Journal of Textile Research ›› 2022, Vol. 43 ›› Issue (04): 15-19.doi: 10.13475/j.fzxb.20210302305

• Fiber Materials • Previous Articles     Next Articles

Hemostasis properties of silk fibroin materials under different types of hydrolysis

LEI Caihong1,2, YU Linshuang1, ZHU Hailin1,2, ZHENG Tao1, CHEN Jianyong1,2()   

  1. 1. College of Textile Science and Engineering(International Institute of Silk), Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China
    2. Zhejiang Provincial Key Laboratory of Fiber Materials and Manufacturing Technology, Hangzhou, Zhejiang 310018, China
  • Received:2021-03-05 Revised:2022-01-19 Online:2022-04-15 Published:2022-04-20
  • Contact: CHEN Jianyong E-mail:cjy@zstu.edu.cn

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Abstract:

Aiming at the improvement of hemostasis properties of silk fibroin materials fabricated by salt hydrolysis and enzyme hydrolysis method, the silk fibroin materials were prepared via a freeze-drying method. The molecular weight, water solubility and hemostatic effect of the resultant material were analyzed by gel filtration chromatography, water solubility test, coagulation factor test, platelet adhesion test, enzyme-linked immunosorbent assay and rat liver injury model. The results showed that compared with the salt hydrolysis method, the enzyme hydrolysis method could endow a lower molecular weight and better water soluble, which is conducive to activating the coagulation factor XII to promote endogenous coagulation. Moreover, the platelet adhesion and platelet factor 4 content increase significantly, the amount of bleeding from the rat liver is reduced with shortened bleeding time, indicating better hemostatic effect than that of Yunnan white medicine.

Key words: silk, silk fibroin, types of hydrolysis, salt hydrolysis, enzyme hydrolysis, hemostasis property

CLC Number: 

  • TQ341.5

Fig.1

APTT and PT values for two different hydrolysis systems of silk fibroin materials"

Fig.2

Absorbance value of silk fibroin materials produced by two different hydrolysis systems"

Fig.3

Hemostatic time and amount of bleeding for silk fibroin materials produced by two different hydrolysis systems in liver trauma model"

[1] YANG X, LIU W, LI N, et al. Design and development of polysaccharide hemostatic materials and their hemostatic mechanism[J]. Biomaterials Science, 2017, 5(12): 2357-2368.
doi: 10.1039/C7BM00554G
[2] 钱璐敏, 张斌. 可溶性止血医用棉纱布的制备及其性能[J]. 纺织学报, 2019, 40(5):102-106.
QIAN Lumin, ZHANG Bin. Preparation and characterization of soluble hemostatic medical cotton gauze[J]. Journal of Textile Research, 2019, 40(5):102-106.
[3] BANDYOPADHYAY A, CHOWDHURY S K, DEY S, et al. Silk: a promising biomaterial opening new vistas towards affordable healthcare solutions[J]. Journal of the Indian Institute of Science, 2019, 99(3): 445-487.
doi: 10.1007/s41745-019-00114-y
[4] 严佳, 李刚. 医用纺织品的研究进展[J]. 纺织学报, 2020, 41(9):191-200.
YAN Jia, LI Gang. Research progress on medical textiles[J]. Journal of Textile Research, 2020, 41(9):191-200.
[5] 钟红荣, 方艳, 包红, 等. 丝素基双层敷料的制备及其性能[J]. 纺织学报, 2020, 41(2):13-19.
ZHONG Hongrong, FANG Yan, BAO Hong, et al. Preparation and properties of silk fibroin based bilayer dressing materials[J]. Journal of Textile Research, 2020, 41(2):13-19.
[6] CHEN G, WE R, HUANG X, et al. Synthesis and assessment of sodium alginate-modified silk fibroin microspheres as potential hepatic arterial embolization agent[J]. International Journal of Biological Macromolecules, 2020, 155: 1450-1459.
doi: 10.1016/j.ijbiomac.2019.11.122
[7] HUANG X, FU Q, DENG Y, et al. Surface roughness of silk fibroin/alginate microspheres for rapid hemostasis in vitro and in vivo[J]. Carbohydrate Polymers, 2021, 253: 117256.
doi: 10.1016/j.carbpol.2020.117256
[8] ZHOU F, XUE Z, WANG J. Antihypertensive effects of silk fibroin hydrolysate by alcalase and purification of an ACE inhibitory dipeptide[J]. Journal of Agricultural and Food Chemistry, 2010, 58(11):6735-6740.
doi: 10.1021/jf101101r
[9] 郭少哲. 基于结构和分子量调控的丝素蛋白纯化方法的研究和应用[D]. 苏州: 苏州大学, 2016:11-12.
GUO Shaozhe. Insight into structure and molecular weight of silk fibroin for research and application[D]. Suzhou: Soochow University, 2016:11-12.
[10] LAN G, LU B, WANG T, et al. Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent[J]. Colloids & Surfaces B: Biointerfaces, 2015, 136:1026-1034.
[11] 程玮璐. 天然高分子基多功能止血复合敷料的制备及其性能研究[D]. 哈尔滨: 哈尔滨工业大学, 2016:35,60.
CHENG Weilu. Study on preparation and performance of natural polymer-based functional composite for hemostatic wound dressing[D]. Harbin: Harbin Institute of Technology, 2016:35,60.
[12] WU J, LEMARIÉ C A, BARRALET J, et al. Amphiphilic peptide-loaded nanofibrous calcium phosphate microspheres promote hemostasis in vivo[J]. Acta Biomaterialia, 2013, 9(11): 9194-9200.
doi: 10.1016/j.actbio.2013.06.023
[13] AVANTIKA G, HARIT P, MATTHEW R S, et al. Enzymes produced by autoactivation of blood factor XII in buffer: a contribution from the Hematology at Biomaterial Interfaces Research Group[J]. Biomaterials, 2015, 37: 1-12.
doi: 10.1016/j.biomaterials.2014.09.015
[14] TOULON P, ELOIT Y, SMAHI M, et al. In vitro sensitivity of different activated partial thromboplastin time reagents to mild clotting factor deficiencies[J]. International Journal of Laboratory Hematology, 2016, 38(4):389-396.
doi: 10.1111/ijlh.12499
[15] 王会敏, 潘吉勇, 杨冰, 等. 全溶性止血纤维的动物试验及临床应用研究[J]. 中国临床药理学与治疗学, 2006, 11(9): 1017-1020.
WANG Huimin, PAN Jiyong, YANG Bing, et al. Study of animal and clinical use on fully soluble hemo-static[J]. Chinese Clinical Pharmacology and Therapeutics, 2006, 11(9): 1017-1020.
[16] SMITH C W, ZAHER R, LOLA P, et al. TREM-like transcript 1: a more sensitive marker of platelet activation than P-selectin in humans and mice[J]. Blood Advances, 2018, 2(16): 2072-2078.
doi: 10.1182/bloodadvances.2018017756
[17] KUNDU B, SCHLIMP C J, NÜRNBERGER S, et al. Thromboelastometric and platelet responses to silk biomaterials[J]. Scientific Reports, 2014, 4(1): 2941-2953.
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