Journal of Textile Research ›› 2021, Vol. 42 ›› Issue (04): 114-120.doi: 10.13475/j.fzxb.20200703707

• Dyeing and Finishing & Chemicals • Previous Articles     Next Articles

Determination of luteolin content in carex meyeriana extract and its antibacterial properties

WANG Chunhong1,2(), YANG Lu1, HU Min1, WANG Xiaoyun1, WANG Lijian1   

  1. 1. School of Textile Science and Engineering, Tiangong University, Tianjin 300387, China
    2. Key Laboratory of Advanced Textile Composite Materials, Tiangong University, Tianjin 300387, China
  • Received:2020-07-15 Revised:2020-12-31 Online:2021-04-15 Published:2021-04-20

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Abstract:

In order to develop the application of carex meyeriana, and explore its antibacterial performance, the carex meyeriana extract was extracted by alcohol water mixture, and the basic performance of the extract with different alcohol water ratios were tested. The content of luteolin, an antibacterial substance, was tested by ultraviolet (UV) spectrophotometer and high performance liquid chromatography (HPLC) method, and the results of the two tests are compared. Its antibacterial rate was tested by in vitro antibacterial method. The results show that with the increase of the proportion of water in the extraction solvent, the conductivity of the extraction solution gradually increases and the pH value gradually decreases exhibiting acidity. The change trends of luteolin content detected by using UV spectrophotometer and HPLC method are the same. The content of luteolin decreases with the increase of the proportion of water in the extraction solvent, and the antibacterial rates of Escherichia coli and Staphylococcus aureus in the four samples with different alcohol water ratios were all higher than 70%, which indicates that the extract has good antibacterial properties.

Key words: carex meyeriana extract, ultraviolet spectrophotometry method, high performance liquid chromatography method, luteolin, in vitro antibacterial activity, antibacterial property

CLC Number: 

  • TS101.4

Tab.1

Medium configuration method"

培养基名称 牛肉膏
质量/g		 蛋白胨
质量/g		 溶剂
名称 体积/mL
A 0.6 1.0 提取液a 200
B 0.6 1.0 提取液b 200
C 0.6 1.0 提取液c 200
D 0.6 1.0 提取液d 200
E 0.9 1.5 蒸馏水 300

Tab.2

Test results of pH value and conductivity of different extracts"

提取液编号 pH值 电导率/(μS·cm-1)
a 6.39 192
b 6.26 322
c 5.94 471
d 5.69 546

Fig.1

Apparent color picture of extract. (a) Extract a; (b) Extract b; (c) Extract c; (d) Extract d"

Fig.2

Curve of UV spectrometric test for luteolin standard solvent"

Tab.3

Concentration of luteolin in different solvent extracts tested by UV spectrophotometer method"

提取液编号 吸光度 木犀草素质量浓度/(μg·mL-1)
a 1.375 29.22
b 0.123 1.36
c 0.121 1.31
d 0.110 1.07

Fig.3

HPLC chart for luteolin standard solvent"

Fig.4

Standard curve of HPLC for luteolin standard solvent"

Fig.5

HPLC chart of different extracts. (a) Extract a; (b) Extract b; (c) Extract c; (d) Extract d"

Tab.4

Content of luteolin in different extracts tested by HPLC"

提取液编号 峰面积/au·min 木犀草素的质量浓度/(μg·mL-1)
a 3 803 864 37.97
b 182 512 1.48
c 172 481 1.38
d 145 449 1.11

Fig.6

Antibacterial effect on Escherichia coli of carex meyeriana prepared by different ratios of alcohol to water. (a)Experimental group A; (b)Experimental group B; (c)Experimental group C; (d)Experimental group D; (e)Controlled group E"

Tab.5

Antibacterial rate of carex meyeriana extract to Escherichia coli"

平板序号 细菌计数平均值/(109 CFU·mL-1) 抑菌率/%
实验组A 4.24 90.6
实验组B 8.73 80.5
实验组C 9.40 79.2
实验组D 11.10 75.5
对照组E 45.30 —

Fig.7

Antibacterial effect on Staphylococcus aureus of carex meyeriana prepared by different ratios of alcohol to water. (a)Experimental group A; (b)Experimental group B; (c)Experimental group C; (d)Experimental group D; (e)Controlled group E"

Tab.6

Antibacterial rate of carex meyeriana extract to Staphylococcu saureus"

平板序号 细菌计数平均值/(109 CFU·mL-1) 抑菌率/%
实验组A 8.93 88.6
实验组B 14.70 81.2
实验组C 19.60 75.0
实验组D 19.90 74.6
对照组E 78.30 —
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