Abstract:

In order to explore the preparation conditions and biocompatibility of silk fibroin hydrogel materials, a controllable hydrogel system consisting of two myristic phosphatidylglycerol (DMPG) and degumming silk fibroin (SF) was prepared. The SF chain was oriented by electrostatic and hydrophobic interactions between phospholipids and SF chains β. The SF hydrogel scaffolds were obtained by folding structure transformation. The cell proliferation rate, cell differentiation rate, the growth rate of animal scaffold cells and animal scaffold degradation rate were compared by different molar concentration of scaffolds. The results showed that the higher the concentration of DMPG was, the shorter the preparation time of SF hydrogel scaffolds. The 15 mmol/L DMPG shortened the preparation time of SF hydrogel scaffolds from 7 d to 10 min. DMPG induced SF hydrogel demonstrated no cytotoxicity, and when DMPG was 10 mmol/L the cell proliferation rate was the highest. SF hydrogel scaffold with DMPG concentration of 10 mmol/L was injected into the subcutaneous tissue of the Hu sheep's ear. The SF hydrogel scaffold containing Hu sheep muscle satellite cells grew muscle in the animal body, and no muscle could be produced by simply injecting scaffolds or cells. SF hydrogel scaffolds possess excellent biocompatibility, and with the increase of SF hydrogel scaffolds in animal, the scaffold gradually degraded and disappeared, exposing the muscle tissue.

Key words: tissue engineering, silk fibroin, hydrogel, cell proliferation and differentiation, degumming method, silk, vascularization, biocompatibility