Journal of Textile Research ›› 2022, Vol. 43 ›› Issue (03): 71-77.doi: 10.13475/j.fzxb.20210305707

• Fiber Materials • Previous Articles     Next Articles

Preparation and performance of chitosan-based antibacterial gel

ZHANG Tao1, WANG Fuping1, CHEN Guobao1, WU Jiyu2, PANG Yani2, CHEN Zhongmin1()   

  1. 1. College of Pharmacy and Biological Engineering, Chongqing University of Technology, Chongqing 400054, China
    2. Chongqing Hualun Medical Equipment Co., Ltd., Chongqing 400039, China
  • Received:2021-03-15 Revised:2021-10-17 Online:2022-03-15 Published:2022-03-29
  • Contact: CHEN Zhongmin E-mail:chenzhongmin@cqut.edu.cn

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Abstract:

This research addresses the problems of low viscosity and poor antibacterial effect of the chitosan-sodium glycerophosphate system. The chitosan-sodium glycerophosphate system was used as the base material. After sodium carboxymethylcellulose was added to adjust the viscosity, sericin and silk fibroin were added separately to determine the optimal combination to prepare a composite gel. The antibacterial effect, the combination of each component, the antibacterial mechanism, cytotoxicity, and skin irritation of the composite gel were analyzed. The results show that the mixture of the 3% chitosan and 50% sodium glycerophosphate in the volume ratio of 9∶20 together with 1.5% sodium carboxymethyl cellulose demonstrates the moderate viscosity of (68 300±540) mPa·s, which was suitable for external use. When the mass fraction of sericin is 15%, the antibacterial effect of the composite gel is the best, and the antibacterial rate against Escherichia coli and Staphylococcus aureus is (96.20±3.40)% and (86.30±2.51)%, respectively. The gel has a stable structure, and it was found that the composite gel changes the permeability of the bacterial cell membrane, resulting in the inability of the bacteria to proliferate and antibacterial effects, without cytotoxicity and skin irritation.

Key words: chitosan, sericin, silk fibroin, composite gel, antimicrobial

CLC Number: 

  • TQ341.5

Fig.1

Antibacterial effects of different mass fractions of SS (a) and SF (b) composite gel extracts"

Fig.2

Antibacterial properties of different mass fractions of SS (a) and SF (b) composite gel extracts"

Fig.3

Infrared spectra of composite gel and its component"

Tab.1

Effect of different mass fraction extracts on integrity of bacterial cell membrane"

试样名称 浸提液质量
分数/%		 OD值 细胞膜透
过率/%
浸提液+细菌悬液+PI 25 0.222±0.025 23.67
50 0.305±0.018 31.47
75 0.431±0.031 45.94
100 0.546±0.036 58.17
氨苄青霉素+细菌悬液 +PI — 0.601±0.039 64.07
Triton X-100+细菌悬液+PI — 0.938±0.052 100
缓冲液+ 细菌悬液+PI — 0 0
浸提液+PI 100 0 0

Fig.4

Change of electrical conductivity of gel in 8 h after treatment of Escherichia coli"

Fig.5

Growth of L929 cells in extract medium containing different mass concentration"

Fig.6

Rat skin irritation response chart. (a) Skin reaction after applying formaldehyde; (b) Skin reaction after applying gel"

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