Journal of Textile Research ›› 2022, Vol. 43 ›› Issue (03): 24-30.doi: 10.13475/j.fzxb.20211102108

• Invited Column: Biomedical Textiles • Previous Articles     Next Articles

Preparation of exosome-functionalized shish-kebab fibrous membrane and its osteogenic differentiation ability

ZHANG Yu1,2, LIU Laijun1,2, LI Chaojing1,2, JIN Qiaoqiao3,4, XIE Qianyang3,5, LI Peilun3,6, WANG Fujun1,2(), WANG Lu1,2   

  1. 1. Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, China
    2. College of Textiles, Donghua University, Shanghai 201620, China
    3. College of Stomatology, Shanghai Jiaotong University, Shanghai 200011, China
    4. Department of Endodontics, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
    5. Department of Oral Surgery, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
    6. Department of Orthodontics, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, China
  • Received:2021-11-03 Revised:2022-01-03 Online:2022-03-15 Published:2022-03-29
  • Contact: WANG Fujun E-mail:wfj@dhu.edu.cn

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Abstract:

In order to improve the osteoconduction of synthetic resorbable membranes for guided bone regeneration, polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) shish-kebab nanofibrous membranes were prepared by electrospinning and solvent-induced crystallization. Exosomes were then immobilized on membranes via adhesion of polydopamine. The morphology, chemical composition, physicochemical and cellular osteogenic differentiation properties of the composite fibrous membranes were characterized. The shish-kebab structure was successfully induced on PCL/β-TCP nanofibers. The results show that membranes doubly modified by shish-kebab and polydopamine had the best surface wettability and excellent protein adsorption ability. Exosome-functionalized shish-kebab fibrous membrane increases the alkaline phosphatase activity of bone marrow mesenchymal stem cells due to the synergistic effect of shish-kebab structure, polydopamine, and exosome, which has good therapeutic potential for promoting bone healing in vivo.

Key words: nanofibrous membrane, guided bone regeneration, electrospinning, shish-kebab fibrous membrane, exosome, dopamine, polycaprolactone, bone reconstruction material

CLC Number: 

  • TS101.4

Fig.1

SEM images of PT5 and PT5SK fibrous membranes before and after PDA modification"

Fig.2

FT-IR spectra of β-TCP, PCL, PT5, PT5/PDA, PT5SK and PT5SK/PDA"

Fig.3

Water contact angles of PT5 and PT5SK fibrous membranes before and after PDA modification"

Fig.4

Protein adsorption of PT5, PT5SK, PT5/PDA and PT5SK/PDA fibrous membranes"

Fig.5

FE-TEM image (a) and particle size distribution (b) of BMSC-Exo"

Fig.6

ALP staining of rBMSCs osteogenic on fibrous membranes at 7 d and 14 d"

Fig.7

ALP activity of rBMSCs osteogenic on fibrous membranes at 7 d and 14 d"

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