Journal of Textile Research ›› 2021, Vol. 42 ›› Issue (04): 33-41.doi: 10.13475/j.fzxb.20200601109

• Fiber Materials • Previous Articles     Next Articles

Preparation and properties of electrospun collagen/polyethylene oxide nanofiber membranes

ZHAO Xinzhe, WANG Shaoxia, GAO Jing(), WANG Lu   

  1. College of Textiles, Donghua University, Shanghai 201620, China
  • Received:2020-06-01 Revised:2020-11-18 Online:2021-04-15 Published:2021-04-20
  • Contact: GAO Jing E-mail:gao2001jing@dhu.edu.cn

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Abstract:

In order to maintain and improve the structural stability of the collagen/polyethylene oxide nanofiber membranes under liquous conditions, the improved collagen/polyethylene oxide nanofiber membranes were prepared through electrospinning and by modifying the polymer mix with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide. The degree of swelling ratio, mechanical performance in dry and wet states, hemolytic performance and blood clotting property of the nanofiber membranes were measured and analyzed. The results show that the nanofibers is able to maintain its morphology after being soaked in phosphate buffered saline for 7 d with the swelling of fiber reduced (lower than 180%) and mechanical performance improved. In addition, the hemolysis percentage of crosslinked nanofiber membranes were considerably lower than 2%, indicating no damage to the red blood cells. More importantly, the blood clotting index of all crosslinked nanofiber membranes were all lower than 20% when compared to 48% with a non-crosslinked sample, indicating superior clotting property.

Key words: collagen nanofiber membrane, polyethylene oxide, crosslinking modification, carbodiimide, hemolysis, blood clotting property, electrospinning

CLC Number: 

  • TS171

Fig.1

Crosslinking degree of Col/PEO nanofiber membranes crosslinked by EDC/NHS and GA"

Fig.2

Crosslinking mechanism of GA (a) and EDC/NHS (b) crosslinked collagen"

Fig.3

Morphology of Col/PEO nanofiber membranes before and after immersed in PBS"

Fig.4

FT-IR spectra of Col/PEO nanofiber membranes before and after crosslinking"

Fig.5

DSC thermogram of collagen and Col/PEO nanofiber membranes before and after crosslinking"

Fig.6

Stress-strain curves of Col/PEO nanofiber membranes in dry (a) and hydrated (b) state"

Tab.1

Mechanical properties of Col/PEO membranes after crosslinking"

样品编号 断裂强度/MPa 断裂伸长率/%
干态 湿态 干态 湿态
1# 3.69±0.71 1.93±0.65 31.44±3.59 64.93±6.25
2# 4.92±0.49 3.06±0.71 18.57±2.72 40.30±5.31
3# 5.39±0.65 3.18±0.38 24.36±3.03 38.80±4.78
4# 5.42±0.73 3.85±0.94 14.76±2.61 56.63±5.45

Fig.7

Hemolysis test supernatant appearance (a) and hemolysis rate test results (b) of Col/PEO nanofiber membranes"

Fig.8

Blood clotting index of Col/PEO nanofiber membranes before and after crosslinking and medical gauze"

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