogen nanofiber based hernia mesh

YANG Gang1,2,3, LI Haidi1,2, QIAO Yansha1,2, LI Yan1,2(), WANG Lu1,2, HE Hongbing1,3   

  1. 1. College of Textiles, Donghua University, Shanghai 201620, China
    2. Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China
    3. Shanghai PINE & POWER Biotech Co., Ltd., Shanghai 201100, China
  • Received:2020-03-05 Revised:2020-07-30 Online:2021-01-15 Published:2021-01-21
  • Contact: LI Yan E-mail:yanli@dhu.edu.cn

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Abstract:

This paper reports on a research on preparation and characterization of a new tissue mesh engineered for hernia repair, where poly(L-lactide-co-caprolactone)/P(LLA-CL) and fibrinogen were blended in different proportions for electro-spinning. A series of P(LLA-CL)/fibrinogen composite nanofiber membranes were prepared, and their micromorphology, surface composition, wettability, mechanical properties and cellular compatibility were characterized and analyzed by scanning electron microscope, Fourier-transformed infrared spectroscopy, X-ray photoelectron spectroscopy, and cell experiments. The results showed that, after combining P(LLA-CL) with fibrinogen, the obtained fibrous membranes were all composed of nanoscale fibers and the porosity reached more than 60%. The addition of fibrinogen greatly improved the hydrophilicity of P(LLA-CL) and changed its original mechanical property. The results show that the P(LLA-CL)/fibrinogen nanofibrous membrane has a good cellular compatibility, and the high level porosity promotes the cell proliferation. In general, the nanofiber based hernia mesh demonstrates a great potential for tissue regeneration, providing a new hope for hernia repair.

Key words: hernia mesh, poly(L-lactide-co-caprolactone), fibrinogen, electrospinning, medical textiles

CLC Number: 

  • TS181.8

Tab.1

Cytotoxic response classification"

细胞毒性分级 RGR/% 细胞毒性
0 ≥100 无
I 75~99 无
II 50~74 轻度
III 25~49 中度
IV 1~24 中度
V <1 明显

Fig.1

SEM images of average diameter of P (LLA-CL)/fibrinogen electrospun membranes at different volume ratios (×3 000)"

Fig.2

Diameter distribution of fibers"

Tab.2

Porosity of P (LLA-CL)/fibrinogen composite electrospun membrane at different volume ratios"

P(LLA-CL)与纤维蛋白原体积比 孔隙率/%
1:0 56.56±1.32
2:1 63.25±2.07
1:1 69.31±1.77
1:2 71.22±1.94
0:1 72.87±2.31

Fig.3

FT-IR spectra of different samples"

Tab.3

Content of each element in P (LLA-CL)/fibrinogen electrospun membrane at different volume ratios"

P(LLA-CL)与纤维
蛋白原体积比		 含量 /%
C N O S
1:0 67.71 0.00 32.74 0.00
2:1 69.04 3.35 29.08 0.17
1:1 67.53 4.21 28.55 0.23
1:2 66.38 8.63 25.69 0.29
0:1 65.46 15.12 18.30 0.58

Fig.4

Water contact angles images of samples"

Tab.4

Water contact angels of P (LLA-CL)与fibrinogen electrospun membrane at different volume ratios"

P(LLA-CL)与纤维
蛋白原体积比		 接触角 /(°)
1:0 137.4±4.5
2:1 83.7±2.1
1:1 59.3±1.0
1:2 34.2±3.1
0:1 19.3±3.3

Fig.5

Stress-strain curves of electrospun membranes at different ratios"

Tab.5

Mechanical properties of P (LLA-CL)/fibrinogen composite electrospun membrane at different volume ratios"

P(LLA-CL)与纤维
蛋白原体积比		 断裂强度/
MPa		 弹性模量/
MPa		 断裂
伸长率/%
1:0 8.10±0.14 5.48±0.35 190.23±15.14
2:1 7.61±0.83 8.36±0.76 60.26±3.01
1:1 6.80±0.35 9.59±1.17 54.40±2.81
1:2 2.26±0.18 10.31±0.52 40.93±2.02
0:1 1.36±0.09 15.05±2.14 21.34±4.23

Fig.6

Cytotoxicity in different samples"

Tab.6

RGR values of different samples"

样品组别 相对增殖率/%
1 d 3 d 5 d
P(LLA-CL)/纤维蛋白原 81.3 87.8 93.8
阴性对照组 82.5 88.8 98.4
阳性对照组 21.6 9.3 7.9

Fig.7

OD values of L929 adhered on different substrates"

Tab.7

L929 adhesion rates on different substrates"

P(LLA-CL)与纤维
蛋白原体积比		 细胞黏附率 /%
4 h 24 h 72 h 120 h 168 h
1:0 15.9 19.1 25.3 31.1 34.3
2:1 9.0 18.8 24.2 62.5 78.7
1:1 10.8 20.6 29.6 50.5 69.0
1:2 9.0 17.0 27.1 46.9 59.6
0:1 9.0 18.1 25.6 41.5 62.8
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